Our ultimate western blot transfer system
Enjoy fast western blot transfer without sacrificing efficiency and uniformity. The iBlot 2 Dry Blotting System utilizes innovative pre-assembled dry transfer stacks, with transfer buffer incorporated into gel matrices, so there’s no need to prepare messy transfer buffers. Just insert your gel and go!
Self-contained unit for faster, more convenient transfers
The iBlot 2 Dry Blotting System was designed to deliver a high degree of convenience. The iBlot 2 Dry Blotting System is a true self-contained, plug-and-play system, with an integrated power supply, pre-packaged ready-to-use transfer stacks. Setup, teardown, and cleanup only takes minutes compared to the much more cumbersome and hands-on steps required for wet-tank transfer. The iBlot 2 Dry Blotting System has a simple touchscreen interface and pre-programmed optimized transfer programs, with the option to customize programs to suit your needs.
High transfer efficiency
In addition to ease of use and convenience, the iBlot 2 Dry Blotting System offers high-efficiency protein transfers for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, it helps you achieve more accurate detection using less sample. Transfer proteins in 7 minutes and move on quickly to the rest of your western blot processing.
Fits your gel size and works with your gel chemistry
The iBlot 2 Dry Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size (8 x 13 cm), E-PAGE 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm). The system is also compatible with all protein gel chemistries (tris-gylcine, bis-tris, tris-acetate, and tricine).
| Specifications | |
|---|---|
| Capacity | 1-2 mini, or 1 midi, or 1 E-PAGE gel |
| Compatibility | 1.0-1.5 mm thick gels |
| Transfer time | 6-10 min |
| Transfer buffer | No buffer required |
| Programs | Pre-programmed with six methods for transfer of proteins from various gel types or create custom programs |
| Power supply | Internal (no additional power supply needed) |
| Dimensions | 37 x 20 x 11 cm |
| Weight | 2.3 kg |
| Electrical Parameters | 100-240 V, 50/60 Hz, 6.3 A |
The iBlot 2 Dry Blotting System is based on the dry blotting concept, utilizing a unique gel matrix technology. The iBlot 2 Transfer Stack consists of a Bottom Stack and a Top Stack sandwiching a pre-run gel and a nitrocellulose (0.2 µm) or PVDF (0.2 µm) membrane. The iBlot 2 Transfer Stack is assembled with the blotting membrane on the anode side, and a pre-run gel on the cathode side.
Schematic of iBlot 2 Transfer Stack showing the flow of current |
 |
Complete transfer of proteins from the gel to the blotting membrane is accomplished in approximately 7–8 minutes. The rapid transfer without the need for external power supply or premade buffers is possible due to the following features of the iBlot 2 Dry Blotting System:
- The gel matrix of the Bottom and Top Stack incorporate the appropriate anode and cathode buffers to act as ion reservoirs. This format eliminates the need for premade buffers or soaked filter paper, and minimizes handling that can lead to inconsistent performance.
- The copper anode does not generate oxygen gas as a result of water electrolysis, resulting in increased transfer consistency. Conventional inert electrodes present in other blotting systems result in oxygen generation, which can result in blotting distortion.
- The design of the iBlot 2 Gel Transfer Device reduces the distance between the electrodes and the integrated power supply. This unique design combined with the gel matrix technology of iBlot 2 Transfer Stacks allows the system to generate high field strength and increase the transfer speed.
How to use the iBlot 2 Dry Blotting System
Learn how to transfer your proteins from polyacrymide gels to nitrocellulose or PDVF membranes in a little as 7 minutes with this detailed step-by-step video.
Get high efficiency transfers in a fraction of the time
A successful western blot experiment relies heavily on the transfer efficiency of proteins from your gel to the blotting membrane. Traditional wet transfer offers high efficiency, but at the cost of significant hands-on processing time and transfer time. iBlot 2 Dry Blotting System offers the same high quality transfer but with substantial improvements in speed and convenience.
Chemiluminescent detection
High transfer efficiencies achieved using the iBlot Dry Blotting System. A431 lystate was separated on Novex Tris-glycine gels and transferred to nitrocellulose using a traditional wet tank system or the iBlot 2 Dry Blotting System. The membrane was probed for EGFR, Hsp90, PDI and p23 and detected using SuperSignal West Dura Extended duration chemiluminescent substrate.
Multiplex fluorescent detection
High transfer efficiencies achieved using the iBlot Dry Blotting System. A431 lystate was separated on NuPAGE Bis-Tris gels and transferred to nitrocellulose using a traditional wet tank system or the iBlot 2 Dry Blotting System. The membrane was probed for EGFR, Hsp90, tubulin and p23.
Efficient transfers across various protein gel chemistries
The iBlot 2 Gel transfer devices works with various protein gel chemistries providing uniformity and efficiency in protein transfer.
 |
Get a jumpstart and save with our starter kits!
Our starter kits include everything you need to start transferring your proteins with the Invitrogen iBlot 2 Gel Transfer Device.
Option 1: nitrocellulose membranes
Includes:
- iBlot 2 Gel Transfer Device
- iBlot 2 Transfer Stacks, nitrocellulose, regular size (2 boxes, 20 stacks)
Option 2: PVDF membranes
Includes:
- iBlot 2 Gel Transfer Device
- iBlot 2 Transfer Stacks, PVDF, regular size (2 boxes, 20 stacks)
Still using your original iBlot device? Upgrade to an iBlot2 Dry Blotting System Today.
Manuals
Application notes
Self-contained unit for faster, more convenient transfers
The iBlot 2 Dry Blotting System was designed to deliver a high degree of convenience. The iBlot 2 Dry Blotting System is a true self-contained, plug-and-play system, with an integrated power supply, pre-packaged ready-to-use transfer stacks. Setup, teardown, and cleanup only takes minutes compared to the much more cumbersome and hands-on steps required for wet-tank transfer. The iBlot 2 Dry Blotting System has a simple touchscreen interface and pre-programmed optimized transfer programs, with the option to customize programs to suit your needs.
High transfer efficiency
In addition to ease of use and convenience, the iBlot 2 Dry Blotting System offers high-efficiency protein transfers for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, it helps you achieve more accurate detection using less sample. Transfer proteins in 7 minutes and move on quickly to the rest of your western blot processing.
Fits your gel size and works with your gel chemistry
The iBlot 2 Dry Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size (8 x 13 cm), E-PAGE 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm). The system is also compatible with all protein gel chemistries (tris-gylcine, bis-tris, tris-acetate, and tricine).
| Specifications | |
|---|---|
| Capacity | 1-2 mini, or 1 midi, or 1 E-PAGE gel |
| Compatibility | 1.0-1.5 mm thick gels |
| Transfer time | 6-10 min |
| Transfer buffer | No buffer required |
| Programs | Pre-programmed with six methods for transfer of proteins from various gel types or create custom programs |
| Power supply | Internal (no additional power supply needed) |
| Dimensions | 37 x 20 x 11 cm |
| Weight | 2.3 kg |
| Electrical Parameters | 100-240 V, 50/60 Hz, 6.3 A |
The iBlot 2 Dry Blotting System is based on the dry blotting concept, utilizing a unique gel matrix technology. The iBlot 2 Transfer Stack consists of a Bottom Stack and a Top Stack sandwiching a pre-run gel and a nitrocellulose (0.2 µm) or PVDF (0.2 µm) membrane. The iBlot 2 Transfer Stack is assembled with the blotting membrane on the anode side, and a pre-run gel on the cathode side.
Schematic of iBlot 2 Transfer Stack showing the flow of current |
|
Complete transfer of proteins from the gel to the blotting membrane is accomplished in approximately 7–8 minutes. The rapid transfer without the need for external power supply or premade buffers is possible due to the following features of the iBlot 2 Dry Blotting System:
- The gel matrix of the Bottom and Top Stack incorporate the appropriate anode and cathode buffers to act as ion reservoirs. This format eliminates the need for premade buffers or soaked filter paper, and minimizes handling that can lead to inconsistent performance.
- The copper anode does not generate oxygen gas as a result of water electrolysis, resulting in increased transfer consistency. Conventional inert electrodes present in other blotting systems result in oxygen generation, which can result in blotting distortion.
- The design of the iBlot 2 Gel Transfer Device reduces the distance between the electrodes and the integrated power supply. This unique design combined with the gel matrix technology of iBlot 2 Transfer Stacks allows the system to generate high field strength and increase the transfer speed.
How to use the iBlot 2 Dry Blotting System
Learn how to transfer your proteins from polyacrymide gels to nitrocellulose or PDVF membranes in a little as 7 minutes with this detailed step-by-step video.
Get high efficiency transfers in a fraction of the time
A successful western blot experiment relies heavily on the transfer efficiency of proteins from your gel to the blotting membrane. Traditional wet transfer offers high efficiency, but at the cost of significant hands-on processing time and transfer time. iBlot 2 Dry Blotting System offers the same high quality transfer but with substantial improvements in speed and convenience.
Chemiluminescent detection
High transfer efficiencies achieved using the iBlot Dry Blotting System. A431 lystate was separated on Novex Tris-glycine gels and transferred to nitrocellulose using a traditional wet tank system or the iBlot 2 Dry Blotting System. The membrane was probed for EGFR, Hsp90, PDI and p23 and detected using SuperSignal West Dura Extended duration chemiluminescent substrate.
Multiplex fluorescent detection
High transfer efficiencies achieved using the iBlot Dry Blotting System. A431 lystate was separated on NuPAGE Bis-Tris gels and transferred to nitrocellulose using a traditional wet tank system or the iBlot 2 Dry Blotting System. The membrane was probed for EGFR, Hsp90, tubulin and p23.
Efficient transfers across various protein gel chemistries
The iBlot 2 Gel transfer devices works with various protein gel chemistries providing uniformity and efficiency in protein transfer.
|
Get a jumpstart and save with our starter kits!
Our starter kits include everything you need to start transferring your proteins with the Invitrogen iBlot 2 Gel Transfer Device.
Option 1: nitrocellulose membranes
Includes:
- iBlot 2 Gel Transfer Device
- iBlot 2 Transfer Stacks, nitrocellulose, regular size (2 boxes, 20 stacks)
Option 2: PVDF membranes
Includes:
- iBlot 2 Gel Transfer Device
- iBlot 2 Transfer Stacks, PVDF, regular size (2 boxes, 20 stacks)
Still using your original iBlot device? Upgrade to an iBlot2 Dry Blotting System Today.
Manuals
Application notes
For Research Use Only. Not for use in diagnostic procedures.
