Western blot analysis is one of the most common methods used in the lab today, because of its versatility in detecting and measuring specific target proteins in a sample and revealing their activity and any post-translational modifications. Western blotting is a multi-step process that can take 1-2 days to complete, depending on the methods used. Below are videos that detail the common steps to perform a Western blot, from separating proteins in a lysate to developing the blot.


Watch this video on separating proteins by SDS-PAGE

Watch this video on moving proteins from gel to membrane

Watch this video on reducing background signal

Watch this video on finding your target proteins

Watch this video on ID targeting with chemiluminescence


Watch this video on preparing blot to reprobe