Reverse Transcription

We offer a selection of high-quality products for first-strand cDNA synthesis to meet your research needs. Choose from a variety of reverse transcription enzymes, formats, primers, and dNTP solutions that work best for your application. With our premium line of Invitrogen SuperScript Reverse Transcriptases, we have what you need for superior cDNA synthesis results with any type of template RNA.

Reverse transcription products

Stand-alone enzymes for maximum flexibility in reaction setup

 Superior enzyme for all RNAs, including difficult templatesValue enzyme for commonly used RNA templates
 Superscript IV Reverse TranscriptaseM-MLV-Reverse Transcriptase
Optimal reaction temp50–55°C37°C
Reaction time10 min50 min
cDNA yieldSuperiorAverage
SensitivitySuperiorAverage
Max cDNA lengthUp to 12 kbUp to 7 kb
Ability to work with degraded or inhibitor-containing RNAHighLow
Reduced RNase H activityYesNo
 Learn moreLearn more

Complete kits with all cDNA synthesis reaction components

 Superior choice for all RNAs, including difficult templatesValue choice for commonly used RNA templates
 SuperScript IV
First-Strand Synthesis System
High-Capacity cDNA Reverse Transcription Kit
Reverse transcriptaseSuperScript IVMultiScribe MuLV
Optimal reaction temp50–55°C37°C
Reaction time10 min50 min
cDNA yieldSuperiorAverage
SensitivitySuperiorAverage
Max cDNA lengthUp to 12 kbUp to 7 kb
Ability to work with degraded or inhibitor-containing RNAHighLow
Reduced RNase H activityYesNo
 Learn moreLearn more

Other cDNA synthesis kits for endpoint PCR applications

cDNA synthesis kits for the highest convenience in RT-qPCR setup

 Superior master mix for all RNA templatesSuperior master mix for all RNA templates, with fast and easy gDNA removalValue choice for commonly used RNA templates
 SuperScript IV VILO Master MixSuperScript IV VILO Master Mix with ezDNase EnzymeHigh-Capacity cDNA Reverse Transcription Kit
Reverse transcriptaseSuperScript IVSuperScript IVMultiScribe MuLV
Optimal reaction temp50°C50°C37°C
Reaction time10 min10 min120 min
Ability to work with degraded or inhibitor-containing RNAHighHighLow
Reduced RNase H activityYesYesNo
Primers includedRandom primers & oligo(dT)20Random primers & oligo(dT)20Random primers
Integrated gDNA removalNoYesNo
FormatAll RT reaction components premixedAll RT reaction components premixedRT reaction components in separate tubes
 Learn moreLearn moreLearn more

One-step RT-PCR kits for fast and simple workflow

 Superior choice for all RNAs, including difficult templatesValue choice for standard applications
 SuperScript IV One-Step RT-PCR SystemSuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
Reverse transcriptaseSuperScript IVSuperScript III
PCR enzymePlatinum SuperFi DNA DNA PolymerasePlatinum Taq DNA Polymerase
Optimal reaction temperature50-55°C55°C
Reaction time10 min30 min
SensitivitySuperiorAverage
Maximum target length13.8 kb4.5 kb
 Learn moreLearn more

Stand-alone enzymes for maximum flexibility in reaction setup

 Superior enzyme for all RNAs, including difficult templatesValue enzyme for commonly used RNA templates
 Superscript IV Reverse TranscriptaseM-MLV-Reverse Transcriptase
Optimal reaction temp50–55°C37°C
Reaction time10 min50 min
cDNA yieldSuperiorAverage
SensitivitySuperiorAverage
Max cDNA lengthUp to 12 kbUp to 7 kb
Ability to work with degraded or inhibitor-containing RNAHighLow
Reduced RNase H activityYesNo
 Learn moreLearn more

Complete kits with all cDNA synthesis reaction components

 Superior choice for all RNAs, including difficult templatesValue choice for commonly used RNA templates
 SuperScript IV
First-Strand Synthesis System
High-Capacity cDNA Reverse Transcription Kit
Reverse transcriptaseSuperScript IVMultiScribe MuLV
Optimal reaction temp50–55°C37°C
Reaction time10 min50 min
cDNA yieldSuperiorAverage
SensitivitySuperiorAverage
Max cDNA lengthUp to 12 kbUp to 7 kb
Ability to work with degraded or inhibitor-containing RNAHighLow
Reduced RNase H activityYesNo
 Learn moreLearn more

Other cDNA synthesis kits for endpoint PCR applications

cDNA synthesis kits for the highest convenience in RT-qPCR setup

 Superior master mix for all RNA templatesSuperior master mix for all RNA templates, with fast and easy gDNA removalValue choice for commonly used RNA templates
 SuperScript IV VILO Master MixSuperScript IV VILO Master Mix with ezDNase EnzymeHigh-Capacity cDNA Reverse Transcription Kit
Reverse transcriptaseSuperScript IVSuperScript IVMultiScribe MuLV
Optimal reaction temp50°C50°C37°C
Reaction time10 min10 min120 min
Ability to work with degraded or inhibitor-containing RNAHighHighLow
Reduced RNase H activityYesYesNo
Primers includedRandom primers & oligo(dT)20Random primers & oligo(dT)20Random primers
Integrated gDNA removalNoYesNo
FormatAll RT reaction components premixedAll RT reaction components premixedRT reaction components in separate tubes
 Learn moreLearn moreLearn more

One-step RT-PCR kits for fast and simple workflow

 Superior choice for all RNAs, including difficult templatesValue choice for standard applications
 SuperScript IV One-Step RT-PCR SystemSuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
Reverse transcriptaseSuperScript IVSuperScript III
PCR enzymePlatinum SuperFi DNA DNA PolymerasePlatinum Taq DNA Polymerase
Optimal reaction temperature50-55°C55°C
Reaction time10 min30 min
SensitivitySuperiorAverage
Maximum target length13.8 kb4.5 kb
 Learn moreLearn more
Need RNase Inhibitor to stabilize RNA molecules? Find out more
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Resources

Education

Review educational resources for reverse transcription basics, cDNA synthesis, enzyme selection, troubleshooting tips, and cDNA applications.

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View our webinar series to learn more about molecular biology topics such as reverse transcription, PCR, and cloning.

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OEM & customized cDNA solutions

Tailor-made performance tested products with a strict quality standards to meet your assay needs.

Support

PCR and cDNA Synthesis Support Center

Find tips, troubleshooting help, and resources for your endpoint PCR and cDNA applications.

Product Support Documentation

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Contact Us

Email or call our technical application scientists for additional questions regarding PCR enzymes and master mixes.