Master mixes for research, biopharmaceutical, and clinical applications—TaqMan, TaqPath, and SYBR Green
Applied Biosystems offers optimized real-time PCR master mixes, reagents, and kits for use cases from basic research to biopharmaceutical and clinical testing. To help ensure optimal results, our application-specific master mixes are specifically formulated for peak performance.
For gene expression analysis and virus detection, Applied Biosystems master mixes are available with both TaqMan and SYBR chemistries. For applications that require peak lot-to-lot performance consistency and product traceability, Applied Biosystems TaqPath master mixes are manufactured in an ISO 13485-certified and FDA-registered facility that adheres to the principles of current good manufacturing practices (cGMP). TaqPath master mixes are also available for genotyping applications, such as SNP and CNV analysis.
Want to evaluate the performance of our master mix yourself? Try before you buy!
Request a SYBR or TaqMan master mix sample for research applications
Request a TaqPath master mix sample for genotyping, clinical, or biopharma applications
Recommended qPCR master mixes by application
Chemistry | Application | Start with.. |
TaqMan* | Gene
| TaqMan Fast Advanced Master Mix |
| Viral detection | TaqMan Fast Virus 1-Step Master Mixes for viral RNA targets | |
TaqPath** | Gene | TaqPath qPCR Master Mix |
Genotyping | TaqPath ProAmp Master Mix | |
| Microbe & pathogen detection | TaqPath Bactopure Microbial Detection master mixes for microbial DNA targets TaqPath 1-step RT-qPCR Master Mix for viral RNA targets | |
SYBR Green | Gene |
| Chemistry | Dye | Probe | |
|---|---|---|---|
| Master mix options |  SYBR Green real-Time PCR master mixes |  TaqMan or TaqPath master mixes for DNA targets or 2-step RT-qPCR |  TaqMan or TaqPath master mixes for 1-step RT-qPCR |
| Input | DNA | DNA | RNA |
| Applications | Gene expression DNA quantitation (pathogen detection) CHiP | Gene expression DNA quantitation CHiP SNP genotyping miRNA & small RNAs Copy number variation Pathway analysis Mutation detection Protein analysis Multiplexing Detection of limited samples (preamplification) | Virus detection and quantification High-throughput gene expression High-throughput screening RNA quantification |
| Primer design required | yes | Not if using a predesigned TaqMan Assay | |
| RT required,
learn more › | yes | yes for 2-step RT-qPCR | |
| Resources | What’s the difference between TaqMan and SYBR Green chemistry? | How do I choose between one-step and two-step? | |
| Order now | Order now | Order now | |
Sample-to-answer
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required:
Looking for qPCR reagents for molecular diagnostic test development?
See our molecular diagnostic section
Looking for qPCR multiplexing solutions?
See our qPCR multiplex applications section
Select from our full selection of optimized Applied Biosystems™ and Invitrogen™ real-time PCR reagents to find the one that’s right for your desired application, detection chemistry, format, and running mode.
* For Research Use Only.
** General purpose reagent. For laboratory use. (Can also be used for research.)
* This promotion is open to customers in the United States (excluding Puerto Rico) and Canada. Offer only applies to orders placed on thermofisher.com. Discount available only on specified products. Discount will apply to orders received by Life Technologies no later than October 31, 2022, or until promotional supplies are depleted, whichever comes first. Discount applies to the list price in effect at the time the order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.
