Simplify western blot and gel documentation
The Invitrogen iBright Imaging Systems balance powerful features and hardware in a package that is easy to use for researchers of all experience levels. Feel at home with our touchscreen interface carefully crafted to provide smooth image capture experience. Get publication quality data fast with a powerful 9.1MP camera and a suite of automated features. Streamline image analysis with built-in on-instrument software or our standalone iBright Analysis Software. Understand your data better with straightforward total lane protein and house-keeping protein normalization workflows. Read on to find the right iBright Imager for your needs.
iBright CL750 Imaging System |
iBright CL1500 Imaging System |
iBright FL1500 Imaging System |
|
| Core essential western blot and gel imaging-transition from darkroom and film-based detection with ease | Expanded application support with many of the same high-performance specifications as the iBright FL1500 imager | Maximum application support including up to 4-channel multiplex western blot imaging | |
| Main supported imaging applications | |||
|---|---|---|---|
| Colorimetric stained protein gels | |||
| Fluorescent stained protein gels | |||
| Fluorescent stained nucleic acid gels | |||
| Colorimetric stained membranes | Limited* | ||
| Chemiluminescent western blots | |||
| Colorimetric western blots | |||
| Fluorescent western blots | |||
| Qualitative visible imaging applications** | |||
| Translucent objects (e.g., colony plates) | |||
| Opaque objects (e.g., 2D strips, TLC plates, leaf sections) | |||
| GFP expression in multiwell (e.g., 6-well) plates | |||
| Camera | |||
| Detector | Cooled 16-bit CCD, 65,535 shades of gray | ||
| Resolution | 9.1 megapixels | ||
| Lens | Fixed, 25 mm, f/0.95 | ||
| Field of view | 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels) | ||
| Binning modes | 1x1, 2x2, 4x4 (high-resolution/sensitivity settings) | 1x1, 2x2, 3x3, 4x4, 5x5, 6x6, 8x8 (maximum flexibility for adjusting resolution and sensitivity based on needs) | |
| Zoom | 1–2x, digital (digital zoom reduces the effective resolution of the zoomed image) | 1–8x (1–2x mechanical + 1–4x digital) (Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage, reducing focal length) | |
| System interface | |||
| Touchscreen | 12.1 inch capacitive LCD display, 1,024 x 768 pixels | ||
| Storage and connectivity | |||
| USB | 2 USB (2.0) | ||
| Network | Ethernet port, Connect (cloud-based) connectivity, optional Wi-Fi (adapter sold separately) | ||
| Image file format | G2i (proprietary), TIFF, JPG, PNG | ||
| Hard drive | 64 GB SSD | 256 GB SSD | |
| System Hardware | |||
| Sampler drawer | Manual operation | Automatic open/close with automatic sample rotation (up to 10°) | |
| Filter set | 2 filters (0 excitation, 2 emission) | 4 filters (2 excitation, 2 emission) | 12 filter (6 excitation, 6 emission) |
| Illumination source |
|
|
|
| System software | |||
| Automated featured |
|
||
| Stand-alone analysis applications | iBright Analysis Software: desktop version (macOS™ or Windows™ operating systems) and Connect (cloud-based) version | ||
| Shipping, warranty, and upgrades | |||
| Dimensions | (L x W x H): 68 x 38 x 60 cm | ||
| Weight | Approximately 47 kg (105 lb) | Approximately 50 kg (110 lbs) | |
| Warranty | 1 year from date of purchase | 2 years from date of purchase | |
| Upgradability | No | Yes, to FL1500 imager | No |
* Membranes must be imaged when wet, using Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes or Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes.
** Applications provide a qualitative visualization of the object or confirmation of the presence of signal. Not recommended for quantitation.
The iBright 1500 Series Imaging Systems consist of two models: the iBright FL1500 Imaging System and the iBright CL1500 Imaging System. The iBright FL1500 Imaging System is our premier instrument, and supports the main imaging applications of fluorescent, chemiluminescent, and colorimetric western blots, in addition to fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. We also offer the iBright CL1500 Imaging System, which features the same imaging capabilities as the iBright FL1500 Imaging System, but without fluorescent western blot imaging functionality.
Features of the iBright 1500 Series Imaging Systems:
- 9.1 MP cooled CCD camera: high sensitivity and dynamic range to help enable the detection of subtle differences in samples
- Five fluorescence channels: multiplex and capture up to four proteins in a single blot for more meaningful and representative experiments (FL1500 model only)
- Smart Exposure technology: provides rapid determination of optimal exposure time
- Simple interface: clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
- Advanced automated features: automatic sample rotation, automatic zoom, and automatic focus help streamline image capture
- 22.5 x 18.0-cm field of view: large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
- Green LED-based transilluminator: effectively excite popular DNA dyes such as ethidium bromide and Invitrogen SYBR Green dyes with an alternative to UV-based transilluminators
- Flexible connectivity: export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to Connect (cloud-based platform)
- Multiple image analysis options: perform densitometry, quantitation, and normalization directly on iBright using the on-instrument software, or with our standalone iBright Analysis Software (available in both desktop and cloud-based versions)
We are working on creating new videos for the iBright 1500 Series Imaging Systems.
For reference below are videos for our previous iBright 1000 Series Imaging Systems. We’ve made many improvements with our 1500 Series models – so check back soon for our new videos!
Watch our short video to learn more about the main features and functions
Capture images and analyze data from your western blots and gels efficiently and easily using the Invitrogen iBright Imaging Systems. With an artful blend of modern design and performance, enhance your imaging workflow today.
How to capture images with Invitrogen iBright Imaging Systems
Capturing high-quality images of western blots and gels is easy with Invitrogen iBright Imaging Systems. Watch our short how-to video to visualize the straightforward image capture workflow.
How to analyze images with Invitrogen iBright Imaging Systems
Analyze image data directly on Invitrogen iBright Imaging Systems. Watch our short how-to video to see the smooth on-board data analysis workflow.
How to export images with Invitrogen iBright Imaging Systems
Exporting images captured on Invitrogen iBright Imaging Systems is simple. Watch our short how-to video to view the uncomplicated image export workflow.
Brochure
Manuals
Application notes
| iBright CL750 Imaging System |
iBright CL1500 Imaging System |
iBright FL1500 Imaging System |
|
| Core essential western blot and gel imaging-transition from darkroom and film-based detection with ease | Expanded application support with many of the same high-performance specifications as the iBright FL1500 imager | Maximum application support including up to 4-channel multiplex western blot imaging | |
| Main supported imaging applications | |||
|---|---|---|---|
| Colorimetric stained protein gels | |||
| Fluorescent stained protein gels | |||
| Fluorescent stained nucleic acid gels | |||
| Colorimetric stained membranes | Limited* | ||
| Chemiluminescent western blots | |||
| Colorimetric western blots | |||
| Fluorescent western blots | |||
| Qualitative visible imaging applications** | |||
| Translucent objects (e.g., colony plates) | |||
| Opaque objects (e.g., 2D strips, TLC plates, leaf sections) | |||
| GFP expression in multiwell (e.g., 6-well) plates | |||
| Camera | |||
| Detector | Cooled 16-bit CCD, 65,535 shades of gray | ||
| Resolution | 9.1 megapixels | ||
| Lens | Fixed, 25 mm, f/0.95 | ||
| Field of view | 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels) | ||
| Binning modes | 1x1, 2x2, 4x4 (high-resolution/sensitivity settings) | 1x1, 2x2, 3x3, 4x4, 5x5, 6x6, 8x8 (maximum flexibility for adjusting resolution and sensitivity based on needs) | |
| Zoom | 1–2x, digital (digital zoom reduces the effective resolution of the zoomed image) | 1–8x (1–2x mechanical + 1–4x digital) (Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage, reducing focal length) | |
| System interface | |||
| Touchscreen | 12.1 inch capacitive LCD display, 1,024 x 768 pixels | ||
| Storage and connectivity | |||
| USB | 2 USB (2.0) | ||
| Network | Ethernet port, Connect (cloud-based) connectivity, optional Wi-Fi (adapter sold separately) | ||
| Image file format | G2i (proprietary), TIFF, JPG, PNG | ||
| Hard drive | 64 GB SSD | 256 GB SSD | |
| System Hardware | |||
| Sampler drawer | Manual operation | Automatic open/close with automatic sample rotation (up to 10°) | |
| Filter set | 2 filters (0 excitation, 2 emission) | 4 filters (2 excitation, 2 emission) | 12 filter (6 excitation, 6 emission) |
| Illumination source |
|
|
|
| System software | |||
| Automated featured |
|
||
| Stand-alone analysis applications | iBright Analysis Software: desktop version (macOS™ or Windows™ operating systems) and Connect (cloud-based) version | ||
| Shipping, warranty, and upgrades | |||
| Dimensions | (L x W x H): 68 x 38 x 60 cm | ||
| Weight | Approximately 47 kg (105 lb) | Approximately 50 kg (110 lbs) | |
| Warranty | 1 year from date of purchase | 2 years from date of purchase | |
| Upgradability | No | Yes, to FL1500 imager | No |
* Membranes must be imaged when wet, using Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes or Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes.
** Applications provide a qualitative visualization of the object or confirmation of the presence of signal. Not recommended for quantitation.
The iBright 1500 Series Imaging Systems consist of two models: the iBright FL1500 Imaging System and the iBright CL1500 Imaging System. The iBright FL1500 Imaging System is our premier instrument, and supports the main imaging applications of fluorescent, chemiluminescent, and colorimetric western blots, in addition to fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. We also offer the iBright CL1500 Imaging System, which features the same imaging capabilities as the iBright FL1500 Imaging System, but without fluorescent western blot imaging functionality.
Features of the iBright 1500 Series Imaging Systems:
- 9.1 MP cooled CCD camera: high sensitivity and dynamic range to help enable the detection of subtle differences in samples
- Five fluorescence channels: multiplex and capture up to four proteins in a single blot for more meaningful and representative experiments (FL1500 model only)
- Smart Exposure technology: provides rapid determination of optimal exposure time
- Simple interface: clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
- Advanced automated features: automatic sample rotation, automatic zoom, and automatic focus help streamline image capture
- 22.5 x 18.0-cm field of view: large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
- Green LED-based transilluminator: effectively excite popular DNA dyes such as ethidium bromide and Invitrogen SYBR Green dyes with an alternative to UV-based transilluminators
- Flexible connectivity: export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to Connect (cloud-based platform)
- Multiple image analysis options: perform densitometry, quantitation, and normalization directly on iBright using the on-instrument software, or with our standalone iBright Analysis Software (available in both desktop and cloud-based versions)
We are working on creating new videos for the iBright 1500 Series Imaging Systems.
For reference below are videos for our previous iBright 1000 Series Imaging Systems. We’ve made many improvements with our 1500 Series models – so check back soon for our new videos!
Watch our short video to learn more about the main features and functions
Capture images and analyze data from your western blots and gels efficiently and easily using the Invitrogen iBright Imaging Systems. With an artful blend of modern design and performance, enhance your imaging workflow today.
How to capture images with Invitrogen iBright Imaging Systems
Capturing high-quality images of western blots and gels is easy with Invitrogen iBright Imaging Systems. Watch our short how-to video to visualize the straightforward image capture workflow.
How to analyze images with Invitrogen iBright Imaging Systems
Analyze image data directly on Invitrogen iBright Imaging Systems. Watch our short how-to video to see the smooth on-board data analysis workflow.
How to export images with Invitrogen iBright Imaging Systems
Exporting images captured on Invitrogen iBright Imaging Systems is simple. Watch our short how-to video to view the uncomplicated image export workflow.
Brochure
Manuals
Application notes
For Research Use Only. Not for use in diagnostic procedures.
