Profile more biomarkers with less sample

Invitrogen ProcartaPlex multiplex immunoassays use the Luminex xMAP (multianalyte profiling) technology that enables the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. The technology uses differentially dyed capture beads for each target in a multiplex “ELISA-like” assay. ProcartaPlex immunoassays can profile up to 80 times more analytes using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex immunoassays provide the ability to create a unique panel with the assurance to achieve reproducible results throughout the course of your study.


Features of ProcartaPlex assays
  • Magnetic bead format for increased ease-of-use and throughput
  • More than 90% of ProcartaPlex assays can be combined with one another
  • High correlation to our ELISA assays (R2 >0.9)
  • Scalable and reproducible performance regardless of plex size
  • Largest published panel for quantitation of up to 65 analytes

Download the guide


ProcartaPlex multiplex panels

Multiplex panels are pre-configured biologically-defined and disease-defined panels using magnetic beads for quantitative multiplex analysis of multiple targets in a single sample. ProcartaPlex immunoassays are available in multiple formats across six species (human, mouse, rat, non-human primate, porcine, and canine).

View full listing of multiplex panels

Panel features

  • Choose from a variety of commonly investigated biologically- and disease-defined analyte panels.
  • Quantitatively measure secreted biomarkers in plasma, serum, other bodily fluids, and cell culture supernatants.
  • Select “convenience” panels to use the assay as configured, or select “configurable” panels to customize the assay by combining with another panel or adding compatible ProcartaPlex simplex beads.
  • Alternatively, simplex kits  can be purchased individually with a ProcartaPlex basic Kit (includes all non-analyte specific reagents needed to run the ProcartaPlex assay) to create your unique customized multiplex panel.
  • Selected panels are available in "Platinum" or "High-Sensitivity" formats (see sections that follow).

Human combinable panels

Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.

Human Panel Name Cat. No. Antigens
Cytokine/Chemokine/Growth Factor 45-Plex Human Panel 1 EPX450-12171-901 BDNF, EGF, Eotaxin, FGF-basic, GM-CSF, GROα, HGF, IFNγ, IFNα, IL-1RA, IL-1β, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1α, MIP-1β, NGFβ, PDGF-BB, PLGF, RANTES, SCF, SDF1α, TNFα, TNFβ, VEGF-A, VEGF-D
Cytokine & Chemokine 34-Plex Human Panel 1A EPX340-12167-901 Eotaxin, GM-CSF, GROα, IFNα, IFNγ, IL-1β, IL-1α, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFα, TNFβ
Cytokine 25-Plex Human Panel 1B EPX250-12166-901 GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, TNFα, TNFβ
Inflammation 20-Plex Human Panel EPX200-12185-901 E-Selectin, GM-CSF, ICAM-1, IFNα, IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IP-10, MCP-1, MIP-1α, MIP-1β, P-Selectin, TNFα
Th1/Th2 & Chemokine 20-Plex Human Panel 1 EPX200-12173-901 Eotaxin, GM-CSF, GROα, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-12 p70, IL-13, IL-18, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFα
Th1/Th2 & Cytokine 18-Plex Human Panel 1C EPX180-12172-901 GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-12 p70, IL-13, IL-15, IL-18, IL-31, TNFα, TNFβ
Th1/Th2/Th9/Th17/Th22/Treg Cytokine 18-Plex Human Panel EPX180-12165-901 GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, TNFα
Th9/Th17/Th22/Treg & Chemokine 16-Plex Human Panel 1 EPX160-12175-901 Eotaxin, GROα, IL-8, IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27,IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α
Cytokine 1C & Chemokine 16-Plex Human Panel 1 EPX160-12176-901 Eotaxin, GROα, IFNα, IL-1α, IL-1RA, IL-7, IL-8, IL-15, IL-31, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFβ
Th9/Th17/Th22/Treg & Cytokine 14-Plex Human Panel 1C EPX140-12174-901 Eotaxin, GROα, IL-8, IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27,IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α
Immuno-Oncology Checkpoint 14-Plex Human Panel EPX14A-15803-901 BTLA, CD28, CD80, CD137, CD27, CD152, GITR, HVEM, IDO, LAG-3, PD-1, PD-L1, PD-L2, TIM-3
Growth Factor 11-Plex Human Panel EPX110-12170-901 BDNF,NGFβ, EGF, FGF-basic, HGF, LIF, PDGF-BB, PlGF, SCF, VEGF-A, VEGF-D
Th1/Th2 Cytokine 11-Plex Human Panel EPX110-10810-901 GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12 p70, IL-13, IL-18, TNFα
Chemokine 9-Plex Human Panel 1 EPX090-12187-901 Eotaxin, GROα, RANTES, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, SDF1α
Th9/Th17/Th22/Treg Cytokine 7-Plex Human Panel EPX070-10817-901 IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27
Cytokine 7-Plex Human Panel 1C EPX070-10010-901 IFNα, IL-1α, IL-1RA, IL-7, IL-15, IL-31, TNFβ
Essential Th1/Th2 Cytokine 6-Plex Human Panel EPX060-10009-901 IFNγ, IL-4, IL-5, IL-6, IL-12 p70, TNFα
MMP 5-Plex Human Panel 1 EPX050-10015-901 MMP-1, MMP-7, MMP-8, MMP-12, MMP-13
Essential 4-Plex Human Panel 2 EPX040-10008-901 IL-8, IL-1β, IL-10, IL-17A
MMP 3-Plex Human Panel 2 EPX030-10829-901 MMP-2, MMP-3, MMP-9

Human convenience panels

Beads and detection antibodies are premixed in one vial each. Panels are not combinable with other ProcartaPlex assays.

Human Panel Name Cat. No. Antigens
Immune Monitoring 65-Plex Human Panel EPX650-10065-901 APRIL, BAFF, BLC, CD30, CD40L, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, FGF-2, Fractalkine, G-CSF, GM-CSF, Gro a, HGF, IFN-a, IFN-g, IL-10, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-18, IL-1a,IL-1b, IL-2, IL-20, IL-21, IL-22, IL-23, IL-27, IL-2R, IL-3, IL-31, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IP-10, I-TAC, LIF, MCP-1, MCP-2, MCP-3, M-CSF, MDC, MIF, MIG, MIP1a, MIP-1b, MIP-3a, MMP-1,NGF beta, SCF, SDF-1a, TNF b, TNF-a, TNF-R2, TRAIL, TSLP, TWEAK, VEGF-A
Cytokine/Chemokine/Growth Factor Convenience 45-Plex Human Panel 1 EPXR450-12171-901 BDNF, EGF, Eotaxin, FGF-basic, GM-CSF, GROα, HGF, IFNγ, IFNα, IL-1RA, IL-1β, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1α, MIP-1β, NGFβ, PDGF-BB, PLGF, RANTES, SCF, SDF1α, TNFα, TNFβ, VEGF-A, VEGF-D
Cytokine & Chemokine Convenience 34-Plex Human Panel 1A EPXR340-12167-901 Eotaxin, GM-CSF, GROα, IFNα, IFNγ, IL-1β, IL-1α, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, SDF1α, TNFα, TNFβ
TNF Superfamily 15-Plex Human Panel EPX150-15801-901 4-1BBL, APRIL, BAFF, CD30L, EDA-1, FasL, GITRL, CD40L, NGFβ, TNFα, TNFβ, LIGHT, RANKL, TRAIL, TWEAK
TNF Receptor Superfamily 12-Plex Human Panel EPX120-15802-901 Fas (APO), CD27, CD30, CD40, 4-1BB , DR6, GITR, HVEM, TNF-R1, TNF-R2, TRAIL-R1,TRAIL-R2
Metabolism/Obesity 9-Plex Human Panel 2 EPX09A-15804-901 C-peptide, Cortisol, FGF-23, GLP-1, Ghrelin, Insulin, PYY, Leptin, Resisitin
Graft Versus Host Disease 8-Plex Human Panel EPX080-15814-901 CD30, Granzyme A, HGF, IL-2R (CD25), KRT18, ST2/IL33R, Syndecan, TNF RI
Myokine 8-Plex Human Panel EPX080-12186-901 BDNF, IL-1RA, IL-10, IL-15, IL-6, IL-8, LIF, TNFα
Antibody Isotyping 7-Plex Human Panel EPX070-10818-901 IgG1, IgG2, IgG3, IgG4, IgE, IgA, IgM
Coagulation 6-Plex Human Panel 1 EPX060-10824-901 Factor XI, Factor XII, Factor XIII, Antithrombin, Prothrombin, CRP
Metabolism/Obesity 5-Plex Human Panel 1 EPX050-15805-901 Adiponectin, ICAM-1, NGAL, ZAG, RBP4
Coagulation 4-Plex Human Panel 3 EPX040-10825-901 Factor IX, Protein S, Protein C, vWF
Coagulation 3-Plex Human Panel 2 EPX030-10823-901 Factor V, Factor VII, Factor VIII

Mouse combinable panels

Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.

Mouse Panel Name Cat. No. Antigens
Cytokine & Chemokine 36-Plex Mouse Panel 1A EPX360-26092-901 ENA-78, Eotaxin, G-CSF, GM-CSF, GROα, IFNα, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-15/IL-15R, IL-17A, IL-18, IL-22, IL-23, IL-27, IL-28, IL-31, IP-10, LIF, MCP-1, M-CSF, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα
Cytokine & Chemokine 26-Plex Mouse Panel 1 EPX260-26088-901 Eotaxin, GM-CSF, GROα, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα
Th1/Th2 & Chemokine 20-Plex Mouse Panel 1 EPX200-26090-901 Eotaxin, GROα, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12 p70, IL-13, IL-18, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα
Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse Panel EPX170-26087-901 GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, TNFα
Th9/Th17/Th22/Treg & Chemokine 15-Plex Mouse Panel EPX150-26089-901 IL-10, IL-17A, IL-22, IL-23, IL-27, IL-9, GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, Eotaxin
Th1/Th2 Cytokine 11-Plex Mouse Panel EPX110-20820-901 GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12 p70, IL-13, IL-18, TNFα
Cytokine 10-Plex Mouse Panel 1B EPX100-26091-901 ENA-78, G-CSF, IFNα, IL-1α, IL-3, IL-15/IL-15R, IL-28, IL-31, LIF, M-CSF
Chemokine 9-Plex Mouse Panel 1 EPX090-20821-901 GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, Eotaxin
Essential 8-Plex Mouse Panel 2 EPX080-20832-901 IL-1β, IL-10, IL-17A, GROα, MCP-1, MIP-2, MIP-1α, MIP-1β
Th9/Th17/Th22/Treg Cytokine 6-Plex Mouse Panel EPX060-20822-901 IL-9, IL-10, IL-17A, IL-22, IL-23, IL-27
Essential Th1/Th2 Cytokine 6-Plex Mouse Panel EPX060-20831-901 IFNγ, IL-4, IL-5, IL-6, IL-12 p70, TNFα

Mouse convenience panels

Beads and detection antibodies are premixed in one vial each. Panels are not combinable with other ProcartaPlex assays.

Mouse Panel Name Cat. No. Antigens
Cytokine & Chemokine Convenience 36-Plex Mouse Panel 1A EPXR360-26092-901 ENA-78, Eotaxin, G-CSF, GM-CSF, GROα, IFNα, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-15/IL-15R, IL-17A, IL-18, IL-22, IL-23, IL-27, IL-28, IL-31, IP-10, LIF, MCP-1, M-CSF, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα
Cytokine & Chemokine Convenience 26-Plex Mouse Panel 1 EPXR260-26088-901 Eotaxin, GM-CSF, GROα, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα
Antibody Isotyping 7-Plex Mouse Panel 2 EPX070-20816-901 IgG1, IgG2b, IgG2c, IgG3, IgA, IgE, IgM
Antibody Isotyping 7-Plex Mouse Panel EPX070-20815-901 IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, IgM
Myokine 5-Plex Mouse Panel EPX050-20833-901 IL-10, IL-15/IL-15R, IL-6, LIF, TNFα
IFN alpha/IFN beta 2-Plex Mouse Panel EPX020-22187-901 IFNα, IFN β
Canine Panel Name Cat. No. Antigens
Cytokine/Chemokine/Growth Factor 11-Plex Canine Panel 1 EPX11A-50511-901 IFNγ, IL-2, IL-6, IL-8, IL-10, IL-12 p40, MCP-1, NGFβ, SCF, TNFα, VEGF-A
Porcine Panel Name Cat. No. Antigens
Cytokine & Chemokine 9-Plex Porcine Panel 1 EPX090-60829-901 IL-1 β, IL-4, IL-6, IL-8, IL-10, IL-12p40, IFNα, IFNγ, TNFα

Rat combinable panels

Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.

Rat Panel Name Cat. No. Antigens
Cytokine & Chemokine 22-Plex Rat Panel EPX220-30122-901 IL-1α, G-CSF, IL-10, IL-17A, IL-1β, IL-6, TNFα, IL-4, GM-CSF, IFNγ, IL-2, IL-5, IL-13, IL-12p70, Eotaxin, GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-2, RANTES
Th Complete 14-Plex Rat Panel EPX140-30120-901 L-1 α, IL-1 β, GM-CSF, G-CSF, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A/CTLA-8, TNFα
Chemokine 8-Plex Rat Panel EPX080-30121-901 GROα, MCP-1, MIP-1α, RANTES, Eotaxin, IP-10, MCP-3, MIP-2

Rat convenience panels

Beads and detection antibodies are premixed in one vial each. Panels are not combinable with other ProcartaPlex Assays.

Rat Panel Name Cat. No. Antigens
Antibody Isotyping 6-Plex Rat Panel EPX060-30123-901 IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM
Kidney Toxicity 5-Plex Rat Panel 1 EPX050-30124-901 KIM-1, osteopontin, TFF3, VEGF-A, calbindin
Kidney Toxicity 5-Plex Rat Panel 2 EPX050-30125-901 Albumin, clusterin, cystatin, NGAL, TIMP-1

Non-human primate combinable panels

Products can be combined with other combinable panels and simplex kits. Detection antibody (50X) needs to be diluted prior usage. Assays may contain more than one vial of beads and detection antibody.

Non-Human Primate Panel Name Cat. No. Antigens
Cytokine/Chemokine/Growth Factor 37-Plex NHP Panel EPX370-40045-901 BDNF, BLC, Eotaxin, FGF-2, G-CSF, GM-CSF, IFNα, IFNγ, IL-1β, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-18, IL-1RA, IL-2, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, NGF β, PDGF-BB, sCD40L, SCF, SDF-1α, TNFα, VEGF-A, VEGF-D
Cytokine & Chemokine 30-Plex NHP Panel EPX300-40044-901 BLC, Eotaxin, G-CSF , GM-CSF, IFNα, IFNγ, IL-1β, IL-10, IL-12p70, IL-13, IL-15, IL-17A /CTLA-8, IL-18, IL-1RA, IL-2, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, sCD40L, SDF-1α, TNFα
Th 14-Plex NHP Panel EPX140-40040-901 IFNγ, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-5, IL-6, TNFα, GM-CSF, IL-18, IL-10, IL-17A, IL-23
Chemokine 10-Plex NHP Panel EPX100-40041-901 IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, Eotaxin, SDF-1α, BLC, I-TAC, MIG
Growth Factor 7-Plex NHP Panel EPX070-40043-901 BDNF, FGF-basic, PDGF-BB, SCF, VEGF-A, VEGF-D, NGFβ
Cytokine 6-Plex NHP Panel 1B EPX060-40042-901 G-CSF, IFNα, IL-1RA, IL-15, IL-7, CD40L

Cross-reactivity chart for NHP ProcartaPlex simplex kits

Cat. No. Analyte Chim-
panzee
Afr. gr.
monkey
Ba-boon Cyno-
molgus
Pigtail
macaque
Rhesus
monkey
Mar-
moset
Squirrel
monkey
EPX01A-42032-901 Adiponectin +++ N.T. + + N.T. ++ - -
EPX01A-42116-901 BDNF  +++ ++ + + + ++ + +
EPX01A-42147-901 BLC/CXCL13 +++ +++ +++ +++ ++ +++ ++ +
EPX01A-40239-901 CD40L +++ + +++ - - +++ +++ +
EPX01A-42120-901 Eotaxin +++ + +++ +++ +++ +++ +++ ++
EPX01A-42074-901 FGF-2 +++ +++ ++ +++ ++ +++ +++ +++
EPX01A-42001-901 G-CSF +++ + +++ +++ +++ +++ ++ +
EPX01A-40283-901 GM-CSF +++ +++ + +++ + + + +
EPX01A-42027-901 Granzyme B +++ - - - - - - -
EPX01A-40201-901 ICAM-1 +++ +++ +++ +++ +++ +++ ++ ++
EPX01A-40216-901 IFN alpha ++ - + ++ - ++ ++ -
EPX01A-40228-901 IFN gamma +++ +++ +++ +++ ++ +++ +++ +++
EPX01A-40224-901 IL-1 beta +++ + +++ +++ ++ +++ ++ ++
EPX01A-40642-901 IL-10 +++ + + + - + ++ +
EPX01A-42090-901 IL-12p40 +++ +++ +++ +++ ++ +++ +++ +++
EPX01A-40238-901 IL-12p70 +++ ++ ++ + + + +++ +++
EPX01A-40231-901 IL-13 +++ + +++ + +/- ++ +/- +/-
EPX01A-42089-901 IL-15 +++ ++ ++ ++ + +++ +++ +
EPX01A-42017-901 IL-17A +++ +++ ++ ++ ++ +++ +++ +
EPX01A-42160-901 IL-17F +++ ++ ++ ++ ++ ++ + +
EPX01A-40267-901 IL-18 +++ +++ ++ ++ ++ ++ ++ +
EPX01A-42080-901 IL-1RA +++ ++ +++ +++ +++ +++ + +/-
EPX01A-40221-901 IL-2 +++ - - - - - + +
EPX01A-42023-901 IL-23 +++ ++ ++ ++ ++ +++ ++ ++
EPX01A-40225-901 IL-4 +++ +++ +++ +++ ++ +++ ++ ++
EPX01A-40278-901 IL-5 +++ ++ +++ ++ + ++ ++ -
EPX01A-40213-901 IL-6 +++ + ++ +++ +++ +++ +/- +/-
EPX01A-40237-901 IL-7 +++ - ++ ++ - +++ +++ +
EPX01A-40204-901 IL-8 +++ ++ +++ +++ +++ +++ +/- +/-
EPX01A-40284-901 IP-10 +++ +/- +/- +/- +/- +/- +/- +/-
EPX01A-42124-901 I-TAC +++ +++ +++ +++ ++ +++ ++ ++
EPX01B-40281-901 MCP-1 +++ +++ +++ +++ +++ +++ +/- +/-
EPX01A-42125-901 MCP-3 + + + + + + +++ +
EPX01A-42127-901 MIF +++ +++ +++ +++ +++ +++ +++ +++
EPX01A-40285-901 MIG +++ + ++ +++ ++ +++ ++ ++
EPX01A-42029-901 MIP-1 alpha +++ +++ +++ +++ ++ +++ ++ +++
EPX01A-42030-901 MIP-1 beta +++ +++ +++ +++ +++ +++ +++ +++
EPX01A-42117-901 NGF beta +++ + ++ - - +++ +++ +++
EPX01A-42033-901 PAI-1 +++ ++ + + + +++ +++ ++
EPX01A-42071-901 PDGF-BB +++ ++ +++ +++ +/- +++ +++ +++
EPX01A-40287-901 RANTES +++ +++ +++ +++ +++ +++ +++ +++
EPX01A-42040-901 Resistin +++ +++ +++ ++ +++ + +++ +/-
EPX01A-42137-901 SCF + N.T. N.T. N.T. N.T. + ++ +
EPX01A-42138-901 SDF-1 alpha +++ +++ +++ +++ +++ +++ + +/-
EPX01A-42139-901 TGF alpha ++ + + + + - - +
EPX01A-40249-901 TGF beta 1 +++ +++ ++ ++ ++ +++ +++ ++
EPX01A-40223-901 TNF alpha +++ ++ +++ +++ +++ +++ +++ +
EPX01A-40202-901 TNF beta +++ ++ ++ +++ ++ +++ - -
EPX01A-42004-901 TRAIL +++ ++ +++ ++ ++ ++ ++ ++
EPX01A-40232-901 VCAM-1 +++ +/- +/- - +/- - - -
EPX01A-40277-901 VEGF A +++ +++ +++ +++ +++ +++ +++ ++
EPX01A-42076-901 VEGF D N.T. N.T. N.T. N.T. N.T. N.T. ++ N.T.

N.T. = Not tested

Simplex bead sets are magnetic bead sets used to detect individual analytes. They are designed to be added to ProcartaPlex panels to increase customization. Alternatively, multiple ProcartaPlex simplex bead sets can be combined and run using the ProcartaPlex Basic Kit that includes all non-target specific reagents needed to perform the ProcartaPlex assay.

View full listing of simplex kits

ProcartaPlex basic kits

Cat. No. Basic Kit Description
EPX010-10420-901 Human Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-40420-901 Non-Human Primate Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-20440-901 Mouse Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-50515-901 Canine Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-60460-901 Porcine Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-30420-901 Rat Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay

Platinum kits and panels are designed to overcome matrix effects from serum and plasma samples, thereby improving spike- and dilution-recovery results. Serum and plasma samples are complex matrixes with many components. This complexity can cause the so called matrix effect which can impact the readout of many cytokines (low spike recovery and the dilution linearity). We developed matrix type specific sample diluents to overcome these matrix and multiplex effects, achieving high performance specifications comparable to those of traditional ELISA. These newly developed surrogate matrices for dilution of serum or plasma samples are included in ProcartaPlex Platinum kits, which produce spike- and dilution-recovery results of 70–130%.

Platinum basic kits

Cat. No. Name Description
EPXP010-10421-901 Platinum Basic Kit for Plasma Samples Includes antigen standard, plasma assay diluent, detection antibody diluent, controls, wash buffers, SA-PE and plates - needed to perform the Platinum ProcartaPlex Simplex assay
EPXP010-10422-901 Platinum Basic Kit for Serum Samples Includes antigen standard, serum assay diluent, detection antibody diluent, controls, wash buffers, SA-PE and plates - needed to perform the Platinum ProcartaPlex Simplex assay

Platinum panels

Cat. No. Name Antigens
EPXP420-10200-901 Platinum ProcartaPlex Panel 1 (42plex) BDNF, Eotaxin (CCL11), GM-CSF,GRO alpha (KC/CXCL1), HGF, IFN alpha, IFN gamma, IL-10, IL-12p70, IL-13, IL-15, IL-16, IL-17A (CTLA-8), IL-1 alpha, IL-1 beta, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-9, IL-20, IL-21, IP-10, LIF, MCP-2, MIP-1 alpha (CCL3),MIP-1 beta (CCL4), Osteoprotegerin (OPG),PDGF-BB, PECAM-1, P-selectin, RANTES (CCL5), SCF, TNF alpha, TNF-RII, tPA, TSLP, VEGF-A, VEGF-D
EPXP130-10100-901 Platinum ProcartaPlex Panel 2 (13plex) IFN gamma, GM-CSF, IL-10, IL-12p70, IL-13, IL-17A (CTLA-8), IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8 (CXCL8), TNF alpha

View full listing of platinum simplex kits

ProcartaPlex high sensitivity assays are designed to measure small concentration differences in cell culture supernatants, plasma, and serum samples with 10-fold lower LLOQs (Lower Limit of Quantification) and sensitivities for all analytes in the femtogram range. Overcome the sensitivity detection limits of conventional multiplex immunoassays.

ProcartaPlex high sensitivity panels

HS Panel Name Cat. No. Antigen
9-Plex Human Panel,
High Sensitivity
EPXS090-12199-901 IFNγ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12 p70, IL-17A, TNFα
5-Plex Mouse Panel,
High Sensitivity
EPXS050-22199-901 IFNγ, IL-2, IL-4, IL-6, TNFα

 

Human high sensitivity simplex kits

Simplex Cat. No. Analyte ULOQ*
(pg/mL)
LLOQ*
(pg/mL)
Inter Assay
%CV
Intra Assay
%CV
EPXS010-10283-901 GM-CSF 7330 1.80 <10 <10
EPXS010-10228-901 IFN gamma 260 0.31 <10 <10
EPXS010-10224-901 IL-1 beta 1330.0 0.32 <10 <10
EPXS010-10221-901 IL-2 490 0.44 <10 <10
EPXS010-10225-901 IL-4 560 0.86 <10 <10
EPXS010-10278-901 IL-5 2720 0.66 <10 <10
EPXS010-10213-901 IL-6 2850 1.03 <10 <10
EPXS010-10204-901 IL-8 890 0.22 <10 <10
EPXS010-10215-901 IL-10 750.0 0.18 <10 <10
EPXS010-10238-901 IL-12p70 3090.0 0.75 <10 <10
EPXS010-12017-901 IL-17A 1030.0 0.25 <10 <10
EPXS010-10281-901 MCP-1 650 0.16 <10 <10
EPXS010-10223-901 TNF alpha 1390 0.45 <10 <10

 

Mouse high sensitivity simplex kits

Simplex Cat. No. Analyte ULOQ*
(pg/mL)
LLOQ*
(pg/mL)
Inter Assay
%CV
Intra Assay
%CV
EPXS010-20606-901 IFN gamma 1250.0 0.31 <10 <10
EPXS010-20601-901 IL-2 1820.0 0.44 <10 <10
EPXS010-20613-901 IL-4 3530.0 0.86 <10 <10
EPXS010-20603-901 IL-6 4230.0 1.03 <10 <10
EPXS010-20607-901 TNF alpha 1830.0 0.45 <10 <10

*determined for first lot

ProcartaPlex high sensitivity basic kits

To run simplex kits alone or in combination with other simplex kits, buffer reagents and plate(s) are needed. ProcartaPlex Human and Mouse High Sensitivity Basic Kits contain all necessary support reagents.

Cat. No. Description
EPXS010-10420-901 ProcartaPlex Human High Sensitivity Basic Kit
EPXS010-20440-901 ProcartaPlex Mouse High Sensitivity Basic Kit

Supporting information about ProcartaPlex assays

ProcartaPlex Supplemental Products

Find specialty diluents, buffers, streptavidin-PE conjugate to supplement ProcartaPlex assays, as well as accessory equipment such as magnets and plate washers.

ProcartaPlex Immunoassay Publication List

Browse lists of publications, organized chronologically by year, from peer reviewed journals that cite the use of ProcartaPlex immunoassays.

ProcartaPlex Analyst 1.0 Software

Learn about features of our complimentary multiplex analysis software, free to download with the purchase of ProcartaPlex kits and panels.

Custom Luminex Assays

Use our ProcartaPlex Mix & Match assay option to design your own multiplex assay for detection on Luminex systems. Learn how we can provide custom-blended and optimized panels to deliver results tailored to the design of your choice.


Multiplexing protocols