The iBright Imaging Systems were designed to make western blot gel imaging easy. Click on each subtopic below to learn more about how our iBright Imaging systems will help you streamline your western blot and gel imaging experience.
|iBright CL1000 Imaging System
||iBright FL1000 Imaging System
|Chemiluminescence western blot||Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)|
|Fluorescent western blots||Not available||Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor, Alexa Fluor Plus, Thermo Scientific DyLight dyes)|
|Stained protein gels and blots||Colorimetric staining of gels (e.g. Coomassie dye, silver) and membranes (e.g., Ponceau S, Thermo Scientific MemCode stain)|
|Stained nucleic acid gels||Ethidium bromide and Invitrogen SYBR dye staining|
Load and go—Insert samples using the motorized drawer. The touchscreen interface has a simple, logical layout of functions and features, making iBright Imaging Systems easy to use with minimal training. Workflows are similar between imaging modes—for a smooth imaging experience regardless of sample type.
9.1 MP camera
High sensitivity—Equipped with a powerful 9.1 megapixel (MP) cooled CCD camera, iBright instruments provide robust imaging potential, to enable the detection of subtle differences in protein expression.
iBright Imaging Systems feature a powerful 9.1 MP camera for greater sensitivity compared to instruments with a lower-resolution camera.
Smart exposure technology
Capture crystal-clear images—Smart exposure technology rapidly determines optimal exposure time, which helps minimize the potential for over- or underexposed images, and the need to repeat exposures to get the desired signal.
Smart exposure provides an optimal image without the need to capture multiple images. Smart exposure and manual exposure on the iBright Imaging System were used to analyze HeLa lysates serially diluted and probed for HDAC1. The same blot was imaged using Smart Exposure technology or four manual exposure times. The set of images on the right are the same images on the left but with the saturation feature of the iBright FL1000 Imager turned on, which highlights saturated pixels red.
Hassle-free sample alignment, focus, and zoom—rather than having to open the sample door and repeatedly reposition your sample to achieve proper alignment, iBright Imaging Systems automatically determine the sample position and can rotate samples left or right up to 10° on a mechanically rotating sample stage. Mechanical rotation is preferable to digital rotation, as digital rotation can lead to data alterations.
The iBright Imaging Systems automatically determine if the sample requires zoom in order to maximally utilize the field of view. If imaging a single blot, the camera will move toward the sample (up to 2x zoom). This puts the camera physically closer to the sample, which further maximizes sensitivity, as the light travels a shorter distance to the camera sensor. The iBright Imaging Systems automatically adjust focus for each level of zoom, producing crystal-clear images.
Zoom function. Blot captured at 1x zoom (left panel). The same blot captured at 2x zoom (right panel).
Field of view
Big field of view, small footprint—The iBright Imaging Systems feature a light path design that helps enable a large functional viewing area in a relatively small instrument footprint (68 x 38 x 60cm).
Accelerate your research—The iBright FL1000 model features five fluorescence channels, permitting up to 4-color fluorescent western blot multiplexing and expanding your possibilities for studying multiple proteins in a single blot. Obtain meaningful and representative comparisons to enhance your experiments. Smart exposure technology further improves acquisition of multiplex fluorescent western blot data, because signal-to-noise ratios are optimized for each fluorescent channel separately.
Go-Green--The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer many additional benefits.
Ethidium bromide stained DNA gel
SYBR Safe stained DNA gel
No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
No mercury waste: UV transilluminator bulbs contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
Longer lifetime: LED bulbs have a substantially longer real-time life than UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.
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Structural biology/Protein interactions
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For Research Use Only. Not for use in diagnostic procedures.