Novex Assay Kits for Multiplex Luminex Assays

Multiplex Luminex® assays enable the simultaneous analysis of multiple proteins in single samples from a broad range of biological sources. Based on Luminex® xMAP® (multi-analyte profiling) technology, Invitrogen™ multiplex assays combine the efficiency of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA. We make Luminex® xMAP® technology simple and reliable to use by providing both nonmagnetic (polystyrene) and magnetic kits, which include ready-to-use validated combinations of bead-coated and detector antibodies, microplates and other reagents.

Popular products

Cytokine 30-Plex Panel, Human Magnetic Luminex Assay
Cytokine 29-Plex Panel, Monkey Magnetic Luminex Assay
Cytokine 20-Plex Panel, Mouse Magnetic Luminex Assay

Features of Invitrogen multiplex kits for Luminex® assays

Our multiplex kits undergo rigorous quality testing and are calibrated against matching Invitrogen™ ELISA kits where available (Table 1) to show comparable analytical results between protein analysis platforms.

  • Fast and efficient—simultaneous analysis of up to 100 proteins using only 50 μL
  • Accessible—a broad menu of Invitrogen immunoassay kits based on xMAP® technology
  • Economical—helps reduce time and costs compared to running multiple western blots and ELISA assays
  • High specificity—lower cross-reactivity with other targets (Table 2)

Figure 1. Strong correlation of ELISA and Invitrogen multiplex assay results. Mouse GM-CSF in tissue culture supernatant was tested by ELISA and in an Invitrogen™ (Novex™) multiplex assay for Luminex platform. Correlation of values over 3 orders of magnitude of sample dilution was 0.9868.


Table 1. Rigorous assay validation of Invitrogen multiplex kits helps ensure consistent, reliable results.

Specification Description
Benchmarking to ELISA
(Figure 1)
Correlates with ELISA data (>90% correlation)
Recovery Tested on serum and plasma
Sensitivity* Physiologically relevant levels, <10 pg/mL (based on detectable signal >2 SD above background)
Precision (Figure 2) Inter-assay CV: <10%
Intra-assay CV: <10%
Specificity Cross-reactivity tests are performed with other analytes and antibodies
Linearity of dilution High coefficient of correlation between sample dilutions and expected concentration over the range of the assay
Parallelism to natural samples (Figure 3) (R2 > 0.99) Recombinant standards are compared to natural samples to ensure equivalency
* Every kit has its own specifications. Please consult the protocol insert in your specific Novex® multiplex kit.

PB4410_Multiplex_Luminex_Assays_figure2

Figure 2. Assay precision. Orange and gray bars each represent 24 replicates measured on separate days. CVs in all cases were <10%. Data were generated using a Invitrogen™ Human Cytokine Magnetic 10-Plex Panel (Cat. No. LHC0001M).


PB4410_Multiplex_Luminex_Assays_figure3
PB4410_Multiplex_Luminex_Assays_figure4

Figure 3. Parallelism to natural samples. To evaluate Invitrogen™ (Novex™) multiplex assays, samples of 23 human protein markers were spiked into a sample of human serum, and the sample was processed using the manufacturer’s instructions for the Invitrogen multiplex assay kit and using similar kits from two other suppliers. The multiplex sample was quantified on the MAGPIX® system, and percent recovery calculated for (A) the individual markers and (B) as an average for the entire group.


Table 2. Specificity data from the Human Cytokine 10-Plex Panel (Cat. No. LHC0001) show no significant cross-reactivity.

Numbers represent mean fluorescence intensity (MFI) units generated when individual recombinant proteins were analyzed independently in a series of 10-plex assays.

Antibody bead kit
IL-1β› IL-2 IL-4 IL-5 IL-6 IL-8 IL-10 TNF-០IFN-γ GM-CSF
IL-1β› 6,332 41 37 13 9 37 32 27 18 51
IL-2 38 11,324 47 17 26 40 33 27 15 50
IL-4 38 41 10,503 15 25 40 32 25 12 50
IL-5 48 54 36 8,265 11 34 33 25 16 51
IL-6 44 72 40 11 6,827 42 31 22 12 70
IL-8 39 39 44 12 27 10,002 32 27 19 52
IL-10 41 51 39 11 12 41 8,151 23 12 46
TNF-០32 55 36 10 7 60 30 6,642 10 47
IFN-γ 32 41 39 10 8 70 35 25 3,540 51
GM-CSF 41 40 36 12 8 46 33 25 13 4,932
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