NovaBright Beta-gal System NovaBright Dual Beta-Gal & Luciferase System NovaBright Luciferase System NovaBright SEAP System
Reporter Enzyme(s) Beta-galactosidase Luciferase, beta-galactosidase Luciferase Secreted placental alkaline phosphatase
Key Attributes Homogeneous assay does not require removal of culture medium Detection of luciferase and beta-galactosidase from the same cell extract Ideal for high-throughput screening No cell lysis required
Substrate Galacton-Star®, Gal-Star®, Galacton-Plus® Luciferin, Galacton-Plus® Luciferin CSPD®
Duration of Light Emission 60–90 min 60–90 min 60–90 min 1–2 hr
Reagent Injection Not required Required Not required Not required
Special Features Designed for assays on cells cultured in luminometer plates for high-throughput screening. Choice of reaction buffers for lysis of mammalian or yeast and mammalian cells. Two assays from one cell extract enables better precision for normalizing transfection efficiency. Linear signal from 50 fg to 100 ng, stable light emission from 10 minutes to 4-5 hours. Cells remain viable; useful for stable transfectants, time course studies, etc.
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