Protein detection and analysis—Western Blotting

Our all-encompassing range of solutions for western blotting analysis is meticulously designed and tested to meet the highest standards and minimize time and effort. Establish a robust foundation for your western blotting experiments.

Innovative Western Blotting Solutions

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Total protein normalization

Total protein normalization (TPN) for quantitative western blotting analysis offers several advantages over traditional housekeeping protein-based (HKP) normalization. One optimal protein labeling reagent for TPN is the No-Stain Protein Labeling Reagent. TPN using No-Stain Labeling Reagent exhibits good linearity of response within the concentration range typically investigated (1–80 µg of protein load). The wider dynamic range prevents the loss of valuable quantitative data due to signal saturation, a common occurrence with HKP normalization. TPN using No-Stain Labeling Reagent simplifies experimental design, helping save time and resources. Unlike HKP normalization, there is no need to verify that the expression of housekeeping proteins remains unaffected by the treatment. No-Stain Labeling Reagent is particularly well-suited for TPN due to its rapid labeling protocol, and compatibility with downstream transfer and immunoblotting. It enables sensitive and stable signal detection down to 20 ng of proteins, allowing for accurate quantitation of protein expression levels.

Learn more about TPN with No-Stain Labeling Reagent

Multiplexed fluorescent western blotting

Multiplexed western blotting is a powerful technique that offers several advantages over traditional western blotting. Its ability to simultaneously detect and quantify multiple proteins within a single sample helps increase efficiency, improves accuracy, reduces costs, and enhances data quality. It is advantageous when investigating complex biological pathways involving proteins with diverse molecular weights. The simultaneous detection of multiple protein targets in one experiment reduces experimental variability and is an excellent choice when the sample volume is limited or when analyzing large sample sets. Fluorescent western blotting requires careful planning and experiment design. Selecting the right products from the start reduces optimization and troubleshooting time and helps ensure the success of your experiment.

Design your next multiplex western blotting experiment

Western blot learning and troubleshooting

Whether you are new to western blotting, or an experienced researcher wanting to confirm your knowledge, access our educational tips and resource materials to help with every aspect of western blot analysis.

Access western blotting learning center

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Discover the new ways to do western blotting with our how-to videos.
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For Research Use Only. Not for use in diagnostic procedures.