ProcartaPlex Multiplex Assays for Luminex Systems

Profile more biomarkers with less sample

Invitrogen ProcartaPlex multiplex immunoassays use the Luminex xMAP (multianalyte profiling) technology that enables the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. The technology uses differentially dyed capture beads for each target in a multiplex “ELISA-like” assay. ProcartaPlex immunoassays can profile up to 80 times more analytes using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex immunoassays provide the ability to create a unique panel with the assurance to achieve reproducible results throughout the course of your study.


Features of ProcartaPlex assays
  • Magnetic bead format for increased ease-of-use and throughput
  • More than 90% of ProcartaPlex assays can be combined with one another
  • High correlation to our ELISA assays (R2 >0.9)
  • Scalable and reproducible performance regardless of plex size
  • Largest published panel for quantitation of up to 63 analytes

ProcartaPlex kit and panel formats

ProcartaPlex immunoassays are available in multiple formats across six species (human, mouse, rat, non-human primate, porcine, and canine).

Multiplex panels are pre-configured biologically-defined and disease-defined panels using magnetic beads for quantitative multiplex analysis of multiple targets in a single sample.

View full listing of multiplex panels

Panel features

  • Choose from a variety of commonly investigated biologically- and disease-defined analyte panels.
  • Quantitatively measure secreted biomarkers in plasma, serum, other bodily fluids, and cell culture supernatants.
  • Select “convenience” panels to use the assay as configured, or select “configurable” panels to customize the assay by combining with another panel or adding compatible ProcartaPlex simplex beads.
  • Alternatively, purchase simplex kits (see next) can be purchased individually with a ProcartaPlex basic Kit (includes all non-analyte specific reagents needed to run the ProcartaPlex assay) to create your unique customized multiplex panel.
  • Selected panels are available in "Platinum" or "High-Sensitivity" formats (see sections that follow).

Simplex bead sets are magnetic bead sets used to detect individual analytes. They are designed to be added to ProcartaPlex panels to increase customization. Alternatively, multiple ProcartaPlex simplex bead sets can be combined and run using the ProcartaPlex Basic Kit that includes all non-target specific reagents needed to perform the ProcartaPlex assay.

View full listing of simplex kits

ProcartaPlex basic kits

Cat. No. Basic Kit Description
EPX010-10420-901 Human Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-40420-901 Non-Human Primate Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-20440-901 Mouse Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-50515-901 Canine Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-60460-901 Porcine Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay
EPX010-30420-901 Rat Basic Kit Includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates - needed to perform the ProcartaPlex Simplex assay

Platinum kits and panels are designed to overcome matrix effects from serum and plasma samples, thereby improving spike- and dilution-recovery results. Serum and plasma samples are complex matrixes with many components. This complexity can cause the so called matrix effect which can impact the readout of many cytokines (low spike recovery and the dilution linearity). We developed matrix type specific sample diluents to overcome these matrix and multiplex effects, achieving high performance specifications comparable to those of traditional ELISA. These newly developed surrogate matrices for dilution of serum or plasma samples are included in ProcartaPlex Platinum kits, which produce spike- and dilution-recovery results of 70–130%.

Platinum basic kits

Cat. No. Name Description
EPXP010-10421-901 Platinum Basic Kit for Plasma Samples Includes antigen standard, plasma assay diluent, detection antibody diluent, controls, wash buffers, SA-PE and plates - needed to perform the Platinum ProcartaPlex Simplex assay
EPXP010-10422-901 Platinum Basic Kit for Serum Samples Includes antigen standard, serum assay diluent, detection antibody diluent, controls, wash buffers, SA-PE and plates - needed to perform the Platinum ProcartaPlex Simplex assay

Platinum panels

Cat. No. Name Antigens
EPXP420-10200-901 Platinum ProcartaPlex Panel 1 (42plex) BDNF, Eotaxin (CCL11), GM-CSF,GRO alpha (KC/CXCL1), HGF, IFN alpha, IFN gamma, IL-10, IL-12p70, IL-13, IL-15, IL-16, IL-17A (CTLA-8), IL-1 alpha, IL-1 beta, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-9, IL-20, IL-21, IP-10, LIF, MCP-2, MIP-1 alpha (CCL3),MIP-1 beta (CCL4), Osteoprotegerin (OPG),PDGF-BB, PECAM-1, P-selectin, RANTES (CCL5), SCF, TNF alpha, TNF-RII, tPA, TSLP, VEGF-A, VEGF-D
EPXP130-10100-901 Platinum ProcartaPlex Panel 2 (13plex) IFN gamma, GM-CSF, IL-10, IL-12p70, IL-13, IL-17A (CTLA-8), IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8 (CXCL8), TNF alpha

View full listing of platinum simplex kits

ProcartaPlex high sensitivity assays are designed to measure small concentration differences in cell culture supernatants, plasma, and serum samples with 10-fold lower LLOQs (Lower Limit of Quantification) and sensitivities for all analytes in the femtogram range. Overcome the sensitivity detection limits of conventional multiplex immunoassays.

ProcartaPlex high sensitivity assays are available in two multiplex panels for human and mouse key cytokines.

ProcartaPlex Human High Sensitivity Panel (9 plex), Cat. No. EPXS090-12199-901

Simplex Cat. No. Analyte ULOQ* (pg/mL) LLOQ* (pg/mL) Inter Assay %CV Inter Assay %CV
EPXS010-10228-901 IFN gamma 260 0.31 >10 >10
EPXS010-10224-901 IL-1 beta 1330.0 0.32 >10 >10
EPXS010-10221-901 IL-2 490 0.44 >10 >10
EPXS010-10225-901 IL-4 560 0.86 >10 >10
EPXS010-10213-901 IL-6 2850 1.03 >10 >10
EPXS010-10215-901 IL-10 750.0 0.18 >10 >10
EPXS010-10238-901 IL-12p70 3090.0 0.75 >10 >10
EPXS010-12017-901 IL-17A 1030.0 0.25 >10 >10
EPXS010-10223-901 TNF alpha 1390 0.45 >10 >10



ProcartaPlex Mouse High Sensitivity Panel (5 plex), Cat. No. EPXS050-22199-901

Simplex Cat. No. Analyte ULOQ* (pg/mL) LLOQ* (pg/mL) Inter Assay %CV Inter Assay %CV
EPXS010-20606-901 IFN gamma 1250.0 0.31 >10 >10
EPXS010-20601-901 IL-2 1820.0 0.44 >10 >10
EPXS010-20613-901 IL-4 3530.0 0.86 >10 >10
EPXS010-20603-901 IL-6 4230.0 1.03 >10 >10
EPXS010-20607-901 TNF alpha 1830.0 0.45 >10 >10

*determined for first lot

ProcartaPlex high sensitivity basic kits

To run simplex kits alone or in combination with other simplex kits, buffer reagents and plate(s) are needed. ProcartaPlex Human and Mouse High Sensitivity Basic Kits contain all necessary support reagents.

Cat. No. Description
EPXS010-10420-901 ProcartaPlex Human High Sensitivity Basic Kit
EPXS010-20440-901 ProcartaPlex Mouse High Sensitivity Basic Kit

Supporting information about ProcartaPlex assays

ProcartaPlex Supplemental Products

Find specialty diluents, buffers, streptavidin-PE conjugate to supplement ProcartaPlex assays, as well as accessory equipment such as magnets and plate washers.

ProcartaPlex Immunoassay Publication List

Browse lists of publications, organized chronologically by year, from peer reviewed journals that cite the use of ProcartaPlex immunoassays.

ProcartaPlex Analyst 1.0 Software

Learn about features of our complimentary multiplex analysis software, free to download with the purchase of ProcartaPlex kits and panels.

Custom Luminex Assays

Use our ProcartaPlex Mix & Match assay option to design your own multiplex assay for detection on Luminex systems. Learn how we can provide custom-blended and optimized panels to deliver results tailored to the design of your choice.


Multiplexing protocols