Applied Biosystems Real-Time PCR Instruments
Celebrating over 20 years of innovation in real-time PCR
As a leader in PCR innovation, we offer you the gold standard in real-time PCR instrumentation. With our Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments. The Applied Biosystems QuantStudio family of instruments enables you to obtain the results you need, connect and collaborate with colleagues, and achieve your research goals.
Simple to use for all levels of experience | Easy controls for optimized experiments | High flexibility and intuitive design | High versatility and intuitive design | Maximum throughput, flexibility, and scalability | Absolutely attainable chip-based digital PCR | |
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Reactions per Run | 96 | 96 or 384 | 96 or 384 | 96 or 384 | 96, 384, or 12,228 | 1 per chip |
Block Format | 96-Well 0.2-mL or 96-Well Fast 0.1-mL | 96-Well 0.2-mL, 96-Well Fast 0.1-mL, or 384-Well | 96, 96 Fast, 384, upgradeable to TaqMan Array (interchangeable) | 96, 96 Fast, 384, TaqMan Array (inter- changeable) | 96, 96 Fast, 384, TaqMan Array, Applied Biosystems OpenArray (interchangeable) |
20,000 partitions/chip |
Color Combinations (Multiplexing) | Up to 4 | 96-well up to 21 384-well up to 5 |
Up to 5 | Up to 21 | Up to 21* | 2 (endpoint detection) |
Run Time | As fast as 30 min | As fast as 30 min | As fast as 30 min | As fast as 30 min | As fast as 30 min | ~30 seconds to read 1 chip |
Programmable Volumes | 1-200 μL | 1–200 μL | 1–200 μL | 1–200 μL | 1–200 μL | N/A |
Validated volumes | 10-100 µl (96-well 0.2mL) 10-30 µl (96-well 0.1mL) |
10-100 µl (96-well 0.2mL) 10-30 µl (96-well 0.1mL) 5-20 µl (384-well) |
5–100 μL | 1–100 μL | 33 nL–100 μL |
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Automation Compatible | No | No | Upgradable to automation capability | Yes | Yes | No |
Remote Monitoring | Yes | Yes | Yes | Yes | Yes | No |
On-Board Memory | Yes, >2,000 runs | Yes, >2,000 runs | Yes, >100 runs | Yes, >100 runs | Yes, >100 runs | Yes, >500 chips |
Email Notification | Yes | Yes | Yes | Yes | Yes | No |
Assist with 21 CFR Part 11 Compliance | Security | Security, auditing, e-signature package included | Security, auditing, e-signature package available separately | No | ||
Sensitivity | Down to 1 copy | |||||
Dynamic Range | Up to 10 logs** | 5 logs | ||||
Chemistry | All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye. | TaqMan and SYBR Green | ||||
Applications Available | Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, High Resolution Melt and Protein Thermal Shift, Pathogen Detection | Gene Expression, Genotyping, Copy Number Variation, Protein Detection, High Resolution Melt and Protein Thermal Shift, MicroRNA, Pathogen Detection | Gene Expression, miRNA, Genopyting, Copy Number Variation, Protein Thermal Shift, HRM, Pathogen Detection | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, MicroRNA, Pathogen Detection | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, MicroRNA, Pathogen Detection | Rare Mutation Detection, Quantification of molecular standards, Copy Number Variation, Absolute Quantification, Pathogen Detection, Digital PCR |
IVD Platform | No | No | No | Available in certain countries | No | No |
Temperature Uniformity | ± 0.4°C | ± 0.4°C | ±0.5°C | ±0.5°C | ±0.5°C | N/A |
Excitation Source | OptiFlex™ System with white LED light | OptiFlex System with white LED light | OptiFlex™ System with halogen lamp | OptiFlex System with halogen lamp | OptiFlex System with white LED light | OptiFlex System with white LED light |
Filters/Colors | 4 excitation filters (450–600 nm) 4 emission filters (500–640 nm) |
96-well: 6 excitation filters (450–670 nm) 6 emission filters (500–720 nm) 384-well: 5 excitation filters (450–650 nm) 5 emission filters (500–700 nm) |
5 excitation filters (450–650 nm) 5 emission filters (500–700 nm) |
6 excitation filters (450–670 nm) 6 emission filters (500–720 nm) |
6 excitation filters (450–670 nm) 6 emission filters (500–720 nm) |
3 excitation filters (450-600 nm) 3 emission filters (500-640 nm) |
Thermo Fisher Cloud | direct connection | direct connection | connect to cloud through computer | connect to cloud through computer | connect to cloud through computer | Direct connection |
Wi-Fi Connectivity | Yes | Yes | No | No | No | Yes |
Size | W 27 cm D 50 cm H 40 cm |
W 27 cm D 50 cm H 40 cm |
W 50.5 cm D 67.2 cm H 73.8 cm |
W 50.5 cm D 67.2 cm H 73.8 cm |
W 50.5 cm D 67.2 cm H 73.8 cm |
W 13.5 cm D 23.25 cm H 21 cm |
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*Duplex capabilities with OpenArray and multiplex capabilities for other formats. **Up to 7 logs with OpenArray. |
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Find the parts you need as well as part numbers for our legacy real-time PCR systems.
A wide range of software applications to help you get the most from your Applied Biosystems real-time PCR system.
Reaction tubes, 96-well and 384-well plates, and adhesive films for real-time PCR.
Applied Biosystems Spectral Calibration Kits and TaqMan® RNase P Instrument Verification Plates to help calibrate and verify the performance of your real-time PCR system.
Looking for a thermal cycler for end-point PCR?
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For Research Use Only. Not for use in diagnostic procedures.