SYBR-web-header

Applied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes greatly simplify your experiment setup as they contain everything you need: buffer, dNTPs, thermostable hot-start DNA polymerase, and, of course, SYBR Green dye. Just add your sample and PCR primer pair and you're ready to run your qPCR.

Which SYBR Green master mix is right for you?

SYBR Green master mix formulations have improved over the years, as researchers have demanded improved sensitivity, greater specificity, enhanced reproducibility, faster time-to-results, and protection from contamination. Although we offer many choices of SYBR Green master mixes, following are the recommended and highest-performing master mixes.

Recommended master mixPowerTrack SYBR Green Master MixPowerUp SYBR Green Master Mix
ApplicationsGene expression analysis
NGS library quantification
Microbe detection
ChIP DNA analysis
 
Gene expression analysis
NGS library quantification
Microbe detection
ChIP DNA analysis
 
Recommended primer Tm55–65°C60°C
Sample tracking dye (pipetting QC)YesNo
SpecificityHighHigh
SensitivityHighHigh
Recommended primer concentration300–800 nM300–800 nM
Preassembled reaction benchtop stabilityUp to 8 hoursUp to 72 hours
Hot startAntibody-mediatedDual-lock hot start mechanism
Cycling modeStandard (~60 min)
Fast (~30 min)
Standard (~60 min)
Fast (~30 min)
Carryover contamination controlIncludes heat-labile UNG and a blend of dTTP/dUTPIncludes heat-labile UNG and a blend of dTTP/dUTP
Instrument compatibilityCompatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), and Roche LightCycler™ 480 systemsCompatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene Mx3005P™ systems
 

Check price
or order now

Request a sample

Check price
or order now

Request a sample

 

Looking for more options?

See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below.

 single-copy sensitivitydual-lock technologycompatible with fast cyclingdUTP includedUNG includedROX72 hr stability pre-PCRComments
Applied Biosystems productsWide instrument compatibility, and the best choice for Applied Biosystems instruments. All mixes include ROX dye. 
PowerTrack SYBR Green Master Mix

 

••

 

Our newest and best mix. Designed to increase your efficiency and reduce pipetting errors via sample tracking dye. Formulated for excellent specificity, sensitivity, fast or standard cycling, and wide instrument compatibility.
PowerUp SYBR Green Master Mix

••

 

Formulated for tight, hot-start control of Taq polymerase, high specificity and sensitivity, fast or standard cycling, and wide instrument compatibility
Power SYBR Green Master Mix
   

 

  A solid SYBR Green master mix without UNG
SYBR Select Master Mix

 

••

  Our brightest mix. May be too bright for some older instruments.
Fast SYBR Green Master Mix  

  The original fast cycling SYBR Green master mix
SYBR Green Master Mix   

 

  The original SYBR Green master mix
MeltDoctor HRM Master Mix   

 

 

  Formulated specifically for high resolution melt (HRM) analysis
Thermo Scientific productsMixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, and high ROX available.
Luminaris Color HiGreen qPCR Master Mix

  

V

 

Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes
Maxima SYBR Green qPCR Master Mix   

 

V

  Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes
DyNAmo ColorFlash SYBR Green qPCR Kit

 

 

V

 

Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes
Absolute QPCR Mix, SYBR Green     

V

  Our only SYBR Green mix without dUTP/UNG.  Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes.
Invitrogen productsMixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX and high ROX available.
SYBR GreenER qPCR SuperMix   

V

  Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes
Express SYBR GreenER qPCR SuperMix  

••

V

  Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes
Platinum SYBR Green qPCR SuperMix-UDG   

V

  Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes

1. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.
2. Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube.