Real-time PCR Reagents & Kits

Real-time PCR (also known as quantitative PCR or qPCR) is the simultaneous amplification and detection/quantification of nucleic acids using the polymerase chain reaction (PCR). qPCR is a versatile technique and a powerful tool to support your research applications. Find optimized real-time PCR master mixes, reagents, and kits to power your experiments here.

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Recommended qPCR master mixes by application

Chemistry

Application

Start with..

Applied Biosystems™ TaqMan™*

Gene
expression

TaqMan™ Fast Advanced Master Mix
for 2-step
TaqMan™ Fast Virus Master Mix  
for 1-step virus detection and gene expression

Applied Biosystems™ TaqPath™**
(Clinical-grade TaqMan)

Gene
expression

TaqPath™ qPCR Master Mix
for 2-step
TaqPath™ 1-step RT-qPCR Master Mix
for 1-step
TaqPath™ 1-step Multiplex Master Mix
for 1-step & multiplex

Genotyping

TaqPath™ ProAmp Master Mix
for human or animal samples only (for plant samples, please visit our Agrigenomics section)

Applied Biosystem™ SYBR™

Gene
expression

PowerUp SYBR™ Green Master Mix
Need more information?
  Good results for gene expression Optimized for broad qPCR applications Convenient and fast
     
  SYBR® Green Real-Time PCR Master Mixes

SYBR™ Green Real-Time PCR Master Mixes 		TaqMan® Real-Time PCR Master Mixes

TaqMan™ Real-Time PCR Master Mixes		 One-Step Real-Time Master Mixes

One-Step Real-Time PCR Master Mixes
Starting template DNADNARNA
ApplicationsGene expression
DNA quantitation (pathogen detection)
CHiP		Gene expression
DNA quantitation
CHiP (chromatin immunoprecipitation)
SNP genotyping
microRNA & small RNAs
Copy number variation
Pathway analysis
Mutation detection
Protein analysis
Multiplexing
Detection of limited samples (preamplification) 		Virus detection and quantification
High-throughput gene expression
High-throughput screening
RNA quantification
Primer design requiredyes  
RT required,
learn more ›		yesyes 
Resources  How do I choose between one-step and two-step?
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Specialized real-time PCR applications

Sample-to-answer
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required:

Looking for qPCR reagents for molecular diagnostic test development? 
See our molecular diagnostic section

Looking for qPCR multiplexing solutions? 
See our qPCR multiplex applications section

Real-time PCR master mix selection tool

Select from our full selection of optimized Applied Biosystems™ and Invitrogen™ real-time PCR reagents to find the one that’s right for your desired application, detection chemistry, format, and running mode.

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* For Research Use Only. 

** General purpose reagent. For laboratory use. (Can also be used for research.)