Western blot analysis is one of the most common methods used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample and revealing their activity and any post-translational modifications. Western blotting is a multi-step process that can take between 1 to 2 days to complete, depending on the methods used. Below are videos that detail the common steps used to perform a western blot, from separating proteins in a lysate to developing the blot.

Western blot video tutorials

Watch this video on separating proteins by SDS-PAGE


Watch this video on moving proteins from gel to membrane


Watch this video on performing a semi-dry transfer of proteins from gel to membrane


Watch this video on performing dry transfer of proteins from gel to membrane


Watch this video on performing hands-free western detection


Watch this video on reducing background signal


Watch this video on finding your target proteins


Watch this video on ID targeting with chemiluminescence


Watch this video on preparing blot to reprobe


Recommended reading

  1. Coligan, J.E., et al., Eds. (2002) Electrophoresis. In: Current Protocols in Protein Science. New York: John Wiley and Sons, Inc. pp 10.0.1–10.4.36.
  2. Bollag, D.M., Rozycki, M.D. and Edelstein, S.J. (2002) Protein Methods, 2nd ed. New York: Wiley-Liss, Inc.
  3. Hames, B.D. and Rickwood, D. Eds. (1990) Gel Electrophoresis of Proteins: a Practical Approach, 2nd ed. New York: Oxford University Press.
  4. Laemmli, U.K. (1970) Nature 227:680–685. PMID: 5432063