The Protein Analysis Learning Center is here to assist you in your quest to understand the proteome, whether you want to review the basics, gain more in-depth knowledge, or discover how to use the latest research tools. Find resources for the different protein analysis techniques starting from protein expression to quantitative mass spectrometry including webinars, articles, protocols, and troubleshooting tips.
Protein sample preparation
Learn about methods and technologies for efficient cell lysis, protein extraction and fractionation, and targeted inhibition of unwanted protease and phosphatase activity.
Protein electrophoresis and western blotting
Learn about methods and technologies for separation and analysis of proteins by polyacrylamide gel electrophoresis (PAGE) from the different gel chemistries to buffer recipes. Find protocols, troubleshooting tips, and webinars to achieve publication quality western blot detection.
Protein and antibody purification
Learn about the different methods and tools for protein purification from affinity purification, ion-exchange chromatography, resins, beads, and support to purify your protein of interest.
Learn about methods and technologies for peptide and protein analysis by high performance liquid chromatography and mass spectrometry (LC/MS). Understand sample prep tools, mass and isotope labeling strategies for quantitative proteomics, and the basics of MS data and analysis.
Protein modification and crosslinking
Learn about methods and technologies for protein labeling, modification, and crosslinking. Understand the chemical functional groups and reaction chemistries required to crosslink and conjugate proteins to one another; to label proteins with biotin, PEG, or fluorescent dyes; or to reduce, cleave, and fragment proteins for use in assays.
Learn about methods and technologies for protein interaction and activity assays, enzyme reporter assays, and immunoprecipitation and pull-down tools.
Discover our other Learning Centers
Dive into deeper knowledge of life science techniques by exploring our Learning Centers, rich with resources by topic.
Resources & support
For Research Use Only. Not for use in diagnostic procedures.