One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.

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Which one-step real-time RT-PCR master mix is right for you?

Key applicationsGene expression analysis and influenza surveillanceVirus detection and high throughput gene expression analysisMolecular diagnostics developmentMultiplexing (gene expression, virus detection, and molecular Dx development)
Recommended master mixSuperScript III Platinum One-Step qRT-PCR KitTaqMan Fast Virus 1-Step Master MixTaqPath 1-Step RT-qPCR Master Mix, CGTaqPath 1-Step Multiplex Master Mix
Cycling mode/run timeStandard/~120 minsFast/~60 minsFast/~60 minsFast/~60 mins
Lot-to-lot consistencyGoodBetterBestBest
Instrument compatibility+++++++++++
Inhibitor tolerance+++++++++++
Multiplexing ++++++++
Carryover contamination control++++++ (Contains UNG)+++ (Contains UNG)
Reverse trascriptaseSuperScript III RTThermostable MMLVThermostable MMLVThermostable MMLV
Passive reference dyeROX or No ROXROXROXMustang Purple or None
Labeled use Research Use OnlyResearch Use OnlyGeneral Purpose ReagentGeneral Purpose Reagent
Format3 tubes1 tube1 tube1 tube
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