Antibodies and Proteins Information

Learn about methods and technologies for preparing and using antibodies and recombinant proteins in protein analysis techniques. Understand how specific primary and secondary antibodies are produced, screened, purified, and modified to enable their use in a myriad of protein detection (identification and measurement) applications.

Primary antibodies
Secondary antibodies
Antibody production
Immunogen preparation
Antibody purification
Antibody labeling

ELISA
Multiplex assays
Microarrays
Immunoassays
Immunoprecipitation
Western blotting

Technical handbooks
Protein methods articles
Product application notes
Support center resources
Protocols
Product selection guides

Antibodies and proteins features

Antibodies Learning Center

The Antibodies Learning Center organizes education information about antibodies. Antibody Methods reviews the steps of antibody production and purification. Cell Signaling Pathways provides visual maps of protein-mediated cellular processes, together with links to antibody search results.

How to Choose and Select a Secondary Antibody

Considerations for choosing a secondary antibody, including host and target species, targeted reactivity, purification, cross-adsorption, multiplexing, antibody class and subclass, whole antibodies vs. fragments, conjugates, and biotin-binding proteins.

Antibodies and proteins learning resources

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Type	Title
Handbook	Antibody-Based Tools for Evaluating Tumor-Related Inflammation (2016)
Handbook	Antibody-Based Tools for Neurodegenerative Disease Research (2015)
Handbook	Antibodies for Stem Cell Research (2015)
Pathways	Cell Signaling Pathways (visual maps and antibody target search)
Methods article	Antibody Methods Library
Methods article	Introduction to Immunoglobulins
Methods article	Introduction to Antibody Production and Purification
Methods article	Antibody Production (Immunogen Preparation)
Methods article	Antibody Isotyping and Characterization Methods
Methods article	Antibody Purification Methods
Methods article	Antibody Fragmentation
Methods article	Immunoglobulin Structure and Classes
Methods article	Antibody Labeling and Immobilization Sites
Methods article	Antibody IgG Binding Proteins
Methods article	Protein Methods Library
Methods article	An Overview of Detection Probes
Methods article	Secondary Antibodies as Probes
Methods article	Enzyme Probes
Methods article	Avidin-Biotin Interaction
Methods article	Fluorescent Probes
Methods article	Peptide Design
Methods article	Peptide Synthesis
Methods article	Amino Acid Physical Properties
Selection guide	Primary Antibodies (Selection Tool)
Selection guide	Secondary Antibodies (Selection Tool)
Selection guide	How to Choose and Select a Secondary Antibody
Selection guide	Immunoassays (Selection Guide)
Selection guide	Find ELISA Kits by Target (Selection Guide)
Application note	Protein Biology Application Notes
Application note	Antibody Application Notes
Application note	Characterization of antibody specificity using immunoprecipitation and mass spectrometry (IP-MS antibody validation).
Application note	A step-by-step guide to successful chromatin immunoprecipitation (ChIP) assays and antibody selection.
Application note	ABfinity rabbit monoclonal antibodies white paper.
Application note	Carrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity.
Application note	Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale.

Protein Biology Resource Library

Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.