Stripping Buffers for Western Blotting
These specially formulated buffers are designed to dissociate and strip primary and secondary antibodies from Western blots so that membranes can be reprobed under alternate conditions or with another antibody to detect a different protein target.
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent western blots to be reprobed.
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.
Which stripping buffer is right for me?
|Restore™ Stripping Buffer||Restore™ Plus Stripping||Restore™ Fluorescent western blot Stripping Buffer|
|Features||Gentle, odor-free||Robust yet gentle, odor free||Optimized for near-infrared fluorescent blotting|
|Membrane||NC and PVDF||NC and PVDF||Use with low fluorescence PVDF membrane #22860|
|Time of incubation||15-30 min at RT||5-15 mins at RT||15 min at RT|
|Select when…||Primary antibody is susceptible to stripping buffers||Using high affinity primary antibody||Using NIR labeled antibody|
|Order Now||Order Now||Order Now|
For Research Use Only. Not for use in diagnostic procedures.