23208-BSA-Standards-b-230

The optimal reference standard for protein quantification measurements is a purified sample (of known concentration) of the protein you’ll be assaying in your unknown samples. If a highly purified version of the protein of interest is not available or it is too expensive to use as the standard, the alternative is to choose a readily available, inexpensive protein that exhibits a similar response in the assay you’ll be using (i.e., similar color and magnitude).

For general protein assay work, bovine serum albumin (BSA) works well as a protein standard because highly pure, relatively inexpensive preparations of this protein are widely available. Alternatively, for measuring antibody samples or immunoglobulin-rich samples, bovine gamma globulin (BGG) is a good choice, because BGG produces a color response curve that is very similar to that of IgG.

Standards for colorimetric protein assays

  • Accurate and consistent—precisely formulated at 2.00 +/–0.03 mg/mL (compared to a NIST reference) for superior lot-to-lot consistency
  • Pure and stable—supplied in ultrapure 0.9% saline solution with 0.05% sodium azide; room temperature stable
  • Convenient—choose 1 mL ampules or a complete set containing seven ready-to-use dilutions
Product Bovine Serum Albumin (BSA) Standards Bovine Gamma Globulin (BGG) Standards
Applications
  • Use as standard for protein quantitation with samples not containing immunoglobulins in colorimetric protein assays
  • Protein recovery control for desalting and other column procedures
  • General calibration of spectrophotometer UV-lamp (absorbance at 280 nm)
  • Superior reference standard for immunoglobulin quantitation in colorimetric protein assays
  • Antibody recovery control for desalting and other column procedures
  • General calibration of spectrophotometer UV-lamp (absorbance at 280 nm)
Format 23209: 1 mL glass ampoules
23210: 50 mL polypropylene bottles
23208: complete set containing seven ready-to-use dilutions (3.5 mL each)
23212: 1 mL glass ampoules
23213: complete set containing seven ready-to-use dilutions (3.5 mL each)