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Celebrating over 20 years of innovation in real-time PCR

As a leader in PCR innovation, we offer you the gold standard in real-time PCR instrumentation. With our Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments. The Applied Biosystems QuantStudio family of instruments enables you to obtain the results you need, connect and collaborate with colleagues, and achieve your research goals.

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Compare real-time PCR systems

  Simple to use for all levels of experience Easy controls for optimized experiments
 
 

 

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Reactions per Run 96 96 or 384
Block Format 96-Well 0.2-mL or 96-Well Fast 0.1-mL 96-Well 0.2-mL, 96-Well Fast 0.1-mL, or 384-Well
Color Combinations (Multiplexing) Up to 4 96-well up to 21
384-well up to 5
Run Time As fast as 30 min As fast as 30 min
Programmable Volumes  
Validated volumes 10-100 µl (96-well 0.2mL)

10-30 µl (96-well 0.1mL)
10-100 µl (96-well 0.2mL)

10-30 µl (96-well 0.1mL)

5-20 µl (384-well)
Automation Compatible No No
Remote Monitoring
  •  
  •  
On-Board Memory  
Email Notification
  •  
  •  
Assist with 21 CFR Part 11 Compliance   Security, auditing, e-signature package included
Sensitivity Down to 1 copy
Dynamic Range Up to 10 logs**
Chemistry All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye
Applications Available:  
GeneExpression
 
 
Genotyping
 
 
Copy Number Variation
 
 
Protein Detection
 
 
MicroRNA
 
 
Pathogen Detection
 
 
HRM    
IVD Platform No Available in certain countries
Temperature Uniformity 0.4 "degree symbol"C
Excitation Source Bright white LED
Filters/Colors 4 excitation filters (450–600 nm)
4 emission filters (500–640 nm)
96-well: 6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)

384-well: 5 excitation filters (450–650 nm)
5 emission filters (500–700 nm)
Cloud-enabled system Yes
Wi-Fi Connectivity Yes
Size 27 x 50 x 40 cm
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  High flexibility and intuitive design Smart and connected High versatility and intuitive design Smarter productivity Maximum throughput, flexibility, and scalability
 
 

 

 

 

 

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Tabs 5 colors 5 colors 6 colors (21 filter combinations) 6 colors (21 filter combinations) 6 colors (21 filter combinations)
Reactions per Run 96 or 384 96 96 or 384 96 96, 384, or 12,228
Interchangeable Block Formats 96, 96 Fast, 384, upgradeable to TaqMan Array (interchangeable) 96-well (0.2 mL block) 96, 96 Fast, 384, TaqMan Array (inter- changeable) 96-well (0.2 mL block) 96, 96 Fast, 384, TaqMan Array, Applied Biosystems OpenArray
(interchangeable)
Throughput High High High High Very High
Dimensions (H x W x D) 73.8 x 50.1 x 66.1 cm 52.5 x 33.8 x 54.7 cm 73.8 x 50.1 x 66.1 cm 52.5 x 33.8 x 54.7 cm 73.8 x 50.1 x 66.1 cm
VeriFlex temperature control N/A Yes, 3 zones N/A Yes, 6 zones N/A
Excitation Source OptiFlex System with halogen lamp Bright white LED OptiFlex System with halogen lamp Bright white LED OptiFlex System with halogen lamp
Sensitivity Down to 1 copy
Dynamic Range Up to 10 logs**
21 CFR Part 11-enablement Optional security, auditing, e-signature package No Optional security, auditing, e-signature package Security, auditing, e-signature package † Optional security, auditing, e-signature package
Touch screen Yes Yes, interactive Yes Yes, interactive Yes
Cloud-enabled system No Yes No Yes No
Run Time As fast as 30 min
Chemistry All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications Available:  
GeneExpression
 
 
 
 
 
Genotyping
 
 
 
 
 
Copy Number Variation
 
 
 
 
 
Protein Detection
 
 
 
 
 
MicroRNA
 
 
 
 
 
Pathogen Detection
 
 
 
 
 
HRM
 
 
 
 
 
IVD Platform No No Available in certain countries No No
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*Duplex capabilities with OpenArray and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray.

† Module available in 2020

  Absolutely attainable chip-based digital PCR
 
 

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Reactions per Run 1 per chip
Block Format 20,000 partitions/chip
Color Combinations (Multiplexing) 2 (endpoint detection)
Run Time ~30 seconds to read 1 chip
Programmable Volumes N/A
Validated volumes 14.5 μL per chip
Automation Compatible No
Remote Monitoring No
On-Board Memory Yes, >500 chips
Email Notification No
Assist with 21 CFR Part 11 Compliance No
Sensitivity Down to 1 copy
Dynamic Range 5 logs
Chemistry TaqMan and SYBR Green
Applications Available Rare Mutation Detection, Quantification of molecular standards, Copy Number Variation, Absolute Quantification, Pathogen Detection,  Digital PCR
IVD Platform No
Temperature Uniformity N/A
Excitation Source OptiFlex System with white LED light
Filters/Colors 3 excitation filters (450-600 nm) 3 emission filters (500-640 nm)
Thermo Fisher Cloud Direct connection
Wi-Fi Connectivity Yes
Size W 13.5 cm
D 23.25 cm
H 21 cm
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Find the parts you need as well as part numbers for our legacy real-time PCR systems.

A wide range of software applications to help you get the most from your Applied Biosystems real-time PCR system.

Reaction tubes, 96-well and 384-well plates, and adhesive films for real-time PCR.

Applied Biosystems Spectral Calibration Kits and TaqMan® RNase P Instrument Verification Plates to help calibrate and verify the performance of your real-time PCR system.

Looking for a thermal cycler for end-point PCR?

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