We offer a variety of streptavidin- and NeutrAvidin-coated plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule.
The figure above demonstrates coupling of a biotinylated protein to the covalently bound streptadivin. After a prewash, simply add the biotinylated target molecule in an appropriate buffer. In a short incubation step, the biotinylated molecule will bind to the streptavidin molecule.
NeutrAvidin protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.
Biotinylation reagents are available for targeting specific functional groups or residues, including primary amines, sulfhydryls, carboxyls, and carbohydrates. Photoreactive biotin compounds that react nonspecifically upon exposure to ultraviolet (UV) light are also available and expand the scope of the molecules that may be biotinylated.
A selection of FAQs is listed below. For more technical information and FAQs, please visit our Solid Phase Guide ›
When working with the Immobilizer streptavidin plates, instant coupling of biotinylated biomolecules will occur. The Immobilizer streptavidin plate is especially designed and optimized for detection of biotinylated oligonucleotides, peptides, and proteins.
We recommend using transparent polystyrene plates and strips for colorimetric assays, white polystyrene plates for bio and chemiluminescence assays, and black polystyrene plates for fluorescence assays.
Coupling of the target molecule to the streptavidin proceeds very fast. Results show that incubation with gentle agitation for two hours at room temperature ensures that all available active sites are saturated.
For Research Use Only. Not for use in diagnostic procedures.