Fulfills a wide range of application requirements

The iBright Imaging Systems offer up to five imaging modes to support your multiple application requirements. Efficiently and easily capture data from protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, and more.

Example imaging applications

Fluorescent western blots

4-plex multiplex fluorescent western blot imaged on iBright Imaging System

Chemiluminescent western blots

Multiplex chemiluminescent western blot imaged on iBright Imaging System

Combined fluorescent and chemiluminescent western blots

Multiplex chemiluminescent and fluorescent western blot imaged on iBright Imaging system

Colorimetric western blots

Colorimetric western blot imaged on iBright Imaging System

Fluorescent stained nucleic acid gels

Fluorescently stained nucleic acid gel imaged on iBright Imaging System

Fluorescent stained protein gels

Fluorescently stained protein gel imaged on iBright Imaging System

Colorimetric stained protein gels

Coomassie stained gel imaged on iBright 1500 Imaging System

Colorimetric membrane stains

Colorimetric stained western blot membrane imaged on iBright Imaging System

Colony plates

Fluorescent colony plate imaged on iBright Imaging System

Images pictured for fluorescent stained nucleic acid gels and colorimentric stained protein gels shown in pseudocolor (false color applied). Data is catured in grayscale.


Touchscreen interface

The 12.1-inch LCD touchscreen interface has a simple, logical layout of functions and features, making our systems easy to use with minimal training. Workflows are similar between imaging modes, delivering a smooth imaging experience regardless of sample type.

iBright 1500 Imaging System touchscreen interface with simple layout of functions and features.

High-resolution 9.1 megapixel camera

Camera lens

The 9.1-megapixel cooled CCD camera captures and crisp, clear, publication-quality images. Smart Exposure technology rapidly determines optimal exposure time, minimizing the potential for over- or underexposed images and the need to repeat exposures to get the desired signal


Automation streamlines operation

Mechanical rotation vs digital rotation and illustration of automatic mechanical rotation in the iBright Imager.

The iBright 1500 Series Imaging Systems automatically determine the sample position and can rotate samples left or right up to 10° on a mechanically rotating sample stage. This automation eliminates the need for repeated opening of the sample drawer to reposition your sample to achieve proper alignment.  In addition, mechanical rotation eliminates the need to digitally rotate the sample, which preserves the integrity of the data.

 

Digital rotation vs. mechanical rotation. (A) Pixels rotate with digital rotation so bands appear jagged. With mechanical rotation, the sample itself rotates, so bands remain smooth in appearance as the pixels remain aligned. (B) Graphic depicting iBright Imaging System sample stage before and after rotation.

In addition, iBright Imaging Systems automatically adjust the focus for each level of zoom, to maximally utilize the 22.5 cm x 18.0 cm field of view. If imaging a single blot, the camera will automatically zoom up to 2X zoom (1-2X zoom is mechanical zoom with iBright 1500 Series Imaging Systems, 1-2X zoom is digital zoom with the iBright CL750 Imaging system).  Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage and thus reduces focal length. The iBright 1500 Series Imaging Systems also provide additional 1-4X digital zoom for a combined zoom level of 1-8X.

Illustration of the 8x zoom function in iBright Imaging Systems using a multiplex fluorescent western blot.

Zoom function. (A) Unzoomed image of a fluorescent western blot. (B) Blot at 2X zoom. (C) Blot at 4X zoom. (D) Blot at 8X zoom. (blot not repositioned during successive zooms)


Large field of view in a small footprint

Illustration of iBright Imaging system field of view using 4 mini multiplex fluorescent western blots.

The large field of view (22.5 x 18.0 cm) enables capture of up to 4 mini blots or gels.


Accelerate your work with fluorescent multiplexed western blots

Expand the possibilities—The iBright FL1500 model features five fluorescence channels, permitting up to 4-color fluorescent western blot multiplexing and expanding your possibilities for studying multiple proteins in a single blot. Obtain meaningful and representative comparisons to enhance your experiments. Smart Exposure technology further improves acquisition of multiplex fluorescent western blot data by ensuring that signal-to-noise ratios are optimized for each fluorescent channel separately.

 

 

Illustration of the fluorescent western blot imaging features in iBright FL1500

Green-LED transilluminator

iBright Imaging System green-LED transilluminator for nucleic acid gel imaging

The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offers several additional benefits.

 

No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.

 

No mercury waste: UV transilluminator bulbs may contain mercury, a hazardous substance, and therefore require special care for handling and disposal.

 

Longer lifetime: LED bulbs have a substantially longer real-time life than fluorescent UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.


Protein normalization workflow

To account for variability in samples due to unequal protein sample concentration, inconsistent sample loading onto the gel, and uneven transfer of protein from the gel to membrane, an extra step called normalization is often performed by comparing proteins of interest to internal housekeeping protein controls such as GAPDH, β-tubulin, β-actin, and more. Although housekeeping proteins have been the historically popular choice for normalization, newer strategies involving the comparison of the protein of interest to total lane protein have emerged as a potentially more accurate alternative.

 

To support the methods best suited for your experiment, iBright Imaging Systems and iBright Analysis Software provide multiple quantitation and normalization options to monitor or mathematically compensate for experimental or sample variability.

 

Quantitative western blot analysis using Invitrogen No-Stain Protein Labeling Reagent demonstrating linearity of protein normalization

Total protein normalization using the No-Stain Protein Labeling Reagent. Invitrogen™ Bolt™ 4-12% Bis-Tris Plus gels were loaded with HeLa lysate ranging from 10 to 50 µg and electrophoresed using MES running buffer. Proteins from the gels were transferred onto mini PVDF membranes using the Invitrogen™ iBlot™ 2 Gel Transfer Device with iBlot 2 Transfer Stacks (P0 protocol for 7 minutes). The PVDF membranes were washed twice for 2 min with 20 mL of ultrapure water on a rotating platform, whereupon they were labeled with 10 mL of a working solution of No-Stain Protein Labeling Reagent on a rotating platform for 10 minutes. The membranes were then washed 3 times for 2 minutes with 20 mL of ultrapure water on a rotating platform, followed by addition of Invitrogen™ primary antibodies against β-actin (Cat. No. AM4302), GAPDH (Cat. No. 398600), and α-tubulin (Cat. No. 138000), and Invitrogen™ Goat Anti–Mouse IgG Alexa Fluor™ Plus 680 secondary antibody (Cat. No. A21058). The blot was imaged using the Invitrogen™ iBright™ FL1500 Imaging System. The iBright software was used to quantitate the total protein signal in the lanes. The R² value of the plotted data for the entire range of total protein signal was determined to be 0.9990, whereas the R² values for β-actin, GAPDH, and α-tubulin were 0.8851, 0.9438, and 0.8332, respectively.