TaqMan Advanced miRNA Assays

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that allows for exceptional detection of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.

  • Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA Assays.
  • Sensitive—unlike traditional pre-amplification, the miR-Amp primers in the ligation-based cDNA synthesis kit recognize the universal sequences added to every miRNA on the 5’ and 3’ ends, to help prevent amplification bias. Detect targets with as few as 60 copies of input microRNA (miRNA).
  • Specific—detect only mature miRNA and distinguish between closely related highly homologous miRNAs with our gold-standard TaqMan specificity.
  • Small sample input—detect and quantify mature miRNA from as little as 1 pg of total RNA or 2 μL of plasma or serum.
  • Versatile—compatible with tissue and biofluids including serum and plasma.


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Assay search tool—find and buy your TaqMan miRNA assays

taqman-array-cards-plate

Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format

Enable highly sensitive and specific quantification of mature miRNAs using qPCR in higher throughputs.

Learn more about formats ›


TaqMan miRNA Assay selection guide

Format

Recommended TaqMan assay/array

Single tube

TaqMan Advanced miRNA Assays
Requires only 1-10 nanograms of total RNA or equivalent and is ideal for a small number of assays (ideal for >10 targets with RT chemistry)
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96-well plate

TaqMan Advanced miRNA Assays
 Comprehensive collection of pre-plated, pre-designed panels to pinpoint your specific target; broad instrument compatibility
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384-well microfluidic card

TaqMan Advanced miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 2,300 miRNA targets from as little as 1 ng of total RNA
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Custom formatted TaqMan Advanced miRNA array cards

OpenArray plate

TaqMan Advanced miRNA OpenArray panels
Run three samples per QuantStudio 12K Flex OpenArray plate, translating up to 818 assays per sample (up to 758 unique assays)
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Custom plating Contact: customplating@thermofisher.com

Flexible content panels

TaqMan flexible content panels offer an easy way to select and design a combination of assays in the format that matches your experiment. We offer a wide range of flexible content panels.
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Custom assay services

Want us to produce a custom assay for you?
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Workflow

mirVana™ miRNA isolation kits

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

mirVana™ miRNA isolation kits

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

Species TaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set)

Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)  and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended fo use with TaqMan Advanced miRNA assays.

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

White paper: TaqMan Advanced miRNA Assays - identifying normalizers
Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays

Product Categories

Related Applications

Related Products

Data Analysis Tools

Species TaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set)

Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)  and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended fo use with TaqMan Advanced miRNA assays.

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

White paper: TaqMan Advanced miRNA Assays - identifying normalizers
Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays

Product Categories

Related Applications

Related Products

Data Analysis Tools

Resources

 White paper: TaqMan Advanced miRNA Assays
 Product Bulletin: TaqMan Advanced miRNA Assays
Applied Biosystems qPCR Analysis Modules
Software for microRNA and non-codingRNA analysis

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