These specialized protein assay reagents and kits allow you to estimate and measure specific post-translational modification (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins and phosphoproteins.
Glycoprotein Carbohydrate Estimation Kit

Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS). 

Assay overview

  • Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
  • Semiquantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
  • Simple procedure—completed in less than 75 minutes; All you need is this kit, a microplate, and a plate reader to determine carbohydrate content
  • Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Applications

  • Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents
  • Characterize and classify purified protein fractions from an affinity procedure
  • Assess sugar and carbohydrate contamination in non-glycosylated protein samples
Phosphoprotein Phosphate Estimation Assay Kit

Classify and identify proteins as phosphorylated (serine and threonine) and obtain semi-quantitative assessment of the phosphorylation levels.

Assay overview

  • Specific—measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr)
  • Convenient—test tube and 96-well microplate protocols included and requires less than 90 minutes to perform
  • Semi-quantitative—calculate moles of phosphate (P) per mole of protein using the included phosvitin standard
  • Customizable—format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available

Applications

  • Identify proteins as containing phosphoserine or phosphothreonine phosphorylations
  • Estimate the amount of pS and pT phosphorylation
  • Develop specific quantitative assays for well-characterized phosphoproteins of interest