The iBright 1500 Series Imaging Systems were designed to make western blot and gel imaging easy. Click on each subtopic below to learn more.

Applications

The core applications you need and the specialty applications you want

The iBright 1500 Series Imaging Systems offer up to five imaging modes to support your multiple applications. Efficiently and easily capture data from protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, and more.

Image mode What kind of signal can be captured?
Protein gel Colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)
Nucleic acid gel Ethidium bromide and Invitrogen SYBR stains
Chemiluminescent blot Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Fluorescent blot Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates)
Universal Custom mode to image samples containing multiple signals, such as chemiluminescence, fluorescence, colorimetric stains, and/or visible images; image display is similar to fluorescent blot mode and allows one to assign false color to any sample

Example imaging applications

Fluorescent western blots

Fluorescent western blots

Chemiluminescent western blots

Chemiluminescent western blots

Combined fluorescent and chemiluminescent western blots

Combined fluorescent and chemiluminescent western blots

Colorimetric western blots

Colorimetric western blots

Fluorescent stained nucleic acid gels

Fluorescent stained nucleic acid gels

Fluorescent stained protein gels

Fluorescent stained protein gels

Colorimetric stained protein gels

Colorimetric stained protein gels

Colorimetric membrane stains

Colorimetric membrane stains

Colony plates

Colony plates

Images pictured for fluorescent stained nucleic acid gels and colorimetric stained protein gels shown in pseudocolor (false color applied). Data is captured in grayscale.


Interface

Load and go—Our 12.1 inch LCD touchscreen interface has a simple, logical layout of functions and features, making our systems easy to use with minimal training. Workflows are similar between imaging modes—for a smooth imaging experience regardless of sample type.


9.1 MP camera

Get publication-quality data fast

ibright-camera-image

Capture crisp, clear, publication-quality images with a 9.1 megapixel cooled CCD camera.

Smart Exposure technology rapidly determines optimal exposure time, minimizing the potential for over- or underexposed images and the need to repeat exposures to get the desired signal.


Automated features

Rather than having to open the sample drawer and repeatedly reposition your sample to achieve proper alignment, iBright 1500 Series Imaging Systems automatically determine the sample position and can rotate samples left or right up to 10° on a mechanically rotating sample stage. Mechanical rotation eliminates the need to digitally rotate the sample, which preserves the integrity of the data, as digital rotation can lead to data alterations.

Zoom function

Digital rotation vs. mechanical rotation. (A) Pixels rotate with digital rotation so bands appear jagged. With mechanical rotation, the sample itself rotates, so bands remain smooth in appearance as the pixels remain aligned. (B) Graphic depicting iBright Imaging System sample stage before and after rotation.

In addition, iBright 1500 Series Imaging Systems automatically determine if the sample requires zoom in order to maximally utilize the 22.5 cm x 18.0 cm field of view. If imaging a single blot, the camera will mechanically move toward the sample up to 2X zoom (8X with additional digital zoom). Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage and thus reducing focal length. iBright 1500 Series Imaging Systems automatically adjust focus for each level of zoom, producing A crystal-clear images.

Zoom function

Zoom function. (A) Unzoomed image of a fluorescent western blot. (B) Blot at 2X zoom. (C) Blot at 4X zoom. (D) Blot at 8X zoom. (blot not repositioned during successive zooms)


Field of view

Big field of view, small footprint—The iBright Imaging Systems feature a light path design that helps enable a large functional viewing area in a relatively small instrument footprint (68 x 38 x 60cm).

Field of view
The large field of view (22.5 x 18.0 cm) enables capture of up to 4 mini blots or gels.

Filter sets

Accelerate your research—The iBright FL1500 model features five fluorescence channels, permitting up to 4-color fluorescent western blot multiplexing and expanding your possibilities for studying multiple proteins in a single blot. Obtain meaningful and representative comparisons to enhance your experiments. Smart Exposure technology further improves acquisition of multiplex fluorescent western blot data, because signal-to-noise ratios are optimized for each fluorescent channel separately.

Filter sets pre-installed in iBright FL1500 Imaging Systems for visible light range (RGB) and near infrared range (NIR) fluorescent western blotting applications.

Excitation channel Filter range (nm) Emission channel Filter range (nm) Example compatible fluorophores
EX1 455-485 EM1 515-564 Alexa Fluor Plus 488, Alexa Fluor 488
EX2 515-545 EM2 568-617 Alexa Fluor Plus 555, Alexa Fluor 546
EX3 608-632 EM3 675-720 Alexa Fluor Plus 647, Alexa Fluor 594
EX4 610-660 EM4 710-730 Alexa Fluor Plus 680, Alexa Fluor 680
EX5 745-765 EM5 800-850 Alexa Fluor Plus 800, Alexa Fluor 790

Green-LED transilluminator

Green-LED transilluminator

Go-Green:The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer many additional benefits.

No harmful UV rays

No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.

No mercury waste

No mercury waste: UV transilluminator bulbs contain mercury, a hazardous substance, and therefore require special care for handling and disposal.

Longer lifetime

Longer lifetime: LED bulbs have a substantially longer real-time life than UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.


Citations

Cancer studies

Below are a list of citations referencing the iBright Imaging Systems platform, and will be updated periodically.

General

Hirpara J, et al. (2018) Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance. Redox Biol Dec 17 ePub:101076.

Brain

Li J, et al. (2018) Downregulation of Survivin gene expression affects Ionizing radiation resistance of human T98 glioma cells. Cell Mol Neurobiol 38:861–868.

Colorectal

Taş I, et al. (2018) Physciosporin suppresses the proliferation, motility and tumourigenesis of colorectal cancer cells. Phytomedicine 56:10−20.

Endometrial

Li J, et al. (2018) MicroRNA 423 promotes proliferation, migration and invasion and induces chemoresistance of endometrial cancer cells. Exp Ther Med 16(5):4213−4224.

Squamous cell carcinoma

Lin SR, Wen CF (2018) PG-priming enhances doxorubicin influx to trigger necrotic and autophagic cell death in oral squamous cell carcinoma. J Clin Med 7(10):e375.

Disease study: Biomarker research

Zhang G, et al. (2018) Detection of MiR-29a in plasma patients with lumbarspinal stenosis and the clinical significance. Mol Med Rep 18(1):223−229.

Karaszi K, et al. (2019) Increased placental expression of placental protein 5 (PP5) / tissue factor pathway inhibitor-2 (TFPI-2) in women with preeclampsia and HELLP syndrome: Relevance to impaired trophoblast invasion? Placenta 76:30−39.

Drug mechanism

Bellot GL, et al. (2019) MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management. Redox Biol 20:307−320.

Method development

Orlomoski R, et al. (2019) Rapid and efficient purification of Drosophila homeodomain transcription factors for biophysical characterization. Protein Expr Purif 158:9−14.

Microorganisms and model organisms

Zhao K, et al. (2019) TesG Is a type I secretion effector of Pseudomonas aeruginosa that suppresses the host immune response during chronic infection. Nat Microbiol 4(3):459−469.

Park C, et al. (2019) Species-specific inhibition of antiviral protein kinase R by capripoxviruses and vaccinia virus. Ann NY Acad Sci 1438(1):18−29.

Neurobiology

Ha JS, et al. (2018) Anti-amyloidogenic properties of an ethyl acetate fraction from Actinidia arguta in Aβ1–42-induced ICR mice. Food Funct 9(6):3264−3277.

Kim JM, et al. (2018) Ethyl acetate fraction from persimmon (Diospyros kaki) ameliorates cerebral neuronal loss and cognitive deficit via the JNK/Akt pathway in TMT-induced mice. Int J Mol Sci 19:E1499.

Park, SB, et al. (2018) Aruncus dioicus var. kamtschaticus extract suppresses mitochondrial apoptosis induced-neurodegeneration in trimethyltin injected ICR mice. J Food Biochem 42(6):e12667.

Schultz ML, et al. (2018) Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD. Nat Commun 9(1):3671.

Plant sciences

Mishra R, et al. (2018) Can-miRn37a mediated suppression of ethylene response factors enhances the resistance of chilli against anthracnose pathogen Colletotrichum truncatum L. Plant Sci 267:135–147.

Structural biology/Protein interactions

Thawani A, et al. XMAP215 Is a microtubule nucleation factor that functions synergistically with the y-tubulin ring complex. Nat Cell Biol 20(5):575−585,

Other

Ran L, et al. (2018) Humoral factors contributing to ectopic bone formation induced by medical biomaterials. J Biomater Tissue Eng 8:880–886.

Huang TL, et al. (2019) The therapeutic effect of aucubin-supplemented hyaluronic acid on interleukin-1 beta-stimulated human articular chondrocytes. Phytomedicine 53:1−8.

Qian Y, et al. (2018) Lactobacillus plantarum CQPC11 isolated from sichuan pickled cabbages antagonizes d-galactose-induced oxidation and aging in mice. Molecules 23(11):e3026.

Depledge DP, et al. (2019) Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen. Nat Commun 10(1):754.

Rageul J, et al. (2019) Conditional degradation of SDE2 by the Arg/N-End rule pathway regulates stress response at replication forks. Nucleic Acids Res Jan 30 ePub:gkz054.