Western blot analysis is one of the most common methods used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample and revealing their activity and any post-translational modifications. Western blotting is a multi-step process that can take between 1 to 2 days to complete, depending on the methods used. Below are videos that detail the common steps used to perform a western blot, from separating proteins in a lysate to developing the blot.
View and select products
- Protein Gel Electrophoresis
- Mini Gel Tanks
- Protein Standards and Ladders
- Transfer Proteins for Western Blot
- Membranes and Transfer Buffers for Western Blotting
- Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes
- Novex Reversible Membrane Protein Stain Kit
- Western Blot Protein Detection
- SuperSignal Western Blot Enhancer
- iBind Western Systems
- Pierce Power Blotter
Western blot video tutorials
Watch this video on separating proteins by SDS-PAGE
Watch this video on moving proteins from gel to membrane
Watch this video on performing a semi-dry transfer of proteins from gel to membrane
Watch this video on performing dry transfer of proteins from gel to membrane
Watch this video on performing hands-free western detection
Watch this video on reducing background signal
Watch this video on finding your target proteins
Watch this video on ID targeting with chemiluminescence
Watch this video on preparing blot to reprobe
- Overview of Protein Electrophoresis
- Western Blot Transfer Methods
- Blocking Buffers for Western Blotting and ELISA
- Overview of Western Blotting
- Chemiluminescent Western Blotting
- Stripping and Reprobing Western Blots
- Protein Research Handbook
- Protein Electrophoresis and Western Blotting Support Center
- Coligan, J.E., et al., Eds. (2002) Electrophoresis. In: Current Protocols in Protein Science. New York: John Wiley and Sons, Inc. pp 10.0.1–10.4.36.
- Bollag, D.M., Rozycki, M.D. and Edelstein, S.J. (2002) Protein Methods, 2nd ed. New York: Wiley-Liss, Inc.
- Hames, B.D. and Rickwood, D. Eds. (1990) Gel Electrophoresis of Proteins: a Practical Approach, 2nd ed. New York: Oxford University Press.
- Laemmli, U.K. (1970) Nature 227:680–685. PMID: 5432063
For Research Use Only. Not for use in diagnostic procedures.