Plates for solid-phase nucleic acid assays
Simplify PCR assisted hybridization assays by performing the PCR amplification and detection steps in the same well. We offer a range of plates to optimize your Solid Phase PCR, DIAPOPS (Detection of Immobilized Amplified Products), PCR-ELISA, and nucleic acid hybridization assays.
Nucleic acid assay plate types
Nunc NucleoLink Breakable Modules are ideal for hybridization assays due to the heat resistant resin. The unique design offers error-free flexibility; each well locks in the frame and maintains the same height to provide accurate reading and efficient washing.
- Heat-stable wells (120°C) with excellent thermal transfer properties
- Simplifies PCR assisted hybridization assays; perform the PCR amplification and detection steps in the same well. No need for special real-time PCR equipment
- Use in place of time-consuming methods such as gel electrophoresis and Southern blotting
- Usable with any “ELISA-like” detection and measurement method
- Compatible with standard microplate readers
- Consists of 1 x 8 strips to be fitted into a 96-well format frame
- V-shaped wells with flat, readable bottoms and thin walls (0.35mm)
- Working volume: Strips 100μL
The CovaLink surface is designed to facilitate the coupling of molecules bearing a free carboxyl or phosphate group. Therefore, peptides, haptens, and DNA can be coupled. The surface uses a spacer arm to increase accessibly, thereby enhancing overall surface reactivity.
DNA can be bound covalently to CovaLink NH strips. The DNA molecules are bound exclusively at the 5’- end by a phosphoramidate bond. Immobilization of more than 1 pmol is possible. When DNA coated CovaLink NH strips are used as the solid phase for hybridization assays, detection of less than 15·10–18 moles is possible.
The Immobilizer™ Amino surface forms stable covalent bonds between its electrophilic groups and the biomolecule's free amino acids or sulfhydryl groups. Using its unique spacer arm chemistry, the surface provides extremely low non-specific binding to improve assay sensitivity. With no need for an ancillary coupling agent, this surface can simplify your assay development by eliminating the need for a time-consuming blocking step.
The Immobilizer Amino surface is ideal:
- If your biomolecule does not bind well to a passive surface and it possesses one or more free primary amino or sulfhydryl groups (peptides, oligonucleotides, proteins, proteoglycans)
- To obtain a highly sensitive assay with excellent reproducibility and low background
- To minimize the use of a coating reagent
- To reduce the number of steps required to prepare plates
- To avoid unwanted reactivity associated with a blocking reagent
- For nucleic acid hybridization assays
We offer a wide range of avidin-coated plates to capture biotinylated nuclec acids, for use in PCR-ELISA and nucleic acid hybridization assays.
For technical notes and protocols for using nucleic acid assay plates please view the Solid Phase Guide ›
Random primer labeling kits, biotin labeling and detection, enzymes, and labeled nucleotides.
For Research Use Only. Not for use in diagnostic procedures.