Phospho-Specific ELISA Kits

Phospho-specific ELISA kits (phosphoELISA™ Kits) are high-quality ELISA kits, designed for researchers studying intracellular proteins involved in signaling pathways. Phosphorylation ELISA kits are designed to deliver accurate, sensitive, and fast protein quantitation of total and phosphorylated, modified, or cleavage site-specific proteins in a broad range of sample types.

Popular phospho-specific ELISA kit products

STAT3 [pY705] Phospho-ELISA Kit, Multispecies
Tau [pS396] Phospho-ELISA Kit, Human
CREB [pS133] Phospho-ELISA Kit, Human

Advantages of phospho-ELISA (phosphoELISA™) kits include:

  • Specificity–two antibodies directed against a target provides better specificity than western blotting
  • Sensitivity–more sensitive than western blotting
  • Quantitation–generate quantitative data, in contrast to western blotting
  • Fast results–96-well format, results in about 4 hours, no densitometry analysis needed

Other popular phospho-specific ELISA kits

Visit “Find ELISA kits by target” to search and select from all available phospho-specific ELISA kits

Phospho ERK ELISA kit demo video

Phospho p38 ELISA kit demo video

Phosphorylation ELISA kits

Abnormal phosphorylation states are implicated in many human diseases, including cancer. Studying the phosphorylation and post-translational modification of key protein targets is essential to better understanding the mechanisms behind disease.

We have phosphorylation ELISA kits for many of the key targets and signaling pathways implicated in various diseases, including STAT3, CREB, PRAS40, p38, AMPK alpha, PTK2, Caspase 3 signaling. These kits are manufactured to help ensure excellent quality and reproducibility. The kits must meet quality-controlled specifications for sensitivity, dynamic range, precision, specificity, recovery, and lot-to-lot consistency.

General schematic of phosphoELISA™ protocol. Capture antibodies are precoated on the bottom of a 96-well plate. The cell extract or standard is added to the wells and incubated to allow target proteins to bind. The wells are then washed to remove unbound material, and the detection antibody is added and incubated to form a sandwich around the protein of interest. HRP anti-rabbit antibody is then added and incubated to allow binding to the rabbit-derived detection antibody. Next, the chromogen substrate for HRP is added, and the subsequent enzymatic reaction turns the solution blue. Finally, the reaction is stopped, turning the solution yellow in proportion to the amount of target protein in the sample. Absorbance is read in a microplate reader at 450 nm.


Figure 1. Confirmation of western blotting data with quantitative results by phosphoELISA™ assay. (A) Quantitative data obtained using the STAT5a [pY694] Human ELISA Kit (Cat. No. KHO0761).


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