Pierce Power System - FAQs
Power Station Equipment questions
Yes. The Pierce Power Station automatically recognizes the cassette that is inserted and initialized the correct software menu system.
No. It is not possible to insert the Power Blot Cassette and access the Power Staining protocol or vice versa.
No. Each cassette can only be used for its intended purpose; Power Blot Cassette for blotting (fast protein transfer) and Power Stain Cassette for protein staining. We have optimized the surface area to yield the best results for each application. The smaller stain cassette works better for staining while the larger blot cassette works better for blotting.
Power Stainer questions
Yes. The Pierce Power Blot Cassette (22835) can be purchased and will activate the pre-loaded Power Blotter software once inserted into your device.
Detection limits are similar to the commercially available protein stains (~0.01 to 50µg of pure protein).
The Power Stainer Cassette accommodates the staining for 1 or 2 mini gels or 1 midi gel.
Several commercially available precast gels were tested including NuPAGE Bis-Tris Mini and Midi Gels (Life Technologies), Bolt Bis-Tris Plus Mini Gels (Life Technologies), Novex Tris-Glycine Mini and Midi Gels (Life Technologies), Mini-Protean Mini Gels (Bio-Rad), Criterion HCl Midi Gels (Bio-Rad) and homebrew Tris-Glycine gels. Other gel types may be compatible but were not evaluated with this system and may be optimized using the custom staining programs.
Mini-sized gels with dimensions of 7x8.4mm and midi-sized gels 8x13.5cm are compatible with mini- and midi-sized gel pad. Gel thickness of 1mm is the most common. Gels with a thickness greater or less than 1mm would require time optimization.
Power Staining Kits have enough materials for 30 mini-sized gels or 15 midi-sized gels using the corresponding kit.
No. For the best results, use freshly prepared staining and destaining pads. Discard the used pads after each staining.
No. The consumable Mini and Midi Gel Pads (8 layers per pad) are required to be used with the Power Stainer, Power Stain Solution and Destain Solution. The gel pads act as reservoirs for Power Stain Solution and Destain Solution. Standard Western blotting filter paper will cause uneven staining and patchy background.
R-250 Coomassie dye is used. When optimized voltage is applied to the platinum-coated anode and stainless steel cathode, the negative charge of R-250 dye causes it to travel through the gel and bind to the proteins. Unbound dye continues through and out of the gel matrix to destain the background.
No. The system will not work with regular Coomassie stain.
No. Power Stainer requires optimized and proprietary Power Stain Solution and Destain Solution.
Yes. Just place the gel in the Coomassie stain for 30-60 minutes and destain according to the manufacturer's protocol.
Gels stained using the Power Stainer can be stored in a plastic protective sheet or in water for up to 4 hours without loss of signal.
Yes. It is compatible with mass spectrometry with similar results to conventional Coomassie staining.
Yes. The Pierce Power Stain Cassette (22836) can be purchased and will activate the pre-loaded Power Stainer software once inserted into your device.
No. During the staining the proteins are fixed to the gel thus the Western blotting results would not be optimal. The unstained gel should be used for Western blotting.
Yes. The Pierce G2 Fast Blotter is discontinued. The direct replacement for the Pierce G2 Fast Blotter is The Pierce Power Blotter (Part No. 22834). The 1-Step Transfer buffer (Part No. 84731) is still available.
The Pierce Power Blot Cassette (22835) is compatible with the Pierce G2 Fast Blotter.
We offer the Pierce G2 Blotter- Power Stainer Upgrade Kit. This kit contains the Pierce Power Stain Cassette and a USB Flash Drive loaded with software that will update the Pierce G2 Fast Blotter Control Unit software and add the staining functions.
For Research Use Only. Not for use in diagnostic procedures.