The iBright Imaging Systems were designed to make western blot gel imaging easy. Click on each subtopic below to learn more about how our iBright Imaging systems will help you streamline your western blot and gel imaging experience.

Applications

  iBright CL1000 Imaging System
iBright CL1000 Imaging System
iBright FL1000 Imaging System
iBright FL1000 Imaging System
Applications
Chemiluminescence western blot Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Fluorescent western blots Not available Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor, Alexa Fluor Plus, Thermo Scientific DyLight dyes)
Stained protein gels and blots Colorimetric staining of gels (e.g. Coomassie dye, silver) and membranes (e.g., Ponceau S, Thermo Scientific MemCode stain)
Stained nucleic acid gels Ethidium bromide and Invitrogen SYBR dye staining

See the list of dyes and reagents that are compatible with iBright imagers


Interface

Load and go—Insert samples using the motorized drawer. The touchscreen interface has a simple, logical layout of functions and features, making iBright Imaging Systems easy to use with minimal training. Workflows are similar between imaging modes—for a smooth imaging experience regardless of sample type.

iBright-user-interface

9.1 MP camera

High sensitivity—Equipped with a powerful 9.1 megapixel (MP) cooled CCD camera, iBright instruments provide robust imaging potential, to enable the detection of subtle differences in protein expression.

9.1 MP camera

iBright Imaging Systems feature a powerful 9.1 MP camera for greater sensitivity compared to instruments with a lower-resolution camera.

ibright-camera-2
Comparison of detection sensitivity and dynamic range for film and the iBright FL1000 Imaging System. Analysis reveals the better signal linearity and dynamic range of signals acquired using the iBright FL1000 Imaging System compared to film (right panel).

Smart exposure technology

Capture crystal-clear images—Smart exposure technology rapidly determines optimal exposure time, which helps minimize the potential for over- or underexposed images, and the need to repeat exposures to get the desired signal.

Smart exposure provides an optimal image without the need to capture multiple images

Smart exposure provides an optimal image without the need to capture multiple images. Smart exposure and manual exposure on the iBright Imaging System were used to analyze HeLa lysates serially diluted and probed for HDAC1. The same blot was imaged using Smart Exposure technology or four manual exposure times. The set of images on the right are the same images on the left but with the saturation feature of the iBright FL1000 Imager turned on, which highlights saturated pixels red.


Automated features

Hassle-free sample alignment, focus, and zoom—rather than having to open the sample door and repeatedly reposition your sample to achieve proper alignment, iBright Imaging Systems automatically determine the sample position and can rotate samples left or right up to 10° on a mechanically rotating sample stage. Mechanical rotation is preferable to digital rotation, as digital rotation can lead to data alterations.

Digital vs mechanical sample rotation
Digital vs mechanical sample rotation. Left: digital rotation (10°)—pixels rotate with digital rotation, so bands appear jagged. Right: Mechanical rotation (10°)—sample rotates so bands remain smooth as pixels remain aligned
Digital vs mechanical sample rotation
Sample rotation. Graphic depicting iBright Imaging System stage before and after rotation.

The iBright Imaging Systems automatically determine if the sample requires zoom in order to maximally utilize the field of view. If imaging a single blot, the camera will move toward the sample (up to 2x zoom). This puts the camera physically closer to the sample, which further maximizes sensitivity, as the light travels a shorter distance to the camera sensor. The iBright Imaging Systems automatically adjust focus for each level of zoom, producing crystal-clear images.

Zoom function

Zoom function. Blot captured at 1x zoom (left panel). The same blot captured at 2x zoom (right panel).


Field of view

Big field of view, small footprint—The iBright Imaging Systems feature a light path design that helps enable a large functional viewing area in a relatively small instrument footprint (68 x 38 x 60cm).

Field of view
The large field of view (22.5 x 18.0 cm) enables capture of up to 4 mini blots or gels.

Filter sets

Accelerate your research—The iBright FL1000 model features five fluorescence channels, permitting up to 4-color fluorescent western blot multiplexing and expanding your possibilities for studying multiple proteins in a single blot. Obtain meaningful and representative comparisons to enhance your experiments. Smart exposure technology further improves acquisition of multiplex fluorescent western blot data, because signal-to-noise ratios are optimized for each fluorescent channel separately.

Filter sets
Filter sets pre-installed in iBright FL1000 imaging systems for visible light (RGB) and near infrared (NIR) fluorescent western blotting applications.

Green-LED transilluminator

Go-Green--The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer many additional benefits.

Ethidium bromide stained DNA gel

Ethidium bromide stained DNA gel

SYBR Safe stained DNA gel

SYBR Safe stained DNA gel

No harmful UV rays

No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.

No mercury waste

No mercury waste: UV transilluminator bulbs contain mercury, a hazardous substance, and therefore require special care for handling and disposal.

Longer lifetime

Longer lifetime: LED bulbs have a substantially longer real-time life than UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.


Citations

Disease study: Biomarker research

Zhang G, et al. (2018) Detection of MiR-29a in plasma patients with lumbarspinal stenosis and the clinical significance. Mol Med Rep 18(1):223−229.

Karaszi K, et al. (2019) Increased placental expression of placental protein 5 (PP5) / tissue factor pathway inhibitor-2 (TFPI-2) in women with preeclampsia and HELLP syndrome: Relevance to impaired trophoblast invasion? Placenta 76:30−39.

Drug mechanism

Bellot GL, et al. (2019) MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management. Redox Biol 20:307−320.

Method development

Orlomoski R, et al. (2019) Rapid and efficient purification of Drosophila homeodomain transcription factors for biophysical characterization. Protein Expr Purif 158:9−14.

Microorganisms and model organisms

Zhao K, et al. (2019) TesG Is a type I secretion effector of Pseudomonas aeruginosa that suppresses the host immune response during chronic infection. Nat Microbiol 4(3):459−469.

Park C, et al. (2019) Species-specific inhibition of antiviral protein kinase R by capripoxviruses and vaccinia virus. Ann NY Acad Sci 1438(1):18−29.

Neurobiology

Ha JS, et al. (2018) Anti-amyloidogenic properties of an ethyl acetate fraction from Actinidia arguta in Aβ1–42-induced ICR mice. Food Funct 9(6):3264−3277.

Kim JM, et al. (2018) Ethyl acetate fraction from persimmon (Diospyros kaki) ameliorates cerebral neuronal loss and cognitive deficit via the JNK/Akt pathway in TMT-induced mice. Int J Mol Sci 19:E1499.

Park, SB, et al. (2018) Aruncus dioicus var. kamtschaticus extract suppresses mitochondrial apoptosis induced-neurodegeneration in trimethyltin injected ICR mice. J Food Biochem 42(6):e12667.

Schultz ML, et al. (2018) Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD. Nat Commun 9(1):3671.

Plant sciences

Mishra R, et al. (2018) Can-miRn37a mediated suppression of ethylene response factors enhances the resistance of chilli against anthracnose pathogen Colletotrichum truncatum L. Plant Sci 267:135–147.

Structural biology/Protein interactions

Thawani A, et al. XMAP215 Is a microtubule nucleation factor that functions synergistically with the y-tubulin ring complex. Nat Cell Biol 20(5):575−585,

Other

Ran L, et al. (2018) Humoral factors contributing to ectopic bone formation induced by medical biomaterials. J Biomater Tissue Eng 8:880–886.

Huang TL, et al. (2019) The therapeutic effect of aucubin-supplemented hyaluronic acid on interleukin-1 beta-stimulated human articular chondrocytes. Phytomedicine 53:1−8.

Qian Y, et al. (2018) Lactobacillus plantarum CQPC11 isolated from sichuan pickled cabbages antagonizes d-galactose-induced oxidation and aging in mice. Molecules 23(11):e3026.

Depledge DP, et al. (2019) Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen. Nat Commun 10(1):754.

Rageul J, et al. (2019) Conditional degradation of SDE2 by the Arg/N-End rule pathway regulates stress response at replication forks. Nucleic Acids Res Jan 30 ePub:gkz054.