TaqMan Fusion Assays

Accurate detection of fusion transcripts

This specialized set of TaqMan™ Gene Expression Assays is designed to detect fusion transcripts using real-time PCR. Certain fusion transcripts are considered rare and are not well characterized; therefore, detecting these rare events using a single technology can deliver ambiguous results. TaqMan Gene Expression Assays are a complementary solution to next-generation sequencing (NGS) panels, designed to serve as an orthogonal validation method for confirming NGS results.

  • Built from proven TaqMan Assay chemistry
  • Performance guaranteed for all pre-designed assays* 
  • Consists of a single tube, which contains a probe, forward primer, and reverse primer for each target for use in the simple, fast PCR workflow 
  • Use universal cycling conditions—run any combination of assays in parallel on a single real-time PCR instrument
Assay search tool - find & buy your single tube TaqMan Assays:
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Specifications
Pre-formulated assay (20x or 60x mix):
  • 2 unlabeled PCR primers (900 nM each final 1x reaction concentration; 150 nM final 1x reaction concentration for Primer Limited assays)
  • 1 FAM™ or VIC™ dye-labeled TaqMan™ MGB probe (250 nM final 1x reaction concentration)
Documentation: Available to download at thermofisher.com/taqmanfiles :
  • Protocol for TaqMan Gene Expression products
  • Product insert
  • Assay Information File
Assay Information File: Includes Sales Order Information, List of Contents (Assay ID, Part Number, Expiration Date, & Lot Number), Annotation Information (Gene Symbol, Gene Name, Entrez Gene ID, Transcript Accession & Location on Transcript) and Design Details (Context Sequence, Amplicon Size)
Tracking/identification:
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes
Shipping and storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

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