Master Mixes for clinical and non-clinical applications—TaqMan, TaqPath and SYBR Green
Real-time PCR (also known as quantitative PCR or qPCR) is the simultaneous amplification and detection/quantification of nucleic acids using the polymerase chain reaction (PCR). qPCR is a versatile technique and a powerful tool to support your research applications. Find optimized real-time PCR master mixes, reagents, and kits to power your experiments here.
Want to evaluate our best performing master mixes?
Request a sample for non-clinical applications
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Recommended qPCR master mixes by application
Chemistry | Application | Start with.. |
Applied Biosystems TaqMan* | Gene | TaqMan Fast Advanced Master Mix |
Applied Biosystems TaqPath** | Gene | TaqPath qPCR Master Mix |
Genotyping | TaqPath ProAmp Master Mix | |
Applied Biosystem SYBR | Gene |
| Good results for gene expression | Optimized for broad qPCR applications | Convenient and fast | |
|---|---|---|---|
 SYBR™ Green Real-Time PCR Master Mixes |  TaqMan™ Real-Time PCR Master Mixes |  One-Step Real-Time PCR Master Mixes | |
| Starting template | DNA | DNA | RNA |
| Applications | Gene expression DNA quantitation (pathogen detection) CHiP | Gene expression DNA quantitation CHiP (chromatin immunoprecipitation) SNP genotyping microRNA & small RNAs Copy number variation Pathway analysis Mutation detection Protein analysis Multiplexing Detection of limited samples (preamplification) | Virus detection and quantification High-throughput gene expression High-throughput screening RNA quantification |
| Primer design required | yes | ||
| RT required,
learn more › | yes | yes | |
| Resources | How do I choose between one-step and two-step? | ||
| Order now | Order now | Order now |
Sample-to-answer
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required:
Looking for qPCR reagents for molecular diagnostic test development?
See our molecular diagnostic section
Looking for qPCR multiplexing solutions?
See our qPCR multiplex applications section
Select from our full selection of optimized Applied Biosystems™ and Invitrogen™ real-time PCR reagents to find the one that’s right for your desired application, detection chemistry, format, and running mode.
* For Research Use Only.
** General purpose reagent. For laboratory use. (Can also be used for research.)
