Two devices, one simple technology for automated western blot processing

Invitrogen iBind Western Systems—no shakers, no trays, no timers. Set up in minutes and walk away.


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How do iBind systems work?

iBind Western Systems are non-powered devices that automate immunodetection steps. The traditional, manual process involves preparing and replacing multiple antibody and wash solutions over several hours in a tray containing the blot of interest.

Traditional manual processing workflow

iBind traditional processing workflow

Traditional manual western processing workflow requires more than 10 hands-on steps and at least 4 hours before final detection can occur.

iBind device processing workflow

By contrast, iBind Western Systems allow all solutions to be prepared and loaded in the device at the start of the procedure, from which point all steps proceed automatically and uninterrupted by sequential lateral flow technology (SLF), i.e., simple capillary action—no electricity or batteries are required.

iBind processing

Western processing using the Invitrogen iBind and iBind Flex Western Systems require only a 15- to 30-minute setup time and no additional hands-on steps before you can move to final detection.

Video: Sequential lateral flow technology explained

Which iBind device is right for you?

Two iBind Western Systems are available: the original iBind Western Device, which accommodates one mini blot at a time, and the iBind Flex Western Device, which accommodates two mini blots, one midi blot, or up to six membrane strips at a time.

 

iBind Western Device

iBind Flex Western Device

 
Mini blot (single) Yes Yes
Mini blot (dual) No Yes
Midi blot No Yes
Vertically cut strips No Yes
  Learn more Learn more
Excellent western performance compared to manual blotting
  • The iBind System offers greater sensitivity compared to manual methods for many monoclonal and polyclonal antibodies.
  • Combine the iBind Western System with highly specific primary and secondary antibodies to achieve cleaner western blots.
  • Automated processing enables improved blot-to-blot consistency with CVs typically less than 5% (compared to manual processing that can have CVs of 13%).
 

Western blots processed on the Invitrogen iBind device show excellent sensitivity compared to western blots processed manually. (A–B) Western blots with phosphorylated Akt (left to right: 30 µg–500 ng cell extract load) were processed either on the iBind device or using standard manual western processing protocols as specified by the antibody manufacturer (monoclonal anti–phospho-Akt[Thr308] (C31E5E) primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind device detected phospho-Akt in 500 ng of cell extract, while the target was detected in 4 µg on the manually processed blot. (C–D) Western blots with cell lysate expressing CREB (left to right: 30 µg–1 µg cell lysate load) were processed either on the iBind device or using standard manual western processing protocols as specified by the antibody manufacturer (polyclonal anti-CREB primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind system detected CREB in 6 µg of cell lysate, while 10 µg of lysate was needed to detect CREB on the manually processed blot. For all blots, proteins were separated using Invitrogen Bolt Bis-Tris Plus gels on the Mini Gel Tank and transferred to PVDF membranes using the Invitrogen iBlot 2 Dry Blotting System.

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