Human Protein Microarrays Overview
ProtoArray services and it's associated products will be discontinued after December 31, 2018. Please contact us if there is a continued need for these products or services.
The ProtoArray Human Protein Microarray is an advanced, high-content, functional protein microarray that enables rapid profiling of thousands of biochemical interactions in as little as one day. Researchers can use the ProtoArray microarray to perform a variety of assays including biomarker identification, drug target discovery, enzyme substrate identification, antibody specificity profiling, and protein-protein interaction studies. Results can be readily measured with most commercial scanners, and the data can be analyzed using our free ProtoArray Prospector software (Figure 1).
Figure 1. (Left) The high-content human ProtoArray Human Protein Microarray contains thousands of full-length human proteins for rapid profiling and easy biomarker discovery. (Right) The ProtoArray Prospector software is a user-friendly tool that enables rapid data analysis and cross-group comparisons.
Absolute identification of your protein targets with ProtoArray Human Protein Microarray
Each ProtoArray protein array has over 9,000 human proteins printed in duplicate on a one inch by three inch slide. The proteins are produced from clones selected from the Invitrogen Ultimate ORF Collection, the first and only clone resource to offer full-insert sequenced clones in a Gateway vector. Proteins are expressed as N-terminal GST fusion proteins using a baculovirus expression system. After protein expression, proteins are purified under nondenaturing conditions. The proteins are printed at +4°C on an ultrathin layer of nitrocellulose to preserve native protein structure without the need for chemical fixation. Each slide is labeled with a unique bar code for easy tracking.
Individual microarrays on nitrocellulose-coated glass slides allow for the greatest flexibility in experimental design, with protocols for each supported application available. Some of these profiling applications (Figure 2) include:
- Immune response biomarker profiling—biomarker discovery for rapid discovery of disease markers specific to a variety of infectious diseases, cancers, and autoimmune diseases
- Enzyme substrate profiling—profiling of kinases, ubiquitin ligases, and methyltransferases for target discovery and validation
- Small molecule profiling—profiling of radiolabeled, biotinylated, or fluorescently labeled small molecules for target identification or selectivity
- Protein-protein interaction profiling—Protein-protein interactions for mapping biochemical pathways
- Antibody specificity profiling—antibody specificity profiling for research and therapeutic antibody development
Figure 2. Range of ProtoArray applications. A. Biomarker discovery, B. Profiling enzymatic activity—Kinase substrate identification,methyltransferase substrate identification, and ubiquitin ligase profiling, C. Protein-protein interactions and antibody specificity profiling, and D. Small molecule protein profiling.
Profiling with ProtoArray Protein Microarrays
ProtoArray Human Protein Microarrays enable a broad range of research applications and are available both as complete optimized kits for certain applications as well as individual arrays to support a broader range of applications. Complete kits with optimized product configurations for screening protein-protein interactions and kinases against thousands of potential native protein substrates are available. Protein-protein interaction studies can be completed in as little as one day using either a V5-tagged or biotinylated protein probe with Anti-V5-Alexa Fluor 647 Antibody or streptavidin-conjugated Alexa Fluor 647 for detection. Screening of kinases can be completed in as little as 2 days with no need for special instrumentation—detection can be done with either a phosphorimager or conventional autoradiography film.
Using the ProtoArray Human Protein Microarray is simple, and you get high-quality results in a fraction of the time. Regardless of your study, fluorescent applications can be completed in as little as one day. ProtoArray microarray applications follow the same simple workflow of incubating a sample on the array, probing with a detection reagent, and then visualizing with a fluorescent scanner or phosphorimager (Figure 3).
Figure 3. Simple, robust assay for discovering novel protein-protein interactions in as little as one day.
For Research Use Only. Not for use in diagnostic procedures.