Journal-style articles that report on research applications and proof-of-concept experiments with various products from our vast portfolio.

Protein Biology Application Notes

The articles in the current library feature Thermo Scientific Pierce products and were first published on the legacy Pierce website. Do you want to read more about the experiments whose results are featured as tables or figures on individual product pages? Are you interested in learning how different reagents and instrumentation can be used in combination to investigate real-world research questions? You are likely to find such answers among these Application Notes, which are written and presented in an authored and citable journal-article format familiar to scientists.

Featured application notes

Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins

This article introduces the biology of Integral and peripheral membrane proteins (MPs) and their role in maintaining critical cellular functions. In addition, we describe the use of the Thermo Scientific Mem-PER Plus Membrane Protein Extraction Kit that enables small-scale solubilization and enrichment of integral and membrane-associated proteins from cultured cells and tissues.

An efficient method for the isolation of highly functional primary neurons

In this article, we report on the use of the Pierce Primary Neuron Isolation Kit method for generating high-yield, high-viability, high-quality neuronal cultures as manifested by their morphological characteristics, cell-specific protein expression, and the extent of synaptic scaling.

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Date Application note title Application note categories Featured product
2017-04-20 ProcartaPlex Human Immuno-Oncology Checkpoint Panel: detection of soluble isoforms of immuno-oncology checkpoint markers Multiplex Immunoassay ProcartaPlex Human Immuno-Oncology Checkpoint Panel
2016-09-20 Characterization of antibody specificity using immunoprecipitation and mass spectrometry (IP-MS antibody validation). Antibody Production, Mass Spectrometry, Immunoprecipitation Antibodies
2016-07-01 Optimizing protein A purification of monoclonal antibodies when using the ExpiCHO Expression System. Affinity Purification, Antibody Production ExpiCHO Expression System
2015-12-15 Guide to gels and transfer methods for high molecular weight proteins, featuring the Pierce Power Blotter. Electrophoresis and Staining, Western Blotting Pierce Power Blotter
2015-11-02 Pierce Power Stainer: a fast, reliable device for Coomassie protein staining and destaining. Electrophoresis and Staining, Western Blotting Pierce Power Stainer
2014-05-22 Transfer EMSA gels using the Pierce G2 Fast Blotter. The Pierce G2 Fast Blotter provides rapid semi-dry transfer of DNA (TBE) gels to nylon membrane. Electrophoresis and Staining, Western Blotting Pierce G2 Fast Blotter – Power Stainer Upgrade
2014-05-19 An improved method for highly efficient isolation of primary mouse embryonic fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts (MEF). Lysis and Extraction Pierce Mouse Embryonic Fibroblast Isolation Kit
2014-05-02 Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system. A cell-based, quantitative co-immunoprecipitation method for protein interaction analysis involving a sensitive luciferase assay strategy. Immunoprecipitation, Luciferase Assays Pierce Agarose qIP Protein Interaction Kit, Tluc and HA Tags
2014-04-02 One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts. Easily isolate and culture highly viable, functional primary cardiomyocytes. Lysis and Extraction Pierce Primary Cardiomyocyte Isolation Kit
2014-03-24 Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System. Protein Expression 1-Step Human Coupled IVT Kit - DNA
2014-03-24 Benchmarking experiments with Zeba Spin Desalting Columns. Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns. Dialysis and Desalting Zeba Micro Spin Desalting Columns, 7K MWCO, 75 µL
2014-03-03 Calculate the number of immobilized proteins per bead of agarose affinity supports. A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands. Affinity Purification AminoLink Plus Coupling Resin
2014-02-19 Improved, all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells. Lysis and Extraction B-PER Complete Bacterial Protein Extraction Reagent
2014-01-28 An efficient method for the isolation of highly functional primary neurons. Easily and reproducibly isolate and culture primary neurons. Lysis and Extraction Pierce Primary Neuron Isolation Kit
2014-01-16 Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria. Protein Expression 1-Step Human High-Yield Mini IVT Kit
2014-01-10 System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates. Protein Expression 1-Step Human High-Yield Mini IVT Kit
2013-11-12 Luciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers. Luciferase Assays Pierce Renilla Luciferase Flash Assay Kit
2013-10-22 Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system. Protein Expression, Fusion Tag Methods pT7CFE1-NHis Vector for Mammalian Cell-Free Protein Expression
2013-09-11 Analysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit. Immunoprecipitation, Molecular Biology Pierce Magnetic ChIP Kit
2013-08-29 Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins. Lysis and Extraction Mem-PER Plus Membrane Protein Extraction Kit
2013-08-19 Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents. Measure protein and peptide cysteine modifications using multiplex quantitative mass spectrometry. Mass Spectrometry, Post-translational Modification iodoTMTsixplex Label Reagent Set, 1 x 0.2 mg
2013-08-02 Performance characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins. Fusion Tag Methods, Affinity Purification Pierce Glutathione Superflow Agarose
2013-07-11 Performance characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins. Fusion Tag Methods, Affinity Purification HisPur Ni-NTA Superflow Agarose
2013-06-26 Improved Western blot detection using CCD digital imaging. Comparison of cooled-CCD digital imaging versus X-ray film for sensitivity, dynamic range and signal linearity with enhanced chemiluminescence (ECL). Western Blotting, Electrophoresis and Staining myECL Imager
2013-06-24 Versatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes. Luciferase Assays Pierce Gaussia Luciferase Flash Assay Kit
2013-06-05 Carrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity. Antibody Production Imject mcKLH (in PBS)
2013-06-04 A versatile mass spectrometry sample preparation procedure for complex protein samples. Prepare high-quality peptide digests with high recovery from cultured cells. Mass Spectrometry, Sample Preparation Pierce Mass Spec Sample Prep Kit for Cultured Cells
2013-05-22 Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates. Protein Expression 1-Step Human Coupled IVT Kit - DNA
2013-05-02 Performance characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin. Fusion Tag Methods, Affinity Purification HisPur Cobalt Superflow Agarose
2013-04-30 Activation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells. Luciferase Assays Pierce Gaussia Luciferase Flash Assay Kit
2013-04-18 Separation characteristics of dialysis membranes. Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing. Dialysis and Desalting Slide-A-Lyzer G2 Dialysis Cassettes, 10K MWCO, 3 mL
2013-03-08 Monitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging. Luciferase Assays, IHC and Cell Imaging Pierce Cypridina Luciferase Flash Assay Kit
2013-03-07 Chromatography cartridges for desalting and affinity purification. Data using the cartridge format for automated liquid chromatography (LC) systems. Affinity Purification, Dialysis and Desalting Zeba Micro Spin Desalting Columns, 7K MWCO, 75 µL
2013-01-24 Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader. Luciferase Assays, Protein Expression Pierce Cypridina-Firefly Luciferase Dual Assay Kit
2012-12-11 Quantitative cancer stem cell phosphoprotein profiling. Using tandem mass labeling and LC-MS/MS to profile perturbations of an autocrine loop. Mass Spectrometry, Post-translational Modification Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL
2012-11-12 Phosphoprotein enrichment from cell and tissue samples. Isolate phosphorylated proteins with an easy-to-use spin format Post-translational Modification, Affinity Purification Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL
2012-10-11 Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry. Mass Spectrometry 1-Step Heavy Protein IVT Kit
2012-07-18 Eliminate endotoxins from protein and antibody samples. A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges. Sample Preparation Pierce High Capacity Endotoxin Removal Resin
2012-07-09 Removing endotoxins using a spin-column format. Fast and effective removal of endotoxins from protein samples. Sample Preparation Pierce High Capacity Endotoxin Removal Resin
2012-06-13 Luciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency. Luciferase Assays Pierce Gaussia Luciferase Flash Assay Kit
2012-05-18 Fractionate subcellular proteins from tissue samples. Obtain proteins from five cellular components using a tissue-optimized kit. Lysis and Extraction Subcellular Protein Fractionation Kit for Tissues
2012-05-10 Method to isolate functional synaptosomes. Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity. Lysis and Extraction Syn-PER Synaptic Protein Extraction Reagent
2012-04-18 Complete protein protection from proteases and phosphatases. Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation. Lysis and Extraction, Sample Preparation Pierce Protease Inhibitor Tablets, EDTA-free
2012-03-05 Optimized protein extraction reagent for neuronal tissue and primary cells. Obtain high yields of functional neuronal protein from all neuronal cell compartments. Lysis and Extraction N-PER Neuronal Protein Extraction Reagent
2012-02-28 Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays. Luciferase Assays, Protein Expression Pierce Cypridina-Firefly Luciferase Dual Assay Kit
2012-02-24 Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale. Antibody Purification, Affinity Purification Pierce Protein A Plus Agarose
2011-10-07 Improved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer. Immunoprecipitation, Protein Immobilization GlycoLink IP Kit
2011-06-01 Western blotting using enhanced chemiluminescence. Choose the perfect substrate for your needs. Western Blotting Pierce ECL Western Blotting Substrate
2011-06-01 Serine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases. Protein Labeling, Protein Assays ActivX TAMRA-FP Serine Hydrolase Probe
2011-06-01 Selective enrichment of sialylated glycopeptides using TiO2 beads. Affinity purification of glycoproteins for mass spectrometry based on titanium dioxide beads (TiO2). Mass Spectrometry Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit
2011-06-01 Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications. Immunoprecipitation, Affinity Purification Pierce Protein A/G Magnetic Beads
2011-06-01 Optimize liquid chromatography with a peptide retention time calibration mixture. New mixture of 15 heavy peptides streamlines assay design for targeted peptide quantification. Chromatography, Mass Spectrometry Pierce Peptide Retention Time Calibration Mixture
2011-06-01 Calibration solutions for Thermo Scientific Mass Spectrometers. Ready-to-use standards enable quick instrument calibration. Mass Spectrometry Pierce LTQ ESI Positive Ion Calibration Solution
2011-06-01 New dimensions for low-volume sample dialysis. Self-contained devices give you the solution for high-protein recovery. Dialysis and Desalting, Sample Preparation Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL
2011-06-01 Introducing a Western blot substrate that is chemiluminescent and chemifluorescent. Detect your target by film, CCD cameras or laser-based imagers. Western Blotting Pierce ECL Plus Western Blotting Substrate
2011-06-01 Develop mid- to high-throughput SRM and MRM assays. New application-specific peptide libraries for quantitative mass spectrometry workflows. Peptides, Mass Spectrometry PEPotec SRM Custom Peptide Libraries
2011-04-05 Measure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates. Protein Assays, Immunoprecipitation Active Rac1 Pull-Down and Detection Kit
2011-04-05 GTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases. Protein Labeling, Affinity Purification Pierce GTPase Enrichment Kit with GTP Probe
2011-04-05 Enhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody. IHC and Cell Imaging Pierce Immunostain Enhancer
2011-04-05 Detection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies. IHC and Cell Imaging, Affinity Purification Active Rac1 Pull-Down and Detection Kit

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