Improve your bioanalysis workflow

Overcome the challenges associated with traditional affinity purification methods using Thermo Scientific™ MSIA™ (Mass Spectrometric Immunoassay) microcolumns. Designed for analytical affinity purification, ready-to-use MSIA microcolumns are coated with target-specific affinity ligand to capture low abundant target analytes from even the most complex biological samples. Secure resourceful innovation that enables rapid assay development, provides high specificity and reduces variability to have data that you can trust.

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What is MSIA?


Mass Spectrometric Immunoassay (MSIA) microcolumns are coated with affinity ligands giving you the power to capture even low abundant analytes from the most complex samples. MSIA can be used on low and high throughput formats and help to enable simple, reproducible and precise affinity capture-- suitable for your bioanalysis workflow.


Bioanalysis Innovation
tailored to you
Ready-to-use
affinity ligand coated microcolumns
Capture
analytes with speed, precision & confidence 
Elute
reliable results for
increased data confidence 

MSIA microcolumns can be used on both automated high throughput and semi-automated low throughput formats, enabling simple, precise affinity capture that fits your biomolecule analysis workflow.

MSIA microcolumns are available for:

Housed within pipette tips, MSIA microcolumns are coated with target specific affinity ligand providing highly specific affinity capture. 

MSIA microcolumns capture even the low abundant analytes with superior quantitation performance, down to the femtomole level.

Cleaner analyte of interest enables low background, high senstivity and enhanced reproducibility.

Empower your biomolecule analysis workflow with MSIA microcolumns - providing data you can trust.

 

MSIA vs. Traditional Methods


Select the best MSIA product for your application

 

MSIA D.A.R.T.’S, Streptavidin

MSIA
Streptavidin-
EVO

MSIA D.A.R.T.’S, Insulin 

*MSIA
D.A.R.T.’S, Custom


*MSIA D.A.R.T.’S,
Protein A, Protein G & Protein A/G 

Description

Based on the Streptavidin-biotin chemistry.

Enable a broad array of MSIA applications.
Based on the Streptavidin-biotin chemistry.

Enable a broad array of MSIA applications.
For purification of various insulin analogs, including endogenous human insulin, therapeutic analogs and non-human animal analogs. Allows direct 
linking of **affinity ligand of your choice to MSIA microcolumn.
A versatile option to bind and orientate a wide range of antibodies, enabling flexibility to use antibodies of choice specific to the application .

Low
Throughput
 

Finnpipette
Novus i
Multichannel Electronic Pipette
- - - -  Finnpipette
Novus i Multichannel Electronic Pipette
Finnpipette
Novus i Multichannel Electronic Pipette
Finnpipette
Novus i Multichannel Electronic Pipette

High
Throughput

Thermo Scientific
Versette
Automated Liquid Handler 
Tecan’s Freedom EVO Platform
with MCA96 liquid handling arm
Thermo Scientific Versette Automated Liquid Handler Thermo Scientific Versette
Automated Liquid Handler
Thermo Scientific Versette Automated Liquid Handler 

Binding 
Capacity

Immobilized Stretavidin: 4.0 µg/microcolumn (<15%)

Bindingcapacity:
4 µg/microcolumn (<15%) [based on biotinylated-IgG]

Immobilized Stretavidin: 10 µg/microcolumn (<15%)

Binding capacity:
7.5 µg/microcolumn (<15%) [based on biotinylated-IgG]

N/A N/A Protein A: 4 µg IgG/microcolumn

Protein G: 4 µg IgG/microcolumn

Protein A/G: 4 µg IgG/microcolumn

Application

For microscale purification of
target protein, peptide or antibody using target specific biotin conjugated affinity ligands. 
For microscale purification of target protein, peptide or antibody using target specificbiotin conjugated affinity ligands. Simultaneous quantitative and qualitative bioanalysis of insulin including endogenous and therapeutic
analogs.
Tailored to capture
& elute protein, peptide or antibody of your interest.
Allows use of untagged
antibodies for
your specific applications.

Relevant
Industries

Biopharma

CRO

Sports anti-doping

Translational research

Biopharma

CRO

Sports anti-doping

Translational research

Biopharma

CRO

Forensic

Sports anti-doping

Biopharma

Clinical/Translational

CRO

Sports anti-doping

Biopharma

CRO

Sports anti-doping

Translational research

*Direct linking of affinity ligand for the target analyte to the microcolumn.
**Affinity ligand must meet specific selection criteria. Either provide the affinity ligand or request us to directly buy from the vendor.

MSIA products

MSIA™ D.A.R.T.'S™ for the Versette and Novus i Platform

Enabled through proprietary MSIA D.A.R.T.'S™ technologies, these microcolumns have demonstrated pg/mL level detection while providing simultaneous qualitative/quantitative data content from complex biological matrix, such as human plasma.

MSIA™ Microcolumns for the Tecan Freedom EVO Platform

MSIA affinity micro columns, designed for Freedom EVO platform (MCA 96 head), provide innovative enablement in the areas ranging from bioanalysis of therapeutic mAbs, antibody-drug conjugates (ADC), biotransformation [in-vivo modifications, drug-antibody ratio (DAR)], preclinical and clinical drug trials (PKPD, DMPK, and ADME) to biomarker and proteins analysis. Learn more

Finnpipette™ Novus i Multichannel Electronic Pipette

Overcome the challenges associated with traditional affinity purification methods using Thermo Scientific™ MSIA™ (Mass Spectrometric Immunoassay) microcolumns. Designed for analytical affinity purification, ready-to-use MSIA microcolumns are coated with target-specific affinity ligand to capture even low abundant target analytes from the most complex biological samples. Secure resourceful innovation that enables rapid assay development, provides high specificity and reduces variability to have data that you can trust.

Equipment and instruments related to MSIA microcolumns

Versette™ Pipetting Workstation

Support a wide range of applications with the Thermo Scientific™ Versette™ Automated Liquid Handler, a compact handler featuring user-friendly programming, reliable performance and a choice of 96- or 384-channel pipetting heads.

Vanquish™ Flex Binary UHPLC System

Obtain new benchmarks in accuracy, precision, and sensitivity. The Thermo Scientific™ Vanquish™ Flex Binary UHPLC system is biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending. Learn more ›

Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer

The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology. Learn more

Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer

The Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer takes Quanfirmation to the next level with increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies. Learn more

Capture the potential

Additional resources

Find more information regarding MSIA, including: videos, brochures, application notes, protocol examples, publications and webinars in our resource library.

Visit the MSIA Resource Library ›