ChIP, RIP, and Protein-Nucleic Acid Pull-Down Products
Chromatin immunoprecipitation (ChIP) assays are performed to identify regions of the genome with which DNA-binding proteins, such as transcription factors and histones, associate. In ChIP assays, proteins bound to DNA are temporarily crosslinked and the DNA is sheared prior to cell lysis. The target proteins are immunoprecipitated along with the crosslinked nucleotide sequences, and the DNA is then removed and identified by PCR, sequenced, applied to microarrays ,or analyzed in some other way.
RNA immunoprecipitation uses an approach similar to ChIP, except that RNA-binding proteins are immunoprecipitated instead of DNA-binding proteins. Immunoprecipitated RNAs can then be identified by RT-PCR and cDNA sequencing.
MAGnify™ Chromatin Immunoprecipitation System provides a faster and more reproducible solution for Chromatin Immunoprecipitation (ChIP) and includes all reagents needed to perform ChIP with an antibody of interest.
ChIP assay procedure
Chromatin Immunoprecipitation assays (ChIP assays) identify links between the genome and the proteome by monitoring transcription regulation through histone modification (epigenetics) or transcription factor:DNA binding interactions. The strength of ChIP assays is their ability to capture a snapshot of specific protein:DNA interactions occurring in a system and to quantify the interactions using quantitative polymerase chain reaction (qPCR).
The chromatin immunoprecipitation (ChIP) assay is a powerful method for analyzing epigenetic modifications and genomic DNA sequences bound to specific regulatory proteins.
Chromatin IP experiments require a variety of proteomics and molecular biology methods including crosslinking, cell lysis (protein-DNA extraction), nucleic acid shearing, antibody-based immunoprecipitation, DNA sample clean-up and PCR. Additional techniques such as gel electrophoresis are usually used during optimization experiments to validate specific steps.
For Research Use Only. Not for use in diagnostic procedures.