Start here to learn about or increase your knowledge of protein biology

Protein biology encompasses both the study of the structure and function of proteins as the primary focus of investigation and the use of antibodies, proteins and peptides as tools to purify, detect, and characterize biological systems. Certain methods of protein analysis, such as western blotting and ELISA, have been used routinely in laboratories for many years. Others, such as quantitative protein mass spectrometry, are relatively recent and rapidly developing technologies. New tools and products are continually being developed to advance all aspects of protein biology research.

The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about protein analysis methods and products by providing a few key points of entry into the vast content.
Western Blot Video Tutorials

Videos that demonstrate each step in the western blot workflow, including SDS-PAGE, transfer, blocking, probing with antibodies, chemiluminescent detection, and stripping the blot.

Overview of Mass Spectrometry

A review of the history, applications and methodology of mass spectrometry, including ionization (ESA, MALDI), mass analyzers (TOF, quadrupole, ion trap), ion detectors, MS/MS, LC-MS/MS and sample prep.


ELISA Enzyme Substrates Selection Guide

This guide provides the general information necessary for selecting an approriate substrate and choosing initial primary and secondary antibody dilutions.


Pierce Protein Methods

Pierce™ Protein Methods library is an encyclopedia of articles about protein analysis laboratory techniques. The continually expanded and updated library is a valued resource for new and experienced researchers alike.

Protein Biology Application Notes

Protein Biology Application Notes are journal-style articles that report on research applications and proof-of-concept experiments with various products from our vast portfolio.

Protein Biology Selection Guides

This library of Protein Biology Selection Guides is a menu of links to various web pages that provide comparison information and selector tools to help you choose among specific products of various kinds.

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