Let your colors show with iBright Imaging Systems

iBright 1500 Series Imaging Systems offer up to five imaging modes to support your applications. Efficiently and easily capture data from protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, and more. Get the most from your investment by having the freedom to choose from multiple detection strategies to meet your experimental needs.

Image mode What kind of signal can be captured?
Protein gel Colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)
Nucleic acid gel Ethidium bromide and Invitrogen SYBR stains
Chemiluminescent blot Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Fluorescent blot Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates)
Universal Custom mode to image samples containing multiple signals, such as chemiluminescence, fluorescence, colorimetric stains, and/or visible images; image display is similar to fluorescent blot mode and allows one to assign false color to any sample

With the five fluorescent channels of the iBright FL1500 Imaging System, one can image with many different fluorophore options. Up to 4-channel multiplexing is possible, utilizing fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. The table below shows available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.

Fluorescence channels on the iBright FL1500 Imaging System

Excitation channel Filter range (nm) Emission channel Filter range (nm)
EX1 455–485 EM1 515–564
EX2 515–545 EM2 568–617
EX3 608–632 EM3 675–720
EX4 610–660 EM4 710–730
EX5 745–765 EM5 800–850

Examples of fluorophores that can be detected using the iBright FL1500 Imaging System

Alexa Fluor Dyes Excitation channel Emission channel
Alexa Fluor 488, Alexa Fluor Plus 488 455-485 nm (EX1) 515-564 nm (EM1)
Alexa Fluor 546 515-545 nm (EX2) 568-617 nm (EM2)
Alexa Fluor 555, Alexa Fluor Plus 555 515-545 nm (EX2) 568-617 nm (EM2)
Alexa Fluor 568 515-545 nm (EX2) 568-617 nm (EM2)
Alexa Fluor 594 608-632 nm (EX3) 675-720 nm (EM3)
Alexa Fluor 633 608-632 nm (EX3) 675-720 nm (EM3)
Alexa Fluor 647, Alexa Fluor Plus 647 608-632 nm (EX3) 675-720 nm (EM3)
Alexa Fluor 660 610-660 nm (EX4) 675-720 nm (EM3)
Alexa Fluor 680, Alexa Fluor Plus 680 610-660 nm (EX4) 710-730 nm (EM4)
Alexa Fluor 750 745-765 nm (EX5) 800-850 nm (EM5)
Alexa Fluor 790 745-765 nm (EX5) 800-850 nm (EM5)
Alexa Fluor Plus 800 745-765 nm (EX5) 800-850 nm (EM5)
DyLight Dyes Excitation channel Emission channel
DyLight 488 455-485 nm (EX1) 515-564 nm (EM1)
DyLight 549/550 515-545 nm (EX2) 568-617 nm (EM2)
DyLight 594 608-632 nm (EX3) 675-720 nm (EM3)
DyLight 633 608-632 nm (EX3) 675-720 nm (EM3)
DyLight 649/650 608-632 nm (EX3) 675-720 nm (EM3)
DyLight 680 610-660 nm (EX4) 710-730 nm (EM4)
DyLight 755 745-765 nm (EX5) 800-850 nm (EM5)
DyLight 800 745-765 nm (EX5) 800-850 nm (EM5)
Western Dots, Qdots Excitation channel Emission channel
Qdots 525 455-485 nm (EX1) 515-564 nm (EM1)
Qdots 565 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 585 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 605 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 625 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 655 455-485 nm (EX1) 675-720 nm (EM3)
Qdots 705 455-485 nm (EX1) 675-720 nm (EM3)
Qdots 800 455-485 nm (EX1) 810-850 nm (EM5)
WesternDots 585 455-485 nm (EX1) 568-617 nm (EM2)
WesternDots 625 455-485 nm (EX1) 568-617 nm (EM2)
WesternDots 655 455-485 nm (EX1) 675-720 nm (EM3)
WesternDots 800 455-485 nm (EX1) 800-850 nm (EM5)
Traditional Dyes and Cyanin Excitation channel Emission channel
Fluorescein/FITC 455-485 nm (EX1) 515-564 nm (EM1)
Green Fluorescent Protein (GFP) 455-485 nm (EX1) 515-564 nm (EM1)
Oregon Green 455-485 nm (EX1) 515-564 nm (EM1)
Red Fluorescent Protein (RFP) 515-545 nm (EX2) 568-617 nm (EM2)
Rhodamine 123 455-485 nm (EX1) 515-564 nm (EM1)
Tetramethyl Rhodamine (TRITC) 515-545 nm (EX2) 568-617 nm (EM2)
Texas Red 608-632 nm (EX3) 675-720 nm (EM3)
Cyanin 2 (Cy2) 455-485 nm (EX1) 515-564 nm (EM1)
Cyanin 3 (Cy3) 515-545 nm (EX2) 568-617 nm (EM2)
Cyanin 5 (Cy5) 608-632 nm (EX3) 675-720 nm (EM3)
Cyanin 5.5 (Cy5.5) 610-660 nm (EX4) 710-750 nm (EM4)
Cyanin 7 (Cy7) 745-765 nm (EX5) 800-850 nm (EM5)
IRDye, StarBright Excitation channel Emission channel
IRDye 650 608-632 nm (EX3) 675-720 nm (EM3)
IRDye 680 610-660 nm (EX4) 710-730 nm (EM4)
IRDye 700 610-660 nm (EX4) 710-730 nm (EM4)
IRDye 800 745-765 nm (EX5) 800-850 nm (EM5)
StarBright Blue 520 455-485 nm (EX1) 515-564 nm (EM1)
StarBright Blue 700 455-485 nm (EX1) 710-730 nm (EM4)

Examples of chemiluminescent reagents that can be detected using the iBright FL1500 and CL1500 Imaging Systems

  FL1500 CL1500
SuperSignal West Pico Plus    
SuperSignal West Dura    
SuperSignal West Femto    
Pierce ECL Plus    
Pierce ECL     
Other AP and HRP chemiluminescent substrates    

Example protein gel stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

  FL1500 CL1500
Coomassie stains    
Silver stains    
SYPRO Ruby    
SYPRO Orange    
SYPRO Tangerine    
SYPRO Red    
Coomassie Fluor Orange    
Deep purple stains    
Oriole fluorescent stain    
Flamingo fluorescent stain    

Examples of nucleic acid dyes and stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

  FL1500 CL1500
Ethidium bromide    
Hoechst blue    
SYBR Green I    
SYBR Green II    
SYBR Safe    
SYBR Gold    

With the five fluorescent channels of the iBright FL1500 Imaging System, one can image with many different fluorophore options. Up to 4-channel multiplexing is possible, utilizing fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. The table below shows available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.

Fluorescence channels on the iBright FL1500 Imaging System

Excitation channel Filter range (nm) Emission channel Filter range (nm)
EX1 455–485 EM1 515–564
EX2 515–545 EM2 568–617
EX3 608–632 EM3 675–720
EX4 610–660 EM4 710–730
EX5 745–765 EM5 800–850

Examples of fluorophores that can be detected using the iBright FL1500 Imaging System

Alexa Fluor Dyes Excitation channel Emission channel
Alexa Fluor 488, Alexa Fluor Plus 488 455-485 nm (EX1) 515-564 nm (EM1)
Alexa Fluor 546 515-545 nm (EX2) 568-617 nm (EM2)
Alexa Fluor 555, Alexa Fluor Plus 555 515-545 nm (EX2) 568-617 nm (EM2)
Alexa Fluor 568 515-545 nm (EX2) 568-617 nm (EM2)
Alexa Fluor 594 608-632 nm (EX3) 675-720 nm (EM3)
Alexa Fluor 633 608-632 nm (EX3) 675-720 nm (EM3)
Alexa Fluor 647, Alexa Fluor Plus 647 608-632 nm (EX3) 675-720 nm (EM3)
Alexa Fluor 660 610-660 nm (EX4) 675-720 nm (EM3)
Alexa Fluor 680, Alexa Fluor Plus 680 610-660 nm (EX4) 710-730 nm (EM4)
Alexa Fluor 750 745-765 nm (EX5) 800-850 nm (EM5)
Alexa Fluor 790 745-765 nm (EX5) 800-850 nm (EM5)
Alexa Fluor Plus 800 745-765 nm (EX5) 800-850 nm (EM5)
DyLight Dyes Excitation channel Emission channel
DyLight 488 455-485 nm (EX1) 515-564 nm (EM1)
DyLight 549/550 515-545 nm (EX2) 568-617 nm (EM2)
DyLight 594 608-632 nm (EX3) 675-720 nm (EM3)
DyLight 633 608-632 nm (EX3) 675-720 nm (EM3)
DyLight 649/650 608-632 nm (EX3) 675-720 nm (EM3)
DyLight 680 610-660 nm (EX4) 710-730 nm (EM4)
DyLight 755 745-765 nm (EX5) 800-850 nm (EM5)
DyLight 800 745-765 nm (EX5) 800-850 nm (EM5)
Western Dots, Qdots Excitation channel Emission channel
Qdots 525 455-485 nm (EX1) 515-564 nm (EM1)
Qdots 565 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 585 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 605 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 625 455-485 nm (EX1) 568-617 nm (EM2)
Qdots 655 455-485 nm (EX1) 675-720 nm (EM3)
Qdots 705 455-485 nm (EX1) 675-720 nm (EM3)
Qdots 800 455-485 nm (EX1) 810-850 nm (EM5)
WesternDots 585 455-485 nm (EX1) 568-617 nm (EM2)
WesternDots 625 455-485 nm (EX1) 568-617 nm (EM2)
WesternDots 655 455-485 nm (EX1) 675-720 nm (EM3)
WesternDots 800 455-485 nm (EX1) 800-850 nm (EM5)
Traditional Dyes and Cyanin Excitation channel Emission channel
Fluorescein/FITC 455-485 nm (EX1) 515-564 nm (EM1)
Green Fluorescent Protein (GFP) 455-485 nm (EX1) 515-564 nm (EM1)
Oregon Green 455-485 nm (EX1) 515-564 nm (EM1)
Red Fluorescent Protein (RFP) 515-545 nm (EX2) 568-617 nm (EM2)
Rhodamine 123 455-485 nm (EX1) 515-564 nm (EM1)
Tetramethyl Rhodamine (TRITC) 515-545 nm (EX2) 568-617 nm (EM2)
Texas Red 608-632 nm (EX3) 675-720 nm (EM3)
Cyanin 2 (Cy2) 455-485 nm (EX1) 515-564 nm (EM1)
Cyanin 3 (Cy3) 515-545 nm (EX2) 568-617 nm (EM2)
Cyanin 5 (Cy5) 608-632 nm (EX3) 675-720 nm (EM3)
Cyanin 5.5 (Cy5.5) 610-660 nm (EX4) 710-750 nm (EM4)
Cyanin 7 (Cy7) 745-765 nm (EX5) 800-850 nm (EM5)
IRDye, StarBright Excitation channel Emission channel
IRDye 650 608-632 nm (EX3) 675-720 nm (EM3)
IRDye 680 610-660 nm (EX4) 710-730 nm (EM4)
IRDye 700 610-660 nm (EX4) 710-730 nm (EM4)
IRDye 800 745-765 nm (EX5) 800-850 nm (EM5)
StarBright Blue 520 455-485 nm (EX1) 515-564 nm (EM1)
StarBright Blue 700 455-485 nm (EX1) 710-730 nm (EM4)

Examples of chemiluminescent reagents that can be detected using the iBright FL1500 and CL1500 Imaging Systems

  FL1500 CL1500
SuperSignal West Pico Plus    
SuperSignal West Dura    
SuperSignal West Femto    
Pierce ECL Plus    
Pierce ECL     
Other AP and HRP chemiluminescent substrates    

Example protein gel stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

  FL1500 CL1500
Coomassie stains    
Silver stains    
SYPRO Ruby    
SYPRO Orange    
SYPRO Tangerine    
SYPRO Red    
Coomassie Fluor Orange    
Deep purple stains    
Oriole fluorescent stain    
Flamingo fluorescent stain    

Examples of nucleic acid dyes and stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

  FL1500 CL1500
Ethidium bromide    
Hoechst blue    
SYBR Green I    
SYBR Green II    
SYBR Safe    
SYBR Gold    
Visistat reference component