28360-20X-TBS-Tween-230

Ready-to-use, pre-formulated buffers for Western blotting; includes PBS, PBS-T, TBS, TBS-T (Tween-20). Choose from dry blend packs or pouches of pre-blended powder mixtures of commonly needed buffers such as PBS and TBS for Western blot methods; simply add water to dissolve and prepare or 10X and 20X concentrated solutions of frequently used Western blot buffers; simply dilute to 1X with water and they're ready to use.

Wash buffer & detergent selection guide

  TBS PBS TBST PBST
Dry blend

28376 - 40 packs
28379 - 10 packs

28372 - 40 packs

   
Liquid conc.

28358 - 500 ml

28348 - 500 ml

28360 - 500 ml

28352 - 500 ml

Formulation
  • 25mM Tris
  • 0.15M NaCl
  • pH 7.2
  • 10mM sodium phosphate
  • 0.15M NaCl
  • pH 7.5
  • 25mM Tris
  • 0.15M NaCl
  • 0.05% Tween-20
  • pH 7.5
  • 10mM sodium phosphate
  • 0.15M NaCl
  • 0.05% Tween-20
  • pH 7.5
Application
  • ELISA, Western blotting & other immunoassays
  • Diluent or blocking buffer for plate-based assays
  • ELISA, Western blotting & other immunoassays
  • Crosslinking, biotinylation & fluorescent labeling reactions requiring an amine-free buffer
  • ELISA, Western blotting & other immunoassays
  • Diluent or blocking buffer for plate-based assays
  • ELISA, Western blotting & other immunoassays
  • Diluent or blocking buffer for plate-based assays

Like other immunoassay procedures, Western blotting consists of a series of incubations with different immunochemical reagents separated by wash steps. Washing steps are necessary to remove unbound reagents and reduce background, thereby increasing the signal-to-noise ratio.

Insufficient washing produces high background, while excessive washing may result in decreased sensitivity caused by elution of the antibody and/or antigen from the blot. As with other steps in performing a Western blot, a variety of buffers may be used. Occasionally, washing is performed in a physiological buffer such as Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) without any additives. More commonly, a detergent such as 0.05% Tween-20 Detergent (Product # 28320) is added to the buffer to help remove nonspecifically bound material.

Another common technique is to use a dilute solution of the blocking buffer along with some added detergent to help minimize background. For best results, use high-purity detergents, such as Surfact-Amps™ Detergents for Western blotting.