iBlot® 2 Dry Blotting SystemThe revolutionary iBlot® Dry Blotting System just got better.

Introducing the new and improved iBlot® 2 Dry Blotting System. Enjoy fast western transfer without sacrificing efficiency and uniformity. The new iBlot® 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. Now, with a new touch screen, less consumable waste, and sturdy design, fast western transfer never looked better.

Transfer proteins quickly and efficiently from gel to membrane in 7 minutes or less

  • High detection sensitivity and evenness
  • Increased blotting reliability and reproducibility
  • Flexible gel-size formats and membrane types
  • Simple, user-friendly system
  • Ability to create new custom programs
  • Built-in tutorial and application notes
  • High-quality and more compact transfer stacks

Comparison of western transfer systems

iBlot® 2 Gel Transfer DeviceComparison of the iBlot® 2 Dry Blotting System with conventional semi-dry and wet transfer systems.

iBlot® 2 Dry Blotting System technical resources

iBlot® 2 Gel Transfer DeviceRegister your iBlot® 2 Dry Blotting System, find FAQs, and download the manual and quick reference card. 

Resources for the original iBlot® Gel Transfer Device

Original iBlot® Gel Transfer DeviceFind application notes, FAQs, literature citations and more for the original iBlot® Gel Transfer Device.

Save when you order the Bolt® Welcome Pack with iBlot® 2 Dry Blotting System

Bolt™ Welcome Pack with iBlot® 2 Dry Blotting System  

This special package offers everything you need for the entire workflow, from protein separation to western blotting. With the Bolt® Welcome Pack with iBlot® 2 Dry Blotting System, you’ll receive:

  • iBlot® 2 Gel Transfer Device
  • iBlot® 2 Nitrocellulose Regular Stacks (10 stacks)
  • Mini Gel Tank
  • Bolt® 4–12% Bis-Tris Plus Gel, 10 Well (10 gels)
  • Bolt® buffers and reagent

Order the Welcome Pack

Faster, more efficient protein transfer

Self-contained unit for faster, more convenient transfers

The iBlot® 2 Dry Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. A self-contained unit, the iBlot® device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes, offering the convenience of a bufferless, plug-and-play system.

High transfer efficiency

In addition to ease and convenience, the iBlot® system offers high-efficiency protein transfers for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, you’ll achieve more accurate detection using less sample.

Fits your gel size

The iBlot® Dry Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size, fitting Bolt®) Bis-Tris Plus gels, Novex® Midi Gels (8 x 13 cm), E-PAGE™ 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm).


Firmware Update

Note for IE users: (1) right-click the above “Download” link; (2) click “Save Target As”; (3) Change file name to “image.ib2”; (4) Select file type as “All Files”; (5) click “Save”

iBlot2 firmware upgrade instructions