One-Step Real-Time RT-PCR Master Mixes

One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.

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Which One-Step Real-Time RT-PCR Master Mix Is Right for You?

	Standard mode		Fast mode
	RNA-to-Ct™ 1-Step Kit		TaqMan® Fast Virus 1-Step Master Mix
Applications	RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Gene Expression Multiplexing		RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Gene Expression Multiplexing
Detection Chemistry	TaqMan® SYBR®		TaqMan®
Sensitivity	High		High
Cycling/Run Time	120 minutes		<60 min
Tolerant to PCR Inhibitors
Reverse Transcriptase	Array Script UP		Thermostable MMLV
ROX Reference Dye to Normalize Signal & Ensure Data Integrity
Price per 20 µL rxn	$1.13-1.58		$1.51-1.62
Format	2 Tubes TaqMan® RT-PCR Mix (2x) and TaqMan® RT Enzyme Mix (40x)		1 Tube (4X Mix)
	Order now		Order now