Thermo Scientific Pierce Fast Western Blot Kits accelerate the Western blotting process with streamlined protocols and optimized reagents that enables accuracy, sensitivity and reliability. The kits help reduce hands-on and overall blotting time to approximately one hour using any of the Thermo Scientific Pierce ECL and SuperSignal Chemiluminescent Substrates.
After transferring proteins to a membrane, traditional Western blotting procedures can take more than 4 hours to complete. Our kits provide all the reagents (except your primary antibody) necessary to complete a Western blot in about 55 minutes. The protocol is easy and requires little or no special optimization. Simply use a mouse or rabbit primary antibody at the same concentration used for typical detection with the Pierce ECL or SuperSignal Substrate. Because it is a reagent-based system, there is no investment in equipment or costly consumables to purchase. Our specially formulated blocking buffers and titered detection antibodies accelerate Western blotting by minimizing incubation times without sacrificing sensitivity.