Wet Tank Transfer Systems

Wet transfer is the most common and traditional strategy for western blot transfer due to its efficiency and throughput. We offer two systems for tank transfer that minimize the amount of transfer buffer required.

Transfer membranes  Transfer buffers

Which wet tank transfer system is right for you?

  Invitrogen Mini Blot Module and Mini Gel Tank Invitrogen XCell II Blot Module and SureLock Tank
  Mini Blot Module XCell II Blot Module
Capacity 1-2 mini gels 1-2 mini gels
Transfer buffer requirements 220 mL per blot 800 mL
Transfer time 60 min 60-120 min
Blotting area 9 x 9 cm 9 x 9 cm
Compatible power supply PowerEase, Owl systems, for other systems use with Novex Power Supply Adapters (Cat. No. ZA10001) PowerEase, Owl systems, for other systems use with Novex Power Supply Adapters (Cat. No. ZA10001)
Required equipment Mini Gel Tank XCell SureLock Mini-Cell or XCell II Mini-Cell

Convenient, reliable western transfer

Two Mini Blot Modules fits into the Mini Gel Tank and is designed to make your western transfers simple and easy to perform.

  • Universal module design—allows modules to fit in either chamber of the tank, simplifying the transfer setup
  • Unique gasket seal—helps prevent buffer leakage so there is no mess during setup of your western transfer
  • 1/2 inch buffer chamber—requires half the typical volume of methanol-based transfer buffers
  • Standard 60 min transfer protocol—accelerates your western workflow so you can get results faster
  • Robust electrodes, sturdy steel plates—for highly efficient and reliable western transfers

Example transfer conditions

Gel type Membrane Voltage Starting current (mA) Ending current (mA) Run time
Bolt Bis-Tris Plus 4-12% (MES) Nitrocellulose 10 160 60 60
PVDF 20 340 130 60
NuPAGE 4-12% Bis-Tris (MES) Nitrocellulose 10 160 60 60
PVDF 20 390 130 60
Novex 4-20% Tris-Glycine (denatured) Nitrocellulose 10 70 50 60
PVDF 20 160 100 60
NuPAGE 3-8% Tris Acetate (denatured) Nitrocellulose 10 150 50 60
PVDF 20 380 130 60
Novex 10-20% Tricine Nitrocellulose 10 70 60 60
PVDF 20 180 150 60

* Current readings represent values when running a single gel, and can vary depending upon the power supply being used.

How-to-videos

How to perform a western wet tank transfer using the Invitrogen Mini Blot Module

Learn how to perform a western blot transfer using the Mini Blot module in a Mini Gel Tank.

How to separate proteins using an Invitrogen precast SDS-PAGE gel

Learn how to separate proteins by using a Invitrogen Bolt Bis-Tris Plus SDS-PAGE precast gel and Mini Gel Tank.

Get started with Mini Bolt Module Welcome Packs

The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for Western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.

Example transfer conditions

Gel type Membrane Transfer buffer Transfer conditions Expected current Run time
Tris-Glycine Nitrocellulose or PVDF Tris-Glycine Transfer Buffer with 20% methanol.

1X Transfer Buffer should be pH 8.3 before addition of SDS or methanol.
25V constant 100 mA 1-2 hr
Tricine Nitrocellulose or PVDF Tris-Glycine Transfer Buffer with 20% methanol.

1X Transfer Buffer should be pH 8.3 before addition of SDS or methanol.
25V constant Start: 100 mA 1-2 hr
Bis-Tris Nitrocellulose or PVDF Bis-Tris transfer buffer with 10% methanol and antioxidant for reduced samples 30 V constant Start: 170 mA End: 100mA 1 hour
Tris-Acetate Nitrocellulose or PVDF Bis-Tris transfer buffer with 10% methanol and antioxidant for reduced samples 30 V constant Start: 200 mA End: 180mA 60
IEF Nitrocellulose or PVDF 0.7% acetic acid, pH 3.0 10 V constant Start: 65-85 mA 60
TBE, TBE-Urea and DAN Retardation Nylon 45 mM Tris, 45 mM boric acid, 1 mM EDTA 30 V constant Start: 360 mA End: 270 mA 1-2 hr

The expected current listed in the table below is for transferring one gel. If you are transferring two gels in the blot module, the expected current will double.

For overnight blotting, transfer at a constant voltage of 10-15 V.

Discover how to use the XCell II Blot Module

Learn how to perform a wet tank transfer using the XCell II Blot Module in the SureLock Tank with this detailed step by step video.

Learn how to run a gel using the XCell SureLock System

Watch this step by step video of how to run a NuPAGE precast get in the SureLock Tank.

Which wet tank transfer system is right for you?

  Invitrogen Mini Blot Module and Mini Gel Tank Invitrogen XCell II Blot Module and SureLock Tank
  Mini Blot Module XCell II Blot Module
Capacity 1-2 mini gels 1-2 mini gels
Transfer buffer requirements 220 mL per blot 800 mL
Transfer time 60 min 60-120 min
Blotting area 9 x 9 cm 9 x 9 cm
Compatible power supply PowerEase, Owl systems, for other systems use with Novex Power Supply Adapters (Cat. No. ZA10001) PowerEase, Owl systems, for other systems use with Novex Power Supply Adapters (Cat. No. ZA10001)
Required equipment Mini Gel Tank XCell SureLock Mini-Cell or XCell II Mini-Cell

Convenient, reliable western transfer

Two Mini Blot Modules fits into the Mini Gel Tank and is designed to make your western transfers simple and easy to perform.

  • Universal module design—allows modules to fit in either chamber of the tank, simplifying the transfer setup
  • Unique gasket seal—helps prevent buffer leakage so there is no mess during setup of your western transfer
  • 1/2 inch buffer chamber—requires half the typical volume of methanol-based transfer buffers
  • Standard 60 min transfer protocol—accelerates your western workflow so you can get results faster
  • Robust electrodes, sturdy steel plates—for highly efficient and reliable western transfers

Example transfer conditions

Gel type Membrane Voltage Starting current (mA) Ending current (mA) Run time
Bolt Bis-Tris Plus 4-12% (MES) Nitrocellulose 10 160 60 60
PVDF 20 340 130 60
NuPAGE 4-12% Bis-Tris (MES) Nitrocellulose 10 160 60 60
PVDF 20 390 130 60
Novex 4-20% Tris-Glycine (denatured) Nitrocellulose 10 70 50 60
PVDF 20 160 100 60
NuPAGE 3-8% Tris Acetate (denatured) Nitrocellulose 10 150 50 60
PVDF 20 380 130 60
Novex 10-20% Tricine Nitrocellulose 10 70 60 60
PVDF 20 180 150 60

* Current readings represent values when running a single gel, and can vary depending upon the power supply being used.

How-to-videos

How to perform a western wet tank transfer using the Invitrogen Mini Blot Module

Learn how to perform a western blot transfer using the Mini Blot module in a Mini Gel Tank.

How to separate proteins using an Invitrogen precast SDS-PAGE gel

Learn how to separate proteins by using a Invitrogen Bolt Bis-Tris Plus SDS-PAGE precast gel and Mini Gel Tank.

Get started with Mini Bolt Module Welcome Packs

The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for Western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.

Example transfer conditions

Gel type Membrane Transfer buffer Transfer conditions Expected current Run time
Tris-Glycine Nitrocellulose or PVDF Tris-Glycine Transfer Buffer with 20% methanol.

1X Transfer Buffer should be pH 8.3 before addition of SDS or methanol.
25V constant 100 mA 1-2 hr
Tricine Nitrocellulose or PVDF Tris-Glycine Transfer Buffer with 20% methanol.

1X Transfer Buffer should be pH 8.3 before addition of SDS or methanol.
25V constant Start: 100 mA 1-2 hr
Bis-Tris Nitrocellulose or PVDF Bis-Tris transfer buffer with 10% methanol and antioxidant for reduced samples 30 V constant Start: 170 mA End: 100mA 1 hour
Tris-Acetate Nitrocellulose or PVDF Bis-Tris transfer buffer with 10% methanol and antioxidant for reduced samples 30 V constant Start: 200 mA End: 180mA 60
IEF Nitrocellulose or PVDF 0.7% acetic acid, pH 3.0 10 V constant Start: 65-85 mA 60
TBE, TBE-Urea and DAN Retardation Nylon 45 mM Tris, 45 mM boric acid, 1 mM EDTA 30 V constant Start: 360 mA End: 270 mA 1-2 hr

The expected current listed in the table below is for transferring one gel. If you are transferring two gels in the blot module, the expected current will double.

For overnight blotting, transfer at a constant voltage of 10-15 V.

Discover how to use the XCell II Blot Module

Learn how to perform a wet tank transfer using the XCell II Blot Module in the SureLock Tank with this detailed step by step video.

Learn how to run a gel using the XCell SureLock System

Watch this step by step video of how to run a NuPAGE precast get in the SureLock Tank.

Resources