An encyclopedia of articles about protein analysis laboratory techniques

Pierce Protein Methods Library

Originally created to support Thermo Scientific Pierce products, this continually expanded and updated library is a valued resource for new and experienced researchers alike. Are you new to the protein biology laboratory and need a basic introduction to protein analysis techniques? Are you a seasoned scientist considering how to optimize an experiment involving protein expression, purification or detection? Pierce Protein Methods can help you. In addition, this educational content will assist you in choosing appropriate products from our portfolio to successfully accomplish your experiments.


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Protein Method Article TitleProtein Method CategoriesSubtopics
An Overview of Cell Lysis and Protein ExtractionCell Lysis and FractionationProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Traditional Methods of Cell LysisCell Lysis and FractionationProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Detergents for Cell Lysis and Protein ExtractionCell Lysis and FractionationProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Cell Lysis SolutionsCell Lysis and FractionationProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Cell Fractionation and Organelle IsolationCell Lysis and FractionationProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Protease and Phosphatase InhibitorsCell Lysis and FractionationProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
An Overview of Affinity PurificationProtein PurificationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Covalent Immobilization of Affinity LigandsProtein PurificationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
GST-tagged Proteins–Production and PurificationProtein PurificationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
His-tagged Proteins–Production and PurificationProtein PurificationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Overview of the Immunoprecipitation (IP) TechniqueProtein PurificationProtein Isolation and Purification, Protein Assays and Analysis,
An Overview of Buffer ExchangeDialysis, Desalting, and ConcentrationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Dialysis Methods for Protein ResearchDialysis, Desalting, and ConcentrationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Desalting and Gel Filtration ChromatographyDialysis, Desalting, and ConcentrationProtein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
An Overview of Protein AssaysProtein AssaysProtein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Chemistry of Protein AssaysProtein AssaysProtein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Protein Assay Data AnalysisProtein AssaysProtein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Protein-Protein Variation of Protein AssaysProtein AssaysProtein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
An Overview of ElectrophoresisProtein Gel ElectrophoresisProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis,
Protein Gel StainsProtein Gel ElectrophoresisProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting
An Overview of Detection ProbesDetection Probes, ELISA, Western BlottingWestern Blotting, Protein Assays and Analysis,
Introduction to Secondary AntibodiesDetection Probes, ELISA, Western BlottingWestern Blotting, Protein Assays and Analysis, Antibodies and Proteins
Enzyme ProbesDetection Probes, ELISA, Western BlottingWestern Blotting, Protein Assays and Analysis, Antibodies and Proteins
Avidin-Biotin InteractionDetection Probes, ELISA, Western Blotting, Protein PurificationWestern Blotting, Protein Assays and Analysis, Antibodies and Proteins
Fluorescent ProbesDetection Probes, ImmunohistochemistryWestern Blotting, Protein Assays and Analysis, Antibodies and Proteins
Luciferase ReportersReporter Gene AssaysProtein Assays and Analysis
An Overview of Western BlottingWestern BlottingProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Chemiluminescent Western BlottingWestern BlottingProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Stripping and Reprobing Western BlotsWestern Blotting, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis,
Fluorescent Western BlottingWestern BlottingProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Blocking Buffers for Western Blot and ELISAWestern BlottingProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Western Blot Transfer MethodsWestern BlottingProtein Gel Electrophoresis, Western Blotting,
Chromogenic Western Blotting SubstratesWestern BlottingProtein Gel Electrophoresis, Western Blotting
Western Blot Video TutorialsWestern BlottingProtein Gel Electrophoresis, Western Blotting
An Overview of ELISAELISAProtein Assays and Analysis, Antibodies and Proteins
ELISA Development and OptimizationELISAProtein Assays and Analysis, Antibodies and Proteins
Spike-and-Recovery and Linearity-of-Dilution Assessment for ELISAELISAProtein Assays and Analysis, Antibodies and Proteins
Factors Affecting Signal Generation in ELISAELISAProtein Assays and Analysis, Antibodies and Proteins
ELISA Troubleshooting GuideELISAProtein Assays and Analysis, Antibodies and Proteins
ELISA Data AnalysisELISAProtein Assays and Analysis, Antibodies and Proteins
An Overview of ImmunohistochemistryImmunohistochemistryAntibodies and Proteins
Immunohistochemistry vs ImmunocytochemistryImmunohistochemistryAntibodies and Proteins
Fixation Strategies and FormulationsImmunohistochemistryAntibodies and Proteins
Paraffin Removal and Antigen RetrievalImmunohistochemistryAntibodies and Proteins
Methods to Block Endogenous DetectionImmunohistochemistryAntibodies and Proteins
Blocking Strategies for IHCImmunohistochemistryAntibodies and Proteins
IHC ImmunodetectionImmunohistochemistryAntibodies and Proteins
Avidin-Biotin Complex Method for IHC DetectionImmunohistochemistryAntibodies and Proteins
Immunofluorescence Method for IHC DetectionImmunohistochemistryAntibodies and Proteins
IHC CounterstainsImmunohistochemistryAntibodies and Proteins
IHC Troubleshooting GuideImmunohistochemistryAntibodies and Proteins
An Overview of Protein-Protein Interaction AnalysisProtein-Protein InteractionsProtein Isolation and Purification, Protein Modification and Crosslinking
Co-Immunoprecipitation (Co-IP)Protein-Protein InteractionsProtein Isolation and Purification, Protein Modification and Crosslinking
Pull-Down AssaysProtein-Protein InteractionsProtein Isolation and Purification, Protein Modification and Crosslinking
Crosslinking Protein Interaction AnalysisProtein-Protein InteractionsProtein Modification and Crosslinking, Protein Modification and Crosslinking
Label Transfer Protein Interaction AnalysisProtein-Protein InteractionsProtein Isolation and Purification, Protein Modification and Crosslinking
Far-Western Blot AnalysisProtein-Protein InteractionsProtein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,  Protein Modification and Crosslinking
An Overview of Protein-Nucleic Acid InteractionsProtein-Nucleic Acid InteractionsProtein Modification and Crosslinking
Gel Shift Assays (EMSA)Protein-Nucleic Acid InteractionsProtein Modification and Crosslinking
Methods for Detecting Protein-DNA InteractionsProtein-Nucleic Acid InteractionsProtein Modification and Crosslinking
Methods for Detecting Protein-RNA InteractionsProtein-Nucleic Acid InteractionsProtein Modification and Crosslinking
Chromatin IP (ChIP Assays)Protein-Nucleic Acid InteractionsProtein Modification and Crosslinking
Methods for Labeling Nucleic AcidsProtein-Nucleic Acid InteractionsProtein Modification and Crosslinking
An Overview of Mass SpectrometryProtein Mass Spectrometry AnalysisProtein Isolation and Purification, Protein Mass Spectrometry
Quantitative ProteomicsProtein Mass Spectrometry AnalysisProtein Isolation and Purification, Protein Mass Spectrometry
Sample Preparation for Mass SpectrometryProtein Mass Spectrometry AnalysisProtein Isolation and Purification, Protein Mass Spectrometry
An Overview of Protein LabelingProtein LabelingProtein Isolation and Purification, Protein Modification and Crosslinking
BiotinylationProtein LabelingProtein Isolation and Purification, Protein Modification and Crosslinking
Metabolic Labeling and Chemoselective LigationProtein LabelingProtein Isolation and Purification, Protein Modification and Crosslinking
An Overview of Crosslinking and Protein ModificationProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Chemistry of CrosslinkingProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Crosslinking ApplicationsProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Polyethylene Glycol (PEG) and Pegylation of ProteinsProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Carbodiimide Crosslinker ChemistryProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Amine-Reactive Crosslinker ChemistryProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Sulfhydryl-Reactive Crosslinker ChemistryProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Carbonyl-Reactive Crosslinker ChemistryProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Photoreactive Crosslinker ChemistryProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
Staudinger Ligation Reaction ChemistryProtein CrosslinkingProtein Isolation and Purification, Protein Modification and Crosslinking
An Overview of Antibody Production and PurificationAntibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody Production (Immunogen Preparation)Antibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody Isotyping and Characterization MethodsAntibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody Purification MethodsAntibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody IgG Binding ProteinsAntibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody FragmentationAntibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody Structure and ClassesAntibody Production and PurificationProtein Expression, Antibodies and Proteins
Antibody Labeling and Immobilization SitesAntibody Production and PurificationProtein Expression, Antibodies and Proteins
An Overview of Post-Translational ModificationPost-Translational ModificationProtein Expression
PhosphorylationPost-Translational ModificationProtein Expression
GlycosylationPost-Translational ModificationProtein Expression
An Overview of Protein Expression SystemsProtein ExpressionProtein Expression
Cell-Free Protein ExpressionProtein ExpressionProtein Expression
Peptide DesignPeptidesProtein Expression
Peptide SynthesisPeptidesProtein Expression
Amino Acid Physical PropertiesPeptidesProtein Expression