TaqMan-Genotyping-assay

Tailor your SNP assay to your genotyping workflow with our custom design option

Applied Biosystems Custom TaqMan SNP Genotyping assays make it easy to utilize the power of TaqMan chemistry for detection of SNPs in any organism or genome where predesigned assays aren’t available. Integrate our gold-standard TaqMan chemistry, advanced assay design, and easy-to-use workflow into any of your genotyping applications such as screening, association, candidate region, candidate gene, or fine-mapping studies.

Our assay design pipeline is the key

The proprietary assay design process that powers our predesigned collection uses over 90+ parameters to generate optimal primer-probe designs that are wet-lab proven to maximize sensitivity and specificity. Leverage our secure assay design pipeline for your unique SNP targets by submitting confidential target sequences to the Custom TaqMan Assay Design Tool. This pipeline has generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data.

All custom assays use TaqMan minor groove-binding (MGB) probes for superior allelic discrimination, improved signal-to-noise ratios, and design flexibility. All custom human SNP genotyping assays are functionally tested using 20 unique DNA samples to ensure assay amplification and sample clustering.

Design and order your custom assays online

Our simple online tool walks you through the process. You begin by entering a sequence, then submit the chosen target sites for assay design. Upon notification of successful design, simply add the custom assay to your card.

Order assays 

Custom TaqMan SNP Genotyping assays

Cat. No. – Human Cat. No. –
Non-human
Size No. of 25 µL reactions (96 well) No. of 10 µL reactions (96 fast) No. of 5 µL reactions
(384 well)
4331349 4332077 Small; 40x 300 750 1,500
4332072 4332075 Medium; 40x 1,000 2,500 5,000
4332073 4332076 Large; 80x 2,400 6,000 12,000
Discrimination 5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies
Assay components Each assay contains:
  • 2 unlabelled PCR primers: forward and reverse (primers at 900 nM final concentration)
  • 1 Applied Biosystems VIC dye–MGB-labeled probe detects the Allele 1 sequence (probes at 200 nM final concentration)
  • 1 Applied Biosystems FAM dye–MGB labeled probe detects the Allele 2 sequence (probes at 200 nM final concentration)

Delivery times
Approximate delivery times are 4–6 days in the US and 6–10 days in Europe
Expiration date    5 years from date of manufacture
Shipping and storage conditions Assays ship at ambient temperature. Store at -15 to -25°C upon arrival.