iBlot® 2 Dry Blotting System  

Find the quick reference card, instruction manual, and FAQs for the iBlot® 2 Gel Transfer Device, and be sure to register your device so that you can receive the latest product information including firmware updates.

If you are looking for technical resources for the original iBlot® device, see Reagents and Resources for the Original iBlot® Gel Transfer Device.

Registration and firmware

Register your device to access the newest versions of firmware for the iBlot® 2 Gel Transfer Device as they become available.

Note for IE users: (1) right-click the above “Download” link; (2) click “Save Target As”; (3) Change file name to “image.ib2”; (4) Select file type as “All Files”; (5) click “Save”

iBlot2 firmware upgrade instructions

Frequently Asked Questions

Is the iBlot® 2 Gel Transfer Device compatible with original iBlot® Transfer Stacks?

No. Do not use iBlot® Transfer Stacks in the iBlot® 2 Gel Transfer Device, and do not mix components between iBlot® Transfer Stacks and iBlot® 2 Transfer Stacks.

Are consumables still available for the original iBlot® Gel Transfer Device?

Yes, all original iBlot® stacks are still available for purchase. You can find them in the original iBlot® Gel Transfer Device Reagents and Resources.

What is the shelf life of the iBlot® 2 Transfer Stacks?

It is currently 9 months, and the expiration date is printed on the box.

Can I use the stacks after the expiration date?

No. The results may be inferior after the expiration date.

Can the iBlot® 2 be used for transfer of low- and high–molecular weight proteins?

Yes. However, minor optimizations in the transfer protocol (i.e., steps, voltage, time) may be needed for some proteins.

Can I create my own transfer conditions?

Yes. The device allows programming custom methods.

Can I perform multiple transfers, with a new transfer starting immediately after the previous one?

Yes. The device is designed to do so with no impact on performance.

Can I use the stack and/or absorbent pad more than once?

No. They are disposable and should only be used once.

Are the iBlot® 2 PVDF and NC membranes compatible with fluorescent antibodies or membrane staining (e.g., LI-COR®, Ponceau S)?

The membranes are compatible with all commonly used detection methods such as staining, immunodetection, fluorescence, etc.

Can I replace the membrane with my preferred membrane?

It is possible but the results may be inferior.

Is the PVDF membrane pre-activated?

Yes. The PVDF membrane is pre-activated and ready for use without having to perform any pre-treatment with alcohol.

Can I cut the membrane to fit my separating gel?

No. Maintain the membrane size identical to the transfer stacks. This helps ensure that there is no direct contact between the top and bottom transfer stacks.

Can I cut the bottom stack to fit my separating gel?

No. Do not trim the iBlot® 2 Transfer Stacks to fit your gel size.

If a green color appears on the membrane after the transfer, will it affect the results?

No. It will not affect the western results.