Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. The choice among available protein assays typically is based upon the compatibility of the method with the samples to be assayed. We offer assay reagents, kits and standards to determine total protein concentration of samples containing different buffer components for protein quantitation using both fluorescence-based and colorimetric assays.

Interactive protein assay selection guide

No one reagent can be considered to be the ideal or best protein assay method. Each method has its advantages and disadvantages. Use the interactive protein assay selection tool below to help select the best protein assay for your application based on the sample composition and your preferred method.

Understand your sample’s purity and concentration

The Thermo Scientific NanoDrop One UV-Vis Spectrophotometer and the Invitrogen Qubit 3 Fluorometer, when used together, provide the ability to obtain the most complete information about the concentration and quality of your DNA, RNA, or protein sample. Knowing the precise concentration and purity of your sample early helps you prevent costly troubleshooting and rework downstream.

Choose the platform that is right for you

Featured protein assay categories

High-quality, easy-to-use protein standard solutions, which can be serially diluted to create assay standard curves in protein assays to measure protein concentration. Choose Bovine Serum Albumin (BSA) Standards for measuring total protein concentration or Bovine Gamma Globulin (BGG) Standards to for measuring total protein concentration in samples containing antibody (IgG).

Specialized protein assay reagents and kits to estimate and measure specific post-translational modification (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins and phosphoproteins.

Easy-to-use colorimetric or fluorescent peptide assays that are designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

Several types of immunoassays for target-specific protein quantification, including ready-to-use ELISA kits of several varieties and ProcartaPlex assays for multiplex bead-based detection using Luminex technology and instruments.

Colorimetric and fluorescent reagents to accomplish pre-column and post-column derivatization of peptides and amino acids for analysis by HPLC detectors; includes vacuum hydrolysis tubes and quantitation standards.

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