Search Thermo Fisher Scientific
Western Blotting
Western blotting is an invaluable tool for protein biology.
Find the right set of products that can assist you with detection and analysis of your protein. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high-quality, publishable results with minimal time and effort.
Separate proteins based on molecular weight using gel electrophoresis
Transfer the protein from the gel matrix to a solid-support membrane
Detect proteins by visualizing with antibodies and substrates
Protein Electrophoresis and Western Blotting Technical Handbooks
The Protein Gel Electrophoresis and Western Blotting Handbooks are comprehensive, easy-to-use guides covering every procedural step from sample prep to imaging and data analysis.
Western blot learning and troubleshooting
Whether you are new to western blotting or an experienced researcher wanting to confirm your knowledge, access our educational tips and resource materials to help with every aspect of western blot analysis.
Innovative western blotting solutions
Boost your productivity with western blotting tools designed for efficient high-quality data generation.
Invitrogen Mini Gel System
- Forward-facing well configuration—for easy sample loading
- Less running buffer required—two separate gel chambers, only use the buffer needed for each gel
- Side-by-side tank configuration—allows for simultaneous visualization of both gels
- Compatible—fits all Invitrogen precast mini gels and SureCast handcast mini gel
Invitrogen Midi Gel System
- Load up to 26 samples per gel
- Separate chambers make it easy to run just one gel or two, conserving buffer
- Use the same chamber for room temperature wet transfers
- Multiple gel chemistries available
Invitrogen iBlot 3 Western Blot Transfer System
- Rapid transfer of proteins for up to 4 gels in as few as three minutes
- Excellent transfer efficiency
- Built-in cooling provides consistent, reproducible results
Invitrogen Power Blotter Transfer Device
- Transfer up to 4 gels in only 10 minutes
- Interchangeable blotting cassettes designed for your throughput
- Multiple transfer stack consumable choices
Bandmate Automated Western Blot Processor
- Completely automated antibody incubation and wash steps
- Use as little as 3.5 mL of antibody for mini blots or 5 mL for midi blots
- Recover primary and secondary antibodies
- Process 1–4 mini or 1–2 midi blots simultaneously
Invitrogen iBind Automated Western Devices
- Load and go—devices processes solutions using sequential lateral flow technology, with no batteries, shakers, trays, or timers required
- Antibody savings—use up to 80% less primary antibody
- Reproducibility—automated blot processing enables improved blot-to-blot consistency
- Sensitivity—iBind systems offer greater sensitivity than manual methods for many monoclonal and polyclonal antibodies
Invitrogen iBright Imaging Systems
- Powerful 9.1 megapixel (MP) camera—capture publication-quality images
- Smart Exposure acquisition technology—rapidly determine optimal exposure times
- Simple interface and a package of automated features—capture images quickly with minimal effort
- Multi-application support—image protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, & more
- Multiplex with up to 4 fluorophores
- 21 CFR Part 11 compliance support
Invitrogen Mini Gel System
- Forward-facing well configuration—for easy sample loading
- Less running buffer required—two separate gel chambers, only use the buffer needed for each gel
- Side-by-side tank configuration—allows for simultaneous visualization of both gels
- Compatible—fits all Invitrogen precast mini gels and SureCast handcast mini gel
Invitrogen Midi Gel System
- Load up to 26 samples per gel
- Separate chambers make it easy to run just one gel or two, conserving buffer
- Use the same chamber for room temperature wet transfers
- Multiple gel chemistries available
Invitrogen iBlot 3 Western Blot Transfer System
- Rapid transfer of proteins for up to 4 gels in as few as three minutes
- Excellent transfer efficiency
- Built-in cooling provides consistent, reproducible results
Invitrogen Power Blotter Transfer Device
- Transfer up to 4 gels in only 10 minutes
- Interchangeable blotting cassettes designed for your throughput
- Multiple transfer stack consumable choices
Bandmate Automated Western Blot Processor
- Completely automated antibody incubation and wash steps
- Use as little as 3.5 mL of antibody for mini blots or 5 mL for midi blots
- Recover primary and secondary antibodies
- Process 1–4 mini or 1–2 midi blots simultaneously
Invitrogen iBind Automated Western Devices
- Load and go—devices processes solutions using sequential lateral flow technology, with no batteries, shakers, trays, or timers required
- Antibody savings—use up to 80% less primary antibody
- Reproducibility—automated blot processing enables improved blot-to-blot consistency
- Sensitivity—iBind systems offer greater sensitivity than manual methods for many monoclonal and polyclonal antibodies
Invitrogen iBright Imaging Systems
- Powerful 9.1 megapixel (MP) camera—capture publication-quality images
- Smart Exposure acquisition technology—rapidly determine optimal exposure times
- Simple interface and a package of automated features—capture images quickly with minimal effort
- Multi-application support—image protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, & more
- Multiplex with up to 4 fluorophores
- 21 CFR Part 11 compliance support
Western blotting tools to help you get started
Troubleshooting Guide
Quick reference to protein electrophoresis and western blotting tips and tricks
Access Western Blot Troubleshooting booklet
Protein Research Essentials Program
Sample our products
Start here
Tool Kit
Improve your western blotting results with a collection of our technical tools
Request here
Technical note
Achieve publication quality western blot data
Download now
Don’t know where to start?
Download our product guides, explore our selection guides and education center to help you find everything you need to achieve western blotting success.
View products for gel electrophoresis and western blot analysis.
Download Western blot solutions brochure
Find the western blot bundle that works for your target protein.
BlotBuilder Interactive Product Selection Tool
Find the right protein gel for your experimental needs.
Download Protein Gel Guide
Explore the iBright Imaging Systems for easy western blot documentation.
Download iBright 1500 Imaging Systems brochure
Find the right chemiluminescent substrate.
Download Chemiluminescent HRP substrate guide
High-throughput Western blot solutions to boost your productivity.
Download brochure
Having problems with western blotting or have a question about a product?
Contact our technical support scientists for experienced troubleshooting and product advice
North America: (800) 955-6288, ext 2-3 techsupport@thermofisher.com
Europe: 00 800 5345 5345 eurotech@thermofisher.com
Outside of North America and Europe:thermofisher.com/contactus
For Research Use Only. Not for use in diagnostic procedures.