The revolutionary iBlot Dry Blotting System just got better

Introducing the improved Invitrogen iBlot 2 Dry Blotting System. Enjoy fast western transfer without sacrificing efficiency and uniformity. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. Now, with a new touch screen, less consumable waste, and sturdy design, fast western transfer never looked better.

Transfer proteins quickly and efficiently from gel to membrane in 7 minutes or less

  • High detection sensitivity and evenness
  • Increased blotting reliability and reproducibility
  • Flexible gel-size formats and membrane types
  • Simple, user-friendly system
  • Ability to create new custom programs
  • Built-in tutorial and application notes
  • High-quality and more compact transfer stacks

 Application Note—iBlot 2 High Molecular Weight Protein Transfer

How to use the iBlot 2 Dry Blotting System

Comparison of western transfer systems

iBlot® 2 Gel Transfer DeviceComparison of the iBlot 2 Dry Blotting System with conventional semi-dry and wet transfer systems.

iBlot 2 Dry Blotting System technical resources

iBlot® 2 Gel Transfer DeviceRegister your iBlot 2 Dry Blotting System, find FAQs, and download the manual and quick reference card. 

Resources for the original iBlot Gel Transfer Device

Original iBlot® Gel Transfer DeviceFind application notes, FAQs, literature citations and more for the original Invitrogen iBlot Gel Transfer Device.

Save when you order the Bolt Gel Welcome Pack together with an iBlot 2 Dry Blotting System

Bolt Gel Welcome Pack

This special package offers everything you need for the entire workflow, from protein separation to western blotting. With the Invitrogen Bolt Gel Welcome Pack with iBlot 2 Dry Blotting System, you’ll receive:

  • iBlot 2 Gel Transfer Device
  • iBlot 2 Nitrocellulose Regular Stacks (10 stacks)
  • Mini Gel Tank
  • Bolt 4–12% Bis-Tris Plus Gel, 10 Well (10 gels)
  • Bolt buffers and reagent

Order the Welcome Pack

Faster, more efficient protein transfer

Self-contained unit for faster, more convenient transfers

The iBlot 2 Dry Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. A self-contained unit, the iBlot device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes, offering the convenience of a bufferless, plug-and-play system.

High transfer efficiency

In addition to ease and convenience, the iBlot system offers high-efficiency protein transfers for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, you’ll achieve more accurate detection using less sample.

Fits your gel size

The iBlot Dry Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size, fitting Invitrogen Bolt Bis-Tris Plus gels, Novex Midi Gels (8 x 13 cm), E-PAGE 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm).

Resources

For Research Use Only. Not for use in diagnostic procedures.