Antibodies and Proteins Information

Learn about methods and technologies for preparing and using antibodies and recombinant proteins in protein analysis techniques. Understand how specific primary and secondary antibodies are produced, screened, purified, and modified to enable their use in a myriad of protein detection (identification and measurement) applications.

  • Primary antibodies
  • Secondary antibodies
  • Antibody production
  • Immunogen preparation
  • Antibody purification
  • Antibody labeling 
  • ELISA
  • Multiplex assays
  • Microarrays
  • Immunoassays
  • Immunoprecipitation
  • Western blotting
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Support center resources
  • Protocols
  • Product selection guides

Antibodies and proteins features

The Antibodies Learning Center organizes education information about antibodies. Antibody Methods reviews the steps of antibody production and purification. Cell Signaling Pathways provides visual maps of protein-mediated cellular processes, together with links to antibody search results.

Considerations for choosing a secondary antibody, including host and target species, targeted reactivity, purification, cross-adsorption, multiplexing, antibody class and subclass, whole antibodies vs. fragments, conjugates, and biotin-binding proteins.


Antibodies and proteins learning resources

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TypeTitle
HandbookAntibody-Based Tools for Evaluating Tumor-Related Inflammation (2016)
HandbookAntibody-Based Tools for Neurodegenerative Disease Research (2015)
HandbookAntibodies for Stem Cell Research (2015)
PathwaysCell Signaling Pathways (visual maps and antibody target search)
Methods articleAntibody Methods Library
Methods articleIntroduction to Immunoglobulins
Methods articleIntroduction to Antibody Production and Purification
Methods articleAntibody Production (Immunogen Preparation)
Methods articleAntibody Isotyping and Characterization Methods
Methods articleAntibody Purification Methods
Methods articleAntibody Fragmentation
Methods articleImmunoglobulin Structure and Classes
Methods articleAntibody Labeling and Immobilization Sites
Methods articleAntibody IgG Binding Proteins
Methods articleProtein Methods Library
Methods articleAn Overview of Detection Probes
Methods articleSecondary Antibodies as Probes
Methods articleEnzyme Probes
Methods articleAvidin-Biotin Interaction
Methods articleFluorescent Probes
Methods articlePeptide Design
Methods articlePeptide Synthesis
Methods articleAmino Acid Physical Properties
Selection guidePrimary Antibodies (Selection Tool)
Selection guideSecondary Antibodies (Selection Tool)
Selection guideHow to Choose and Select a Secondary Antibody
Selection guideImmunoassays (Selection Guide)
Selection guideFind ELISA Kits by Target (Selection Guide)
Application noteProtein Biology Application Notes
Application noteAntibody Application Notes
Application noteCharacterization of antibody specificity using immunoprecipitation and mass spectrometry (IP-MS antibody validation).
Application noteA step-by-step guide to successful chromatin immunoprecipitation (ChIP) assays and antibody selection.
Application noteABfinity rabbit monoclonal antibodies white paper.
Application noteCarrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity.
Application noteUsing antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale.