why choose TaqMan

Applied Biosystems TaqMan Marker and Reporter Assays enable researchers to reliably detect and quantitate DNA or RNA sequences encoding common marker and reporter proteins, such as green fluorescent protein (GFP) and its derivatives. They are designed using the same verified pipeline as TaqMan Gene Expression Assays. Together with a TaqMan master mix, these assays offer a fast, simple, and scalable technique for quantifying your markers and reporters.

TaqMan Marker and Reporter Assays can be used to:         

  • Check for successful cloning and/or transfection of your fluorescent fusion protein.
  • Determine the efficacy of a cellular delivery system by quantifying the amount of plasmids delivered to the cell
  • Quantify copy numbers of a transgene in cell lines
  • Detect the SeV genome and transgenes in cells reprogrammed using the CTS CytoTune 2.0/2.1 Sendai reprogramming vectors

Already know your target of interest? Search for a pre-designed TaqMan Assay for your reporter or marker using the tool below.

Assay search tool

As there are many variants of markers and reporters available, please confirm that the assay detects the target of interest by viewing the Interrogated Sequence in the Detail view of the assay. If uncertain, please contact Technical Support.

Looking to design your own custom TaqMan Assay for your marker or reporter? Use our custom assay design tool.


Specifications

Available assays
Expressed fluorescent protein reporters: CytoTune-IPS 2.0/2.1 Sendai Reprogramming Kit markers:
Enhanced GFP
YFP
GFP
mCherry
LacZ
Luciferase
AcGFP
AmCyan
DsRed-Monomer
E2-Crimson
HcRed1
ZsYellow
mBanana
mOrange2
mPlum
mRasberry
mStrawberry
tdTomato
SeV
KOS
Klf4
cMyc
L-Myc
Sox2
Oct3/4
Assay components Delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe and forward and reverse primers)
Assay suffix nomenclature _mr: assays that end in _mr indicate a TaqMan Marker and Reporter Assay
Available sizes

Our TaqMan Marker and Reporter Assays are available in four sizes:

  • Cat. No. 4448892: Extra Small (FAM labeled, 75 reactions, 20X, made to order)
  • Cat. No. 4351372: Small (FAM labeled, 360 reactions, 20X, made to order)
  • Cat. No. 4351370: Medium (FAM labeled, 750 reactions, 20X, made to order)
  • Cat. No. 4351368: Large (FAM labeled, 2900 reactions, 20X, made to order)
Shipping and storage Shipped at ambient temperature.
Store at –15 to –25ºC.
White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Related application

Gene Expression Analysis

Data analysis tools

Connect cloud-based platform—free to sign up
Desktop software—downloads can be found in the Support Center for your specific Applied Biosystems qPCR instrument
ExpressionSuite Software—free to download