One-Step Real-Time RT-PCR Master Mixes

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One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.

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Which one-step real-time RT-PCR master mix is right for you?

Key applications	Gene expression analysis and influenza surveillance	Virus detection and high throughput gene expression analysis	Molecular diagnostics development	Multiplexing (gene expression, virus detection, and molecular Dx development)
Recommended master mix	SuperScript III Platinum One-Step qRT-PCR Kit	TaqMan Fast Virus 1-Step Master Mix	TaqPath 1-Step RT-qPCR Master Mix, CG	TaqPath 1-Step Multiplex Master Mix
Cycling mode/run time	Standard/~120 mins	Fast/~60 mins	Fast/~60 mins	Fast/~60 mins
Lot-to-lot consistency	Good	Better	Best	Best
Instrument compatibility	++	+++	+++	+++
Inhibitor tolerance	++	+++	+++	+++
Multiplexing	+	++	++	+++
Carryover contamination control	+	++	+++ (Contains UNG)	+++ (Contains UNG)
Reverse trascriptase	SuperScript III RT	Thermostable MMLV	Thermostable MMLV	Thermostable MMLV
Passive reference dye	ROX or No ROX	ROX	ROX	Mustang Purple or None
Labeled use	Research Use Only	Research Use Only	General Purpose Reagent	General Purpose Reagent
Format	3 tubes	1 tube	1 tube	1 tube
Concentration	2X	4X	4X	4X
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