Celebrating over 20 years of innovation in real-time PCR

As a leader in PCR innovation, we offer you the gold standard in real-time PCR instrumentation. With our Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments. The Applied Biosystems QuantStudio family of instruments enables you to obtain the results you need, connect and collaborate with colleagues, and achieve your research goals.

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Which real-time or digital PCR system is right for you?

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Block Format Type Colors Product Full Specs
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Compare QuantStudio real-time PCR & digital PCR systems
  Simple to use for all levels of experience Easy controls for optimized experiments High flexibility and intuitive design High versatility and intuitive design Maximum throughput, flexibility, and scalability Absolutely attainable chip-based digital PCR
 
 

 

 

 

 

 

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Reactions per Run 96 96 or 384 96 or 384 96 or 384 96, 384, or 12,228 1 per chip
Block Format 96-Well 0.2-mL or 96-Well Fast 0.1-mL 96-Well 0.2-mL, 96-Well Fast 0.1-mL, or 384-Well 96, 96 Fast, 384, upgradeable to TaqMan Array (interchangeable) 96, 96 Fast, 384, TaqMan Array (inter- changeable) 96, 96 Fast, 384, TaqMan Array, Applied Biosystems OpenArray
(interchangeable)		 20,000 partitions/chip
Color Combinations (Multiplexing) Up to 4 96-well up to 21
384-well up to 5		 Up to 5 Up to 21 Up to 21* 2 (endpoint detection)
Run Time As fast as 30 min As fast as 30 min As fast as 30 min As fast as 30 min As fast as 30 min ~30 seconds to read 1 chip
Programmable Volumes 1-200 μL 1–200 μL 1–200 μL 1–200 μL 1–200 μL N/A
Validated volumes 10-100 µl (96-well 0.2mL)

10-30 µl (96-well 0.1mL)		 10-100 µl (96-well 0.2mL)

10-30 µl (96-well 0.1mL)

5-20 µl (384-well)
 5–100 μL 1–100 μL 33 nL–100 μL 14.5 μL per chip
Automation Compatible No No Upgradable to automation capability Yes Yes No
Remote Monitoring Yes Yes Yes Yes Yes No
On-Board Memory Yes, >2,000 runs Yes, >2,000 runs Yes, >100 runs Yes, >100 runs Yes, >100 runs Yes, >500 chips
Email Notification Yes Yes Yes Yes Yes No
Assist with 21 CFR Part 11 Compliance Security Security, auditing, e-signature package included Security, auditing, e-signature package available separately No
Sensitivity Down to 1 copy
Dynamic Range Up to 10 logs** 5 logs
Chemistry All real-time PCR-based chemistries.  Flexibility for chemistries with or without passive reference dye. TaqMan and SYBR Green
Applications Available Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection Gene Expression, miRNA, Genopyting, Copy Number Variation, Protein Thermal Shift, HRM, Pathogen Detection Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, MicroRNA, Pathogen Detection Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, MicroRNA, Pathogen Detection, Digital PCR Rare Mutation Detection, Quantification of molecular standards, Copy Number Variation, Absolute Quantification, Pathogen Detection,  Digital PCR
IVD Platform No No No Available in certain countries No No
Temperature Uniformity ± 0.4°C ± 0.4°C ±0.5°C ±0.5°C ±0.5°C N/A
Excitation Source OptiFlex™ System with white LED light OptiFlex System with white LED light OptiFlex™ System with halogen lamp OptiFlex System with halogen lamp OptiFlex System with white LED light OptiFlex System with white LED light
Filters/Colors 4 excitation filters (450–600 nm)
4 emission filters (500–640 nm)		 96-well: 6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)

384-well: 5 excitation filters (450–650 nm)
5 emission filters (500–700 nm)		 5 excitation filters (450–650 nm)
5 emission filters (500–700 nm)		 6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)		 6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)		 3 excitation filters (450-600 nm) 3 emission filters (500-640 nm)
Thermo Fisher Cloud direct connection direct connection connect to cloud through computer connect to cloud through computer connect to cloud through computer Direct connection
Wi-Fi Connectivity Yes Yes No No No Yes
Size W 27 cm
D 50 cm
H 40 cm		 W 27 cm
D 50 cm
H 40 cm		 W 50.5 cm
D 67.2 cm
H 73.8 cm		 W 50.5 cm
D 67.2 cm
H 73.8 cm		 W 50.5 cm
D 67.2 cm
H 73.8 cm		 W 13.5 cm
D 23.25 cm
H 21 cm
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*Duplex capabilities with OpenArray and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray.

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Find the parts you need as well as part numbers for our legacy real-time PCR systems.

Reaction tubes, 96-well and 384-well plates, and adhesive films for real-time PCR.

Looking for a thermal cycler for end-point PCR?

A wide range of software applications to help you get the most from your Applied Biosystems real-time PCR system.

Applied Biosystems Spectral Calibration Kits and TaqMan® RNase P Instrument Verification Plates to help calibrate and verify the performance of your real-time PCR system.

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