Accurately quantifying total protein concentration is a key step in most experiments and workflows involving isolation, separation, and analysis of proteins. We offer a variety of assay reagents, kits, and standards for protein quantitation by fluorescent or colorimetric detection with fluorometers, spectrophotometers, and plate readers.
Additionally, our products are offered with dilution-free protein standards for added ease-of-use with reduced set up time when generating your standard curve. Dilution-Free protein standards are a set of prediluted standards, packaged in a multichannel pipette compatible format which allows you to easily transfer a full dilution series to your microplate, at once.
NEW Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standard
NEW Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards
NEW Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit
Qubit Protein Broad Range Assay
|Best-in-Class Accuracy (low protein-protein variation)||✓||✓||✓|
|Broad Dynamic Range (>2 mg/mL wide)||✓||✓|
|Fast (≤ 15 min.)||✓||✓||✓|
|Dilution-Free Standard Curve Preparation||✓||✓||✓||✓|
Dilution-Free Sample Preparation
High-Throughput (compatible with microplate format)
|Compatible with Detergents||✓||✓||✓|
Compatible with Reducing Agents
Automatic Concentration Calculations with Qubit 4
|Automatic Concentration Calculations with SkanIt Software*||✓||✓||✓|
|*Skan it software available for Multiskan FC, Multiskan SkyHigh, and Varioskan LUX microplate readers. Learn more|
We now offer Dilution-Free products, comprising both protein assays and protein standards that make protein quantification easy, fast, and accurate. Pierce Dilution-Free products allow you to minimize tedious dilutions and pipetting errors in your protein assays, delivering accurate results while reducing assay setup time by up to 80%. Learn more about our dilution-free assays.
Copper-based protein assays, including the bicinchoninic acid (BCA) and Lowry methods.
Coomassie dye-binding assays for fast and simple protein quantification.
Fluorescent Protein Assays
Fluorescence-based protein quantification detection methods provide superior sensitivity.
Pierce Protein methods – an encyclopedia of articles about protein analysis
Protein assay technical guide – tools and reagents for improved quantitation of total or specific proteins.
Protein assay compatibility table – summarizes compatible substances for several popular protein assays.
Protein Analysis Learning Center – resources for different protein analysis techniques
Protein Biology Application Notes – journal-style articles based on research applications and proof-of-concept experiments
For Research Use Only. Not for use in diagnostic procedures.