iBright Imaging Systems

Simplify western blot and gel documentation

The Invitrogen iBright Imaging Systems balance powerful features and hardware in a package that is easy to use for researchers of all experience levels. Feel at home with our touchscreen interface carefully crafted to provide smooth image capture experience. Get publication quality data fast with a powerful 9.1MP camera and a suite of automated features. Streamline image analysis with built-in on-instrument software or our standalone iBright Analysis Software. Understand your data better with straightforward total lane protein and house-keeping protein normalization workflows. Read on to find the right iBright Imager for your needs.

  iBright CL750 Imaging System
iBright CL750 Imaging System
iBright CL1500 Imaging System
iBright CL1000 Imaging System
iBright FL1500 Imaging System
iBright FL1000 Imaging System
  Core essential western blot and gel imaging-transition from darkroom and film-based detection with ease Expanded application support with many of the same high-performance specifications as the iBright FL1500 imager Maximum application support including up to 4-channel multiplex western blot imaging
Main supported imaging applications
Colorimetric stained protein gels      
Fluorescent stained protein gels      
Fluorescent stained nucleic acid gels      
Colorimetric stained membranes Limited*    
Chemiluminescent western blots      
Colorimetric western blots      
Fluorescent western blots      
Qualitative visible imaging applications**
Translucent objects (e.g., colony plates)      
Opaque objects (e.g., 2D strips, TLC plates, leaf sections)      
GFP expression in multiwell (e.g., 6-well) plates      
Camera
Detector Cooled 16-bit CCD, 65,535 shades of gray
Resolution 9.1 megapixels
Lens Fixed, 25 mm, f/0.95
Field of view 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels)
Binning modes 1x1, 2x2, 4x4 (high-resolution/sensitivity settings) 1x1, 2x2, 3x3, 4x4, 5x5, 6x6, 8x8 (maximum flexibility for adjusting resolution and sensitivity based on needs)
Zoom 1–2x, digital (digital zoom reduces the effective resolution of the zoomed image) 1–8x (1–2x mechanical + 1–4x digital) (Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage, reducing focal length)
System interface
Touchscreen 12.1 inch capacitive LCD display, 1,024 x 768 pixels
Storage and connectivity
USB 2 USB (2.0)
Network Ethernet port, Connect (cloud-based) connectivity, optional Wi-Fi (adapter sold separately)
Image file format G2i (proprietary), TIFF, JPG, PNG
Hard drive 64 GB SSD 256 GB SSD
System Hardware
Sampler drawer Manual operation Automatic open/close with automatic sample rotation (up to 10°)
Filter set 2 filters (0 excitation, 2 emission) 4 filters (2 excitation, 2 emission) 12 filter (6 excitation, 6 emission)
Illumination source
  • Green LED (470–550 nm) transilluminator
  • Green LED (470–550 nm)  transilluminator
  • Epi white LED
  • Green LED (470–550 nm) transilluminator 
  • Epi white LED
  • Epi near-IR LED
System software
Automated featured
  • Automatic zoom
  • Automatic focus
  • Automatic exposure (Smart Exposure)
  • Automatic on-board image analysis
Stand-alone analysis applications iBright Analysis Software: desktop version (macOS™ or Windows™ operating systems) and Connect (cloud-based) version
Shipping, warranty, and upgrades
Dimensions (L x W x H): 68 x 38 x 60 cm
Weight Approximately 47 kg (105 lb) Approximately 50 kg (110 lbs)
Warranty 1 year from date of purchase 2 years from date of purchase
Upgradability No Yes, to FL1500 imager No

* Membranes must be imaged when wet, using Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes or Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes.
** Applications provide a qualitative visualization of the object or confirmation of the presence of signal. Not recommended for quantitation.

The iBright 1500 Series Imaging Systems consist of two models: the iBright FL1500 Imaging System and the iBright CL1500 Imaging System. The iBright FL1500 Imaging System is our premier instrument, and supports the main imaging applications of fluorescent, chemiluminescent, and colorimetric western blots, in addition to fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. We also offer the iBright CL1500 Imaging System, which features the same imaging capabilities as the iBright FL1500 Imaging System, but without fluorescent western blot imaging functionality.

Features of the iBright 1500 Series Imaging Systems:

  • 9.1 MP cooled CCD camera: high sensitivity and dynamic range to help enable the detection of subtle differences in samples
  • Five fluorescence channels: multiplex and capture up to four proteins in a single blot for more meaningful and representative experiments (FL1500 model only)
  • Smart Exposure technology: provides rapid determination of optimal exposure time
  • Simple interface: clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
  • Advanced automated features: automatic sample rotation, automatic zoom, and automatic focus help streamline image capture
  • 22.5 x 18.0-cm field of view: large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
  • Green LED-based transilluminator: effectively excite popular DNA dyes such as ethidium bromide and Invitrogen SYBR Green dyes with an alternative to UV-based transilluminators
  • Flexible connectivity: export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to Connect (cloud-based platform)
  • Multiple image analysis options: perform densitometry, quantitation, and normalization directly on iBright using the on-instrument software, or with our standalone iBright Analysis Software (available in both desktop and cloud-based versions)

iBright Imaging Systems

Capture images and analyze data from your western blots and gels efficiently and easily using the Invitrogen iBright Imaging Systems. With a blend of modern design and performance, enhance your imaging workflow today.

Capture high quality images fast with Invitrogen iBright Imaging Systems

Learn about the Invitrogen iBright Imaging Systems' Smart Exposure technology, and see how simple it is to capture an image.

Customize exposure routines easily with Invitrogen iBright Imaging Systems

Learn about the Invitrogen iBright Imaging Systems' signal accumulation, multi-exposure, and region-of-interest Smart Exposure features.

Analyze images quickly with Invitrogen iBright Imaging Systems

Analyze images directly on Invitrogen iBright Imaging Systems with a smooth on-board analysis workflow.

How to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System for fast, easy, and accurate quantitative western blotting

This video demonstrates how fast and easy it is to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System to capture and quantitate western blotting data using total protein normalization.

Scientist: Guillaume Pidoux

Scientist: Guillaume Pidoux
Location: France
Abstract: My group focuses on the spatiotemporal regulation of cAMP signaling in cardiac physiological and pathophysiological conditions.

The heart ensures that peripheral organs are perfused with blood and nutrients. Any alterations of this function lead to heart injury and failure. A-kinase anchoring proteins (AKAPs) are a family of scaffolding proteins that anchors protein kinase-A (PKA), and thus plays a central role in the regulation of cardiac function under physiological and pathological conditions.

We focus on understanding the direct role of AKAPs in cardiac physiology and during the occurrence of cardiomyopathies. We utilize molecular biology tools to direct shRNA mediated knockdown and reconstitution assays combined with biochemical assays (i.e., immunoblot, co-immunoprecipitation, TR-FRET and Alpha-Screen). We further support our studies with state-of-the-art confocal imaging (i.e., live-cell FRAP & FLIP, proximity ligation assay and super-resolution imaging).

Furthermore, we aim to develop protein-protein interactor (PPI) disrupting peptides to counteract anchored-PKA dependent cellular processes that are modified during cardiac pathologies. These specific PPIs could serve as new potential specific cardiac therapeutic agents.

Favorite feature of the iBright system: Easy-to-use interface allowing the acquisition of excellent resolution pictures.
  iBright CL750 Imaging System
iBright CL750 Imaging System
iBright CL1500 Imaging System
iBright CL1000 Imaging System
iBright FL1500 Imaging System
iBright FL1000 Imaging System
  Core essential western blot and gel imaging-transition from darkroom and film-based detection with ease Expanded application support with many of the same high-performance specifications as the iBright FL1500 imager Maximum application support including up to 4-channel multiplex western blot imaging
Main supported imaging applications
Colorimetric stained protein gels      
Fluorescent stained protein gels      
Fluorescent stained nucleic acid gels      
Colorimetric stained membranes Limited*    
Chemiluminescent western blots      
Colorimetric western blots      
Fluorescent western blots      
Qualitative visible imaging applications**
Translucent objects (e.g., colony plates)      
Opaque objects (e.g., 2D strips, TLC plates, leaf sections)      
GFP expression in multiwell (e.g., 6-well) plates      
Camera
Detector Cooled 16-bit CCD, 65,535 shades of gray
Resolution 9.1 megapixels
Lens Fixed, 25 mm, f/0.95
Field of view 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels)
Binning modes 1x1, 2x2, 4x4 (high-resolution/sensitivity settings) 1x1, 2x2, 3x3, 4x4, 5x5, 6x6, 8x8 (maximum flexibility for adjusting resolution and sensitivity based on needs)
Zoom 1–2x, digital (digital zoom reduces the effective resolution of the zoomed image) 1–8x (1–2x mechanical + 1–4x digital) (Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage, reducing focal length)
System interface
Touchscreen 12.1 inch capacitive LCD display, 1,024 x 768 pixels
Storage and connectivity
USB 2 USB (2.0)
Network Ethernet port, Connect (cloud-based) connectivity, optional Wi-Fi (adapter sold separately)
Image file format G2i (proprietary), TIFF, JPG, PNG
Hard drive 64 GB SSD 256 GB SSD
System Hardware
Sampler drawer Manual operation Automatic open/close with automatic sample rotation (up to 10°)
Filter set 2 filters (0 excitation, 2 emission) 4 filters (2 excitation, 2 emission) 12 filter (6 excitation, 6 emission)
Illumination source
  • Green LED (470–550 nm) transilluminator
  • Green LED (470–550 nm)  transilluminator
  • Epi white LED
  • Green LED (470–550 nm) transilluminator 
  • Epi white LED
  • Epi near-IR LED
System software
Automated featured
  • Automatic zoom
  • Automatic focus
  • Automatic exposure (Smart Exposure)
  • Automatic on-board image analysis
Stand-alone analysis applications iBright Analysis Software: desktop version (macOS™ or Windows™ operating systems) and Connect (cloud-based) version
Shipping, warranty, and upgrades
Dimensions (L x W x H): 68 x 38 x 60 cm
Weight Approximately 47 kg (105 lb) Approximately 50 kg (110 lbs)
Warranty 1 year from date of purchase 2 years from date of purchase
Upgradability No Yes, to FL1500 imager No

* Membranes must be imaged when wet, using Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes or Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes.
** Applications provide a qualitative visualization of the object or confirmation of the presence of signal. Not recommended for quantitation.

The iBright 1500 Series Imaging Systems consist of two models: the iBright FL1500 Imaging System and the iBright CL1500 Imaging System. The iBright FL1500 Imaging System is our premier instrument, and supports the main imaging applications of fluorescent, chemiluminescent, and colorimetric western blots, in addition to fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. We also offer the iBright CL1500 Imaging System, which features the same imaging capabilities as the iBright FL1500 Imaging System, but without fluorescent western blot imaging functionality.

Features of the iBright 1500 Series Imaging Systems:

  • 9.1 MP cooled CCD camera: high sensitivity and dynamic range to help enable the detection of subtle differences in samples
  • Five fluorescence channels: multiplex and capture up to four proteins in a single blot for more meaningful and representative experiments (FL1500 model only)
  • Smart Exposure technology: provides rapid determination of optimal exposure time
  • Simple interface: clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
  • Advanced automated features: automatic sample rotation, automatic zoom, and automatic focus help streamline image capture
  • 22.5 x 18.0-cm field of view: large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
  • Green LED-based transilluminator: effectively excite popular DNA dyes such as ethidium bromide and Invitrogen SYBR Green dyes with an alternative to UV-based transilluminators
  • Flexible connectivity: export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to Connect (cloud-based platform)
  • Multiple image analysis options: perform densitometry, quantitation, and normalization directly on iBright using the on-instrument software, or with our standalone iBright Analysis Software (available in both desktop and cloud-based versions)

iBright Imaging Systems

Capture images and analyze data from your western blots and gels efficiently and easily using the Invitrogen iBright Imaging Systems. With a blend of modern design and performance, enhance your imaging workflow today.

Capture high quality images fast with Invitrogen iBright Imaging Systems

Learn about the Invitrogen iBright Imaging Systems' Smart Exposure technology, and see how simple it is to capture an image.

Customize exposure routines easily with Invitrogen iBright Imaging Systems

Learn about the Invitrogen iBright Imaging Systems' signal accumulation, multi-exposure, and region-of-interest Smart Exposure features.

Analyze images quickly with Invitrogen iBright Imaging Systems

Analyze images directly on Invitrogen iBright Imaging Systems with a smooth on-board analysis workflow.

How to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System for fast, easy, and accurate quantitative western blotting

This video demonstrates how fast and easy it is to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System to capture and quantitate western blotting data using total protein normalization.

Scientist: Guillaume Pidoux

Scientist: Guillaume Pidoux
Location: France
Abstract: My group focuses on the spatiotemporal regulation of cAMP signaling in cardiac physiological and pathophysiological conditions.

The heart ensures that peripheral organs are perfused with blood and nutrients. Any alterations of this function lead to heart injury and failure. A-kinase anchoring proteins (AKAPs) are a family of scaffolding proteins that anchors protein kinase-A (PKA), and thus plays a central role in the regulation of cardiac function under physiological and pathological conditions.

We focus on understanding the direct role of AKAPs in cardiac physiology and during the occurrence of cardiomyopathies. We utilize molecular biology tools to direct shRNA mediated knockdown and reconstitution assays combined with biochemical assays (i.e., immunoblot, co-immunoprecipitation, TR-FRET and Alpha-Screen). We further support our studies with state-of-the-art confocal imaging (i.e., live-cell FRAP & FLIP, proximity ligation assay and super-resolution imaging).

Furthermore, we aim to develop protein-protein interactor (PPI) disrupting peptides to counteract anchored-PKA dependent cellular processes that are modified during cardiac pathologies. These specific PPIs could serve as new potential specific cardiac therapeutic agents.

Favorite feature of the iBright system: Easy-to-use interface allowing the acquisition of excellent resolution pictures.