Magnetic bead use for IP is skyrocketing

The publication trends for immunoprecipitation say it all. Recent years have seen a skyrocketing increase in the number of publications citing the use of magnetic beads (and Invitrogen™ Dynabeads™ products in particular) vs. other technologies and products (e.g., Sepharose™ resin from GE Healthcare) for immunoprecipitation procedures. Why? Dynabeads magnetic beads have the following advantages:

  • Higher reproducibility—handling consistency leads to more consistent results (see photo)
  • Easier handling—~30 min protocol and no centrifugation helps make magnetic beads popular for those doing IP on a regular basis
  • Almost no background—magnetic beads have minimal non-specific binding, and no pre-clearing is necessary
  • Antibody savings—all binding occurs on bead outer surface, helping you conserve precious antibodies
  • Lower signal-to-noise—easy and efficient washing helps ensure superior contaminant removal
  • Gives you more control—designed to help you balance capacity/yield, reproducibility, and purity and reduce overall cost
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Below is a selection of citations for the use of Dynabeads magnetic beads in various applications. You can sort the table using the column arrows or search the table using the search box.

Additional citations can be found in our Citations and References database. The database can be searched using a product catalog number or keyword. For example, searching with the term "10001D" will return a list of journal articles that cite the use of Dynabeads Protein A for Immunoprecipitation (Cat. No. 10001D) and searching with the term "dynabeads" will return a list of journal articles that cite the use of any Dynabeads product. Note that the Citations and References database on lifetechnologies.com contains only the small subset of scientific journal articles that cite the use of our products. Many more can be found via targeted searches of the PubMed™ database and similar.

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Title Year Journal Application
Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1 2014 Nat Commun Dynabeads Protein A
Activation of TAK1 by MYD88 L265P drives malignant B-cell growth in non-Hodgkin lymphoma 2014 Blood Cancer J Dynabeads Protein A
MafB promotes atherosclerosis by inhibiting foam-cell apoptosis 2014 Nat Commun Dynabeads Protein A
Cyclin D1-Cdk4 controls glucose metabolism independently of cell cycle progression 2014 Nature Dynabeads Protein A
MYPT1 regulates contractility and microtubule acetylation to modulate integrin adhesions and matrix assembly 2014 Nat Commun Dynabeads Protein A
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2 2014 Nature Dynabeads Protein A
The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation 2014 Nat Commun Dynabeads Protein A
Nuclear localization in the biology of the CD40 receptor in normal and neoplastic human B lymphocytes 2006 J Biol Chem Dynabeads Protein A
Androgen receptor remains critical for cell-cycle progression in androgen-independent CWR22 prostate cancer cells 2006 Am J Pathol Dynabeads Protein A
NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly 2006 J Cell Biol Dynabeads Protein A
Identification of a protein subset of the anthrax spore immunome in humans immunized with the anthrax vaccine adsorbed preparation 2005 Infect Immun Dynabeads Protein A
Constitutive NF-kappaB and NFAT activation in aggressive B-cell lymphomas synergistically activates the CD154 gene and maintains lymphoma cell survival 2005 Blood Dynabeads Protein A
Interleukin-21 receptor gene induction in human T cells is mediated by T-cell receptor-induced Sp1 activity 2005 Mol Cell Biol Dynabeads Protein A
The survival of motor neurons protein determines the capacity for snRNP assembly: biochemical deficiency in spinal muscular atrophy 2005 Mol Cell Biol Dynabeads Protein A
Gemins modulate the expression and activity of the SMN complex 2005 Hum Mol Genet Dynabeads Protein A
Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts 2005 J Cell Sci Dynabeads Protein A
ZW10 links mitotic checkpoint signaling to the structural kinetochore 2005 J Cell Biol Dynabeads Protein A
Reduction of total E2F/DP activity induces senescence-like cell cycle arrest in cancer cells lacking functional pRB and p53 2005 J Cell Biol Dynabeads Protein A
Repair of U/G and U/A in DNA by UNG2-associated repair complexes takes place predominantly by short-patch repair both in proliferating and growth-arrested cells 2004 Nucleic Acids Res Dynabeads Protein A  
A plant snoRNP complex containing snoRNAs, fibrillarin, and nucleolin-like proteins is competent for both rRNA gene binding and pre-rRNA processing in vitro 2004 Mol Cell Biol Dynabeads Protein A
The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes 2003 J Virol Dynabeads Protein A
Parallel activation of phosphatidylinositol 4-kinase and phospholipase C by the extracellular calcium-sensing receptor 2002 J Biol Chem Dynabeads Protein A
Interaction with 14-3-3 proteins promotes functional expression of the potassium channels TASK-1 and TASK-3 2002 J Physiol Dynabeads Protein A
Heterogeneity in the modification and involvement of chromatin components of the CpG island of the silenced human CDH1 gene in cancer cells 2002 Nucleic Acids Res Dynabeads Protein A
Inhibition of poly(ADP-ribosyl)ation induces DNA hypermethylation: a possible molecular mechanism 2002 FASEB J Dynabeads Protein A
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry 2014 Sci Rep Dynabeads Protein G
Differential affinity of FLIP and procaspase 8 for FADD's DED binding surfaces regulates DISC assembly 2014 Nat Commun Dynabeads Protein G
MYPT1 regulates contractility and microtubule acetylation to modulate integrin adhesions and matrix assembly 2014 Nat Commun Dynabeads Protein G
Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C 2014 Nat Commun Dynabeads Protein G
ARID1B is a specific vulnerability in ARID1A-mutant cancers 2014 Nat Med Dynabeads Protein G
Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus 2014 Cell Res Dynabeads Protein G
A recently evolved class of alternative 3'-terminal exons involved in cell cycle regulation by topoisomerase inhibitors 2014 Nat Commun Dynabeads Protein G
Regulation of Mdm2 protein stability and the p53 response by NEDD4-1 E3 ligase 2014 Oncogene Dynabeads Protein G
PVT1 dependence in cancer with MYC copy-number increase 2014 Nature Dynabeads Protein G
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2 2014 Nature Dynabeads Protein G
The Bacillus subtilis DivIVA protein has a sporulation-specific proximity to Spo0J 2006 J Bacteriol Dynabeads Protein G
Dpb11, the budding yeast homolog of TopBP1, functions with the checkpoint clamp in recombination repair 2006 Nucleic Acids Res Dynabeads Protein G
Mpp4 recruits Psd95 and Veli3 towards the photoreceptor synapse 2006 Human Mol Genet Dynabeads Protein G
Nitration and inactivation of IDO by peroxynitrite 2006 J Immunol Dynabeads Protein G
E-cadherin regulates the association between beta-catenin and actinin-4 2005 Cancer Res Dynabeads Protein G
Identification of a protein subset of the anthrax spore immunome in humans immunized with the anthrax vaccine adsorbed preparation 2005 Infect Immun Dynabeads Protein G
Overexpression of an anti-CD3 immunotoxin increases expression and secretion of molecular chaperone BiP/Kar2p by Pichia pastoris 2005 Appl Environ Microbiol Dynabeads Protein G
Novel cardioprotective role of a small heat-shock protein, Hsp20, against ischemia/reperfusion injury 2005 Circulation Dynabeads Protein G
The polyglutamine neurodegenerative protein ataxin 3 regulates aggresome formation 2005 Proc Natl Acad Sci U S A Dynabeads Protein G
Tissue transglutaminase is a multifunctional BH3-only protein 2004 J Biol Chem Dynabeads Protein G
A prototype antibody microarray platform to monitor changes in protein tyrosine phosphorylation 2004 Mol Cell Proteomics Dynabeads Protein G
Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro 2004 Mol Cell Biol Dynabeads Protein G
Multiprotein complex containing succinate dehydrogenase confers mitochondrial ATP-sensitive K+ channel activity 2004 Proc Natl Acad Sci U S A Dynabeads Protein G
Comprehensive search for HNF-1beta-regulated genes in mouse hepatoma cells perturbed by transcription regulatory factor-targeted RNAi 2004 Nucleic Acids Res Dynabeads Protein G
The polo box is required for multiple functions of Plx1 in mitosis 2004 J Biol Chem Dynabeads Protein G
The role and regulation of the preRC component Cdc6 in the initiation of premeiotic DNA replication 2004 Mol Biol Cell Dynabeads Protein G
Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo 2004 Mol Cell Biol Dynabeads Protein G
Protein sorting in the late Golgi of Saccharomyces cerevisiae does not require mannosylated sphingolipids 2004 J Biol Chem Dynabeads Protein G
Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes 2002 Mol Biol Cell Fishing for large protein complexes; downstream mass spectrometry
Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei 1998 Mol Cell Biol Fishing for large protein complexes
Binding of CaMKII to the giant muscle protein projectin: stimulation of CaMKII activity by projectin 2002 Biochim Biophys Acta IP and co-IP
HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction 2000 Genes Dev IP and co-IP
A rapid and sensitive immunoassay for tumor necrosis factor using magnetic monodisperse polymer particles 1990 J Immunol Methods IP and co-IP, bio- and imunoassays
The use of paramagnetic beads for the detection of major histocompatibility complex class I and class II antigens 1991 Biotechniques IP and co-IP
Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2 2001 Mol Biol Cell IP and co-IP
Sorting of MHC class II molecules and the associated invariant chain (Ii) in polarized MDCK cells 1997 J Cell Sci IP and co-IP
A casein kinase I isoform is required for proper cell cycle progression in the fertilized mouse oocyte 1997 J Cell Sci Isolation and immobilization of active enzymes
Parallel activation of phosphatidylinositol 4-kinase and phospholipase C by the extracellular calcium-sensing receptor 2002 J Biol Chem Isolation and immobilization of active enzymes
Prenucleolar bodies contain coilin and are assembled in Xenopus egg extract depleted of specific nucleolar proteins and U3 RNA 1997 J Cell Sci Depletion of proteins
Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts 1995 J Cell Sci Depletion of proteins
Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells 2002 J Biol Chem Protein isolation through tags; downstream mass spectrometry
Coupling of MALDI-TOF mass analysis to the separation of biotinylated peptides by magnetic streptavidin beads 1996 Anal Chem Downstream mass spectrometry
XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle 1997 J Cell Biol Downstream mass spectrometry
Sugar profiling proves that human serum erythropoietin differs from recombinant human erythropoietin 2001 Blood Doping analysis
Magnetic DNA affinity purification of yeast transcription factor tau--a new purification principle for the ultrarapid isolation of near homogeneous factor 1989 Nucleic Acids Res DNA and RNA binding proteins
Magnetic DNA affinity purification of yeast transcription factor 1993 Methods Enzymol DNA and RNA binding proteins
FMR1 protein: conserved RNP family domains and selective RNA binding 1993 Science DNA and RNA binding proteins
Solid phase DNase I footprinting: quick and versatile 1994 Nucleic Acids Res Solid-phase DNase I footprinting
Selection of phage antibodies by binding affinity. Mimicking affinity maturation. 1992 J Mol Biol Phage display
Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain 1997 Nat Biotechnol Phage display
Immunoseparation of membrane peptidases from pig lung membranes using magnetic beads 1994 Biochem Soc Trans Isolation and immobilization of active enzymes
A casein kinase I isoform is required for proper cell cycle progression in the fertilized mouse oocyte 1997 J Cell Sci Isolation and immobilization of active enzymes
Mechanisms of DNA demethylation in chicken embryos. Purification and properties of a 5-methylcytosine-DNA glycosylase 1995 J Biol Chem Isolation and immobilization of active enzymes
Differential activation of p70 and p85 S6 kinase isoforms during cardiac hypertrophy in the adult mammal 1998 J Biol Chem Isolation and immobilization of active enzymes
A kinase-cyclin pair in the RNA polymerase II holoenzyme 1995 Nature Isolation and immobilization of active enzymes
Identification of a novel Stat3 recruitment and activation motif within the granulocyte colony-stimulating factor receptor 1999 Blood IP and co-IP
Analysis and isolation of human transferrin receptor using the OKT-9 monoclonal antibody covalently crosslinked to magnetic beads 1991 Anal Boichem IP and co-IP
Insulin resistance is mediated by a proteolytic fragment of the insulin receptor 1995 J Biol chem IP and co-IP
hnRNP A2/B1 binds specifically to single stranded vertebrate telomeric repeat TTAGGGn 1992 Nucleic Acids Res IP and co-IP
Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro 1998 Cell IP and co-IP
Engagement of the adhesion receptor CD22 triggers a potent stimulatory signal for B cells and blocking CD22/CD22L interactions impairs T-cell proliferation 1996 Blood IP and co-IP
Effect of ligand conformation on melanoma cell alpha3beta1 integrin-mediated signal transduction events: implications for a collagen structural modulation mechanism of tumor cell invasion. 1998 Biochemistry Isolation via coupled peptides
Inhibition of melanoma cell binding to type IV collagen by analogs of cell adhesion regulator 1997 J Med Chem Isolation via coupled peptides
Inhibition of anchorage-dependent cell spreading triggers apoptosis in cultured human endothelial cells 1994 J Cell Biol Isolation via coupled peptides
Clustering of vitronectin and RGD peptides on microspheres leads to engagement of integrins on the luminal aspect of endothelial cell membrane 1994 Blood Isolation via coupled peptides
Multicolor in vitro translation 2003 Nat Biotechnol Isolation via coupled peptides
Duck lymphocytes. VII. Selection of subpopulations using lectin-coated magnetic beads. 1995 Vet Immunol Immunopathol Isolation via coupled carbohydrates
Selection of carbohydrate-binding cell phenotypes using oligosaccharide-coated magnetic particles 1997 Glycobiology Isolation via coupled carbohydrates
Human granulocytes express a 55-kDa lipopolysaccharide-binding protein on the cell surface that is identical to the bactericidal/permeability-increasing protein. 1993 J Immunol Isolation via coupled carbohydrates
A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1999 J Mol Biol Phage display
Biopanning phage display libraries using magnetic beads vs. polystyrene plates 1999 Biotechniques Phage display
Retroviral vectors displaying functional antibody fragments 1993 Nucleic Acids Res Phage display
Methodology for selection of human antibodies to membrane proteins from a phage-display library 1997 J Immunol Methods Phage display
Cloning of anti-Gal Fabs from combinatorial phage display libraries: structural analysis and comparison of Fab expression in pComb3H and pComb8 phage 1997 Mol Immunol Phage display
Immunomagnetic separation of Salmonella from foods and their detection using immunomagnetic particle (IMP)-ELISA 1995 Int J Food Microbiol Bio- and immunoassays
Characterization of a hypoxia-responsive adhesion molecule for leukocytes on human endothelial cells 1995 J Immunol Bio- and immunoassays
Application of magnetic beads in bioassays 1993 Biotechnology (N Y) Bio- and immunoassays
A simple and sensitive bioassay for the detection of IL-2 activity 1988 J Immunol Methods Bio- and immunoassays
A specific in vitro bioassay for measuring erythropoietin levels in human serum and plasma 1990 Blood Bio- and immunoassays
Unique murine tumor-associated antigens identified by tumor infiltrating lymphocytes 1990 J Immunol Bio- and immunoassays
An improved negative immunomagnetic selection strategy for the purification of primitive hemopoietic cells from normal bone marrow 1991 Exp Hematol Bio- and immunoassays
Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins 1997 Infect Immun Bio- and immunoassays
Immunomagnetic purification of human microvessel endothelial cells using Dynabeads coated with monoclonal antibodies to PECAM-1 1993 Eur J Cell Biol Bio- and immunoassays
Antiviral effect and ex vivo CD4+ T cell proliferation in HIV-positive patients as a result of CD28 costimulation 1996 Science Bio- and immunoassays
Immunomagnetic isolation of islets from the rat pancreas 1997 Biotechnol Prog Bio- and immunoassays
Immunobead filtration: a novel approach for the isolation and propagation of tumor cells 1997 Am J Pathol Bio- and immunoassays
Removal of HIV antigens and HIV-infected cells in vitro using immunomagnetic beads 1990 J Virol Methods Bio- and immunoassays
Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples 1997 Appl Environ Microbiol Isolation of bacteria
Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction 1992 J Clin Microbiol Isolation of bacteria
Detection of Campylobacter jejuni in food and poultry viscera using immunomagnetic separation and microtitre hybridization 1997 J Appl Microbiol Isolation of bacteria
Development of the immunomagnetic enrichment method selective for Vibrio parahaemolyticus serotype K and its application to food poisoning study 1992 Appl Environ Microbiol Isolation of bacteria