Invitrogen DynaGreen™ magnetic beads is a highly magnetic, submicron bead platform with a pioneering greener design from manufacture to customer site. Building on the strong heritage within the realm of biomolecule capture, the team behind Dynabeads has applied more than 30 years of experience with the 12 principles of green chemistry to create high performing, non-microplastic magnetic beads that give reproducible results with low non-specific binding. The choice is a better option for the environment and in line with greener lab policies, without compromising on the quality of results. Leading with DynaGreen for immunoprecipitation, these are the first products in a growing greener DynaGreen magnetic bead lineup.
DynaGreen has sprung from a desire and a need to move towards more sustainable research laboratories. 12 principles of green chemistry were applied at each development step, leading to a product that’s good for your work and good for the environment. Here is how DynaGreen magnetic beads are contributing to more sustainable science:
Immunoprecipitation (IP) is the affinity purification of proteins, protein complexes, protein-nucleic acid complexes, and other antigens (Ag) using a specific antibody that is immobilized to a solid support such as magnetic beads. DynaGreen magnetic beads for the Immunoprecipitation workflow are 250-micron superparamagnetic beads that enable high performance direct and indirect immunoprecipitation (IP) and are ideally suited for applications where yield and purity are of utmost importance, such as mass spectrometry.
The sub-micron bead size of DynaGreen magnetic beads increases the available target capture surface area and provides a low sedimentation rate resulting in efficient isolation of target protein in a simple bind-incubate-elute immunoprecipitation protocol that does not require pre-clearing. The magnetic separation technology used is also rapid and gentle, thereby causing minimal physical stress to your target proteins. The entire protocol can be automated on the KingFisher purification systems to reduce the processing time to 40 minutes.
The principle of isolation is easy; a target protein specific antibody (Ab) is incubated with DynaGreen magnetic beads followed by a wash to remove unbound antibodies. The Ab-bound beads are then incubated with the protein containing sample for capture, followed by washing and elution.
Antibodies are added directly to the sample and incubated to bind their target. After a short incubation, DynaGreen beads are added to the sample containing proteins and antibodies. The beads will bind the antibody, successfully isolating antibody-protein complex, followed by washing and elution.
|When to use||Particularly well suited when you are using rabbit antibody.|
Suitable when you want to pull down large amounts of protein using a rabbit antibody.
|Particularly well suited when you are using mouse and rabbit antibodies.|
A great all-round bead for when you want high yields of target protein with mouse and rabbit antibodies.
Suitable for all types of uses and works well with mass spectrometry.
|When a high purity of sample is required, particularly for applications such as mass spectrometry.|
With secondary antibodies from multiple species.
|Binding capacity||13–14 mg/mL|
|Quantity beads per IP||25 µl*|
|Protocols||Direct and indirect immunoprecipitation|
|Mass spec compatible||Yes|
|Automation||Automation ready—protocols available for KingFisher Sample Purification Systems|
|Recyclable primary packaging||Yes—recycling instructions provided digitally with pack insert|
|Sustainability||ACT label third-party verification of the sustainable impacts of a product, its manufacturing operations, and its end of life|
* High binding capacity means that a smaller amount of beads are required per IP. Competitors normally use 40–100 µl.
Mass spectrometry is a powerful tool for identification, quantification and characterization of proteins in complex biological samples. A standard proteomics workflow includes sample extraction, protein isolation and digestion, HPLC separation and analysis of the resulting peptides with tandem mass spectrometry. DynaGreen magnetic beads are suitable for mass spectrometry.
Enrichment can be easily executed through both direct and indirect IP with DynaGreen magnetic beads. In Figures 1A and 1B, we show that high-quality pulldown of both high-abundance (CD3) and low-abundance (CD81) target protein is easily achieved with DynaGreen magnetic beads at comparable or better performance than today’s standard.
Figure 1A and 1B. Comparable or better performance with DynaGreen magnetic beads.
Equal amounts of sample material with respect to Ab and cell lysate were used for all IP protocols according to the manufacturer’s protocol. We found the following:
DynaGreen products have been designed with automation in mind and scaling your experiments has never been easier. Seamlessly perform your direct or indirect immunoprecipitation on our KingFisher automation systems and bring your experiment time down to 40 min with up to 96 samples per run. Suitable for medium- to high-throughput laboratories, the KingFisher automation systems significantly reduce hands-on time while maintaining the same high target yield and low non-specific binding as the manual protocol.
For Research Use Only. Not for use in diagnostic procedures.