Novex Assay Kits for Luminex Assays

Luminex assays help save time, money and sample

Invitrogen™ multiplex assays for the Luminex™ platform enable simultaneous measurement of multiple proteins per well using as little as 25 μL of sample. This technology produces results comparable to ELISA but with greater efficiency, speed, and dynamic range. Plus, multiplexing is less expensive per target than ELISA. With our flexible formats you can purchase premixed panels, mix your own singleplex kits or have us mix it for you through our custom services.

We also offer complimentary in-person consulting through our field application specialists (FAS). Our team of specialist will answer your technical question, help you choose an assay, troubleshoot and more—face to face or on the phone, at no cost. Contact us at for personalized assistance.

Download our guide


Find out about the latest tools available for protein detection and quantitation. Download our free immunoassay guide to protein quantitiation

Need help?

We offer complimentary technical consulting with a field application specialist. Contact us

Popular products

Cytokine 30-Plex Panel, Human Magnetic Luminex Assay
Cytokine 20-Plex Panel, Mouse Magnetic Luminex Assay
Magnetic 96-Well Separator for Luminex Assays

Featured categories

Multiplex assays for the Luminex platform

Assay kits using magnetic and nonmagnetic polystyrene beads for simultaneous measurement of 3 to 30 analytes (cytokines, growth factors, etc.) using Invitrogen™ Luminex xMAP™ (multi-analyte profiling) technology.

Singleplex assays for the Luminex platform

Reagent sets of antibody-coated polystyrene (non-magnetic) beads, detector antibody, standards for measuring individual analytes using Luminex xMAP technology. Create and validate your own multiplexed combinations.

Custom assays for the Luminex platform

Select from a large portfolio of available targets to design your own multiplex assay and allow us to blend, optimize, and test it for you for use with Luminex 100/200™, FLEXMAP 3D™, and MAGPIX™ systems.

Magnetic singleplex assays for the Luminex platform

Reagent sets of antibody-coated magnetic beads, detector antibody, standards for measuring individual analytes using Luminex xMAP technology. Create and validate your own multiplexed combinations.

Singleplex, multiplex and custom assays for the Luminex platform

Our Invitrogen assays, available in polystyrene and magnetic bead formats, are an integral part of cytokine, pathway analysis, and biomarker discovery screening workflows.

  • Singleplex assays can be used to analyze individual protein targets—mix your own custom panels or try premixed multiplex assays
  • Premixed multiplex assays are available for the most widely-studied cytokines, adhesion proteins, and growth factors for human, primate (monkey), mouse, rat, and swine research samples
  • Custom luminex assays from a range of available protein targets, blended for you by our team to optimize for low background, high signal-to-noise ratios, and linearity of dilution

Our Invitrogen assays for the Luminex platform are easy to set up and run, using a procedure that is very similar to an ELISA (Figure 1). If you are new to Luminex xMAP technology or are using assays for the Luminex platform for the first time, we have a team of dedicated Field Application Scientists (FASs) ready to help. Our FASs come to your lab to guide your efforts running Luminex assays and using Luminex instruments and software. Contact  to request your personal consultation today.

Luminex xMAP technology is based on polystyrene and paramagnetic microspheres (beads) that are internally dyed with red and infrared fluorophores of differing intensities.  Each dyed bead is given a unique number, or bead region, allowing the differentiation of individual beads.  Next, individual bead sets are coated with a capture antibody qualified for a specific analyte. Multiple analyte-specific beads can then be combined in a single well of a 96-well microplate assay format to detect and quantify up to 100 targets simultaneously using a Luminexinstrument for analysis.

Tables 1, 2, and 3 provide examples of the results achieved with our Invitrogen assays and protein standards (included in the assays). Unlike other assays, we use the maximum number of beads in each assay, so that data are collected from at least 100 events per bead region per target. Measurement of a minimum of 100 events, compared to the 50 recommended by some manufacturers, enables lower %CVs and more precise results.

Our Invitrogen assays are subject to rigorous quality control and validation prior to release; we extensively test cross-reactivity to help ensure the specificity of each assay for its intended target. In addition, the protein standards we supply are precisely calibrated to reference materials for maximum consistency.

Figure 1. Fast and easy Invitrogen assay protocol. Beads of defined spectral properties are conjugated to protein-specific capture antibodies and added along with samples (including standards of known protein concentration, control samples, and test samples), into the wells of a filter-bottom microplate. Target protein binds to the capture antibodies over the course of a two hour incubation. After washing the beads, protein-specific, biotinylated detector antibodies are added and incubated with the beads for 1 hr. Next, excess biotinylated detector antibodies are removed, and streptavidin-conjugated fluorescent protein, R-Phycoerythrin (SAV-R-PE), is added and incubated for 30 min. SAV-R-PE binds to the biotinylated detector antibodies, forming a four-member, solid-phase sandwich. After washing to remove unbound SAV-RPE, the beads are analyzed with a Luminex detection system. By monitoring the spectral properties of the beads and the amount of associated R-Phycoerythrin (R-PE) fluorescence, the concentration of one or more proteins can be determined.

Table 1. More reliable data. These data indicate that %CVs and assay precision are improved by collecting data from 100 bead events compared to collecting data from only 50 bead events.

Sample Description
50 Bead events
(0.5X Beads/ well)
100 Bead events
(1X Beads/ well)
Average (Serum)8.712.0
Average (Plasma)14.8 7.3
Average (TCS)8.55.2
Average (All)10.56.9

Table 2. Better-calibrated standards. Our Invitrogen assays are calibrated to National Institute of Biological Standards and Controls (NIBSC), a set of international reference standards, when available, or to published intracellular cell models for phosphoprotein activity to help ensure that measurements are relevant. Protein standards are tested before and after lyophilization, in single as well as multiplexed assays, for consistency in standard integrity and performance.

Standards for Invitrogen multiplex assays for the Luminex platform


Competitor Standards


Table 3. Greater assay specificity. We measured assay response from dozens of like-target proteins to help ensure that the antibodies selected for use in our Invitrogen assays are highly specific for their desired targets. In this table, we compare results (fluorescence units shown) for similar target proteins using our Invitrogen assays or assays from another manufacturer. None of the Invitrogen assays showed significant signal from similar, non-target proteins, whereas a few of the assays from the other supplier showed significant cross-reactivity with non-target proteins.

Invitrogen multiplex assays for the Luminex platform

  IL-6  IL-10  TNF-

Competitor assay fluorescence units

  IL-6  IL-10  TNF-