Journal-style articles that report on research applications and proof-of-concept experiments with various products from our vast portfolio.

Protein Biology Application Notes

The articles in the current library feature Thermo Scientific Pierce products and were first published on the legacy Pierce website. Do you want to read more about the experiments whose results are featured as tables or figures on individual product pages? Are you interested in learning how different reagents and instrumentation can be used in combination to investigate real-world research questions? You are likely to find such answers among these Application Notes, which are written and presented in an authored and citable journal-article format familiar to scientists.


Featured application notes

Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins

This article introduces the biology of Integral and peripheral membrane proteins (MPs) and their role in maintaining critical cellular functions. In addition, we describe the use of the Thermo Scientific Mem-PER Plus Membrane Protein Extraction Kit that enables small-scale solubilization and enrichment of integral and membrane-associated proteins from cultured cells and tissues.

An efficient method for the isolation of highly functional primary neurons

In this article, we report on the use of the Pierce Primary Neuron Isolation Kit method for generating high-yield, high-viability, high-quality neuronal cultures as manifested by their morphological characteristics, cell-specific protein expression, and the extent of synaptic scaling.


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DateApplication note titleApplication note categoriesFeatured product
2020-07-27Extraction and Purification of Functional G Protein-Coupled Receptors from Expression SystemsCell membrane protein extraction and isolationGPCR Extraction and Stabilization Reagent
2017-04-20ProcartaPlex Human Immuno-Oncology Checkpoint Panel: detection of soluble isoforms of immuno-oncology checkpoint markersMultiplex ImmunoassayProcartaPlex Human Immuno-Oncology Checkpoint Panel
2016-09-20Characterization of antibody specificity using immunoprecipitation and mass spectrometryAntibody Production, Mass Spectrometry, ImmunoprecipitationAntibodies
2016-07-01Optimizing protein A purification of monoclonal antibodies when using the ExpiCHO Expression System.Affinity Purification, Antibody ProductionExpiCHO Expression System
2015-12-15Guide to gels and transfer methods for high molecular weight proteins, featuring the Pierce Power Blotter.Electrophoresis and Staining, Western BlottingPierce Power Blotter
2015-11-02Pierce Power Stainer: a fast, reliable device for Coomassie protein staining and destaining.Electrophoresis and Staining, Western BlottingPierce Power Stainer
2014-05-22Transfer EMSA gels using the Pierce G2 Fast Blotter. The Pierce G2 Fast Blotter provides rapid semi-dry transfer of DNA (TBE) gels to nylon membrane.Electrophoresis and Staining, Western BlottingPierce G2 Fast Blotter – Power Stainer Upgrade
2014-05-19An improved method for highly efficient isolation of primary mouse embryonic fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts (MEF).Lysis and ExtractionPierce Mouse Embryonic Fibroblast Isolation Kit
2014-05-02Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system. A cell-based, quantitative co-immunoprecipitation method for protein interaction analysis involving a sensitive luciferase assay strategy.Immunoprecipitation, Luciferase AssaysPierce Agarose qIP Protein Interaction Kit, Tluc and HA Tags
2014-04-02One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts. Easily isolate and culture highly viable, functional primary cardiomyocytes.Lysis and ExtractionPierce Primary Cardiomyocyte Isolation Kit
2014-03-24Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System.Protein Expression1-Step Human Coupled IVT Kit - DNA
2014-03-24Benchmarking experiments with Zeba Spin Desalting Columns. Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns.Dialysis and DesaltingZeba Micro Spin Desalting Columns, 7K MWCO, 75 µL
2014-03-03Calculate the number of immobilized proteins per bead of agarose affinity supports. A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands.Affinity PurificationAminoLink Plus Coupling Resin
2014-02-19Improved, all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells.Lysis and ExtractionB-PER Complete Bacterial Protein Extraction Reagent
2014-01-28An efficient method for the isolation of highly functional primary neurons. Easily and reproducibly isolate and culture primary neurons.Lysis and ExtractionPierce Primary Neuron Isolation Kit
2014-01-16Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria.Protein Expression1-Step Human High-Yield Mini IVT Kit
2014-01-10System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates.Protein Expression1-Step Human High-Yield Mini IVT Kit
2013-11-12Luciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers.Luciferase AssaysPierce Renilla Luciferase Flash Assay Kit
2013-10-22Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system.Protein Expression, Fusion Tag MethodspT7CFE1-NHis Vector for Mammalian Cell-Free Protein Expression
2013-09-11Analysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit.Immunoprecipitation, Molecular BiologyPierce Magnetic ChIP Kit
2013-08-29Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins.Lysis and ExtractionMem-PER Plus Membrane Protein Extraction Kit
2013-08-19Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents. Measure protein and peptide cysteine modifications using multiplex quantitative mass spectrometry.Mass Spectrometry, Post-translational ModificationiodoTMTsixplex Label Reagent Set, 1 x 0.2 mg
2013-08-02Performance characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins.Fusion Tag Methods, Affinity PurificationPierce Glutathione Superflow Agarose
2013-07-11Performance characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins.Fusion Tag Methods, Affinity PurificationHisPur Ni-NTA Superflow Agarose
2013-06-26Improved Western blot detection using CCD digital imaging. Comparison of cooled-CCD digital imaging versus X-ray film for sensitivity, dynamic range and signal linearity with enhanced chemiluminescence (ECL).Western Blotting, Electrophoresis and StainingmyECL Imager
2013-06-24Versatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes.Luciferase AssaysPierce Gaussia Luciferase Flash Assay Kit
2013-06-05Carrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity.Antibody ProductionImject mcKLH (in PBS)
2013-06-04A versatile mass spectrometry sample preparation procedure for complex protein samples. Prepare high-quality peptide digests with high recovery from cultured cells.Mass Spectrometry, Sample PreparationPierce Mass Spec Sample Prep Kit for Cultured Cells
2013-05-22Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates.Protein Expression1-Step Human Coupled IVT Kit - DNA
2013-05-02Performance characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin.Fusion Tag Methods, Affinity PurificationHisPur Cobalt Superflow Agarose
2013-04-30Activation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells.Luciferase AssaysPierce Gaussia Luciferase Flash Assay Kit
2013-04-18Separation characteristics of dialysis membranes. Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing.Dialysis and DesaltingSlide-A-Lyzer G2 Dialysis Cassettes, 10K MWCO, 3 mL
2013-03-08Monitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging.Luciferase Assays, IHC and Cell ImagingPierce Cypridina Luciferase Flash Assay Kit
2013-03-07Chromatography cartridges for desalting and affinity purification. Data using the cartridge format for automated liquid chromatography (LC) systems.Affinity Purification, Dialysis and DesaltingZeba Micro Spin Desalting Columns, 7K MWCO, 75 µL
2013-01-24Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader.Luciferase Assays, Protein ExpressionPierce Cypridina-Firefly Luciferase Dual Assay Kit
2012-12-11Quantitative cancer stem cell phosphoprotein profiling. Using tandem mass labeling and LC-MS/MS to profile perturbations of an autocrine loop.Mass Spectrometry, Post-translational ModificationPierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL
2012-11-12Phosphoprotein enrichment from cell and tissue samples. Isolate phosphorylated proteins with an easy-to-use spin formatPost-translational Modification, Affinity PurificationPierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL
2012-10-11Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry.Mass Spectrometry1-Step Heavy Protein IVT Kit
2012-07-18Eliminate endotoxins from protein and antibody samples. A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges.Sample PreparationPierce High Capacity Endotoxin Removal Resin
2012-07-09Removing endotoxins using a spin-column format. Fast and effective removal of endotoxins from protein samples.Sample PreparationPierce High Capacity Endotoxin Removal Resin
2012-06-13Luciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency.Luciferase AssaysPierce Gaussia Luciferase Flash Assay Kit
2012-05-18Fractionate subcellular proteins from tissue samples. Obtain proteins from five cellular components using a tissue-optimized kit.Lysis and ExtractionSubcellular Protein Fractionation Kit for Tissues
2012-05-10Method to isolate functional synaptosomes. Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity.Lysis and ExtractionSyn-PER Synaptic Protein Extraction Reagent
2012-04-18Complete protein protection from proteases and phosphatases. Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation.Lysis and Extraction, Sample PreparationPierce Protease Inhibitor Tablets, EDTA-free
2012-03-05Optimized protein extraction reagent for neuronal tissue and primary cells. Obtain high yields of functional neuronal protein from all neuronal cell compartments.Lysis and ExtractionN-PER Neuronal Protein Extraction Reagent
2012-02-28Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays.Luciferase Assays, Protein ExpressionPierce Cypridina-Firefly Luciferase Dual Assay Kit
2012-02-24Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale.Antibody Purification, Affinity PurificationPierce Protein A Plus Agarose
2011-10-07Improved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer.Immunoprecipitation, Protein ImmobilizationGlycoLink IP Kit
2011-06-01Western blotting using enhanced chemiluminescence. Choose the perfect substrate for your needs.Western BlottingPierce ECL Western Blotting Substrate
2011-06-01Serine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases.Protein Labeling, Protein AssaysActivX TAMRA-FP Serine Hydrolase Probe
2011-06-01Selective enrichment of sialylated glycopeptides using TiO2 beads. Affinity purification of glycoproteins for mass spectrometry based on titanium dioxide beads (TiO2).Mass SpectrometryPierce TiO2 Phosphopeptide Enrichment and Clean-up Kit
2011-06-01Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications.Immunoprecipitation, Affinity PurificationPierce Protein A/G Magnetic Beads
2011-06-01Optimize liquid chromatography with a peptide retention time calibration mixture. New mixture of 15 heavy peptides streamlines assay design for targeted peptide quantification.Chromatography, Mass SpectrometryPierce Peptide Retention Time Calibration Mixture
2011-06-01Calibration solutions for Thermo Scientific Mass Spectrometers. Ready-to-use standards enable quick instrument calibration.Mass SpectrometryPierce LTQ ESI Positive Ion Calibration Solution
2011-06-01New dimensions for low-volume sample dialysis. Self-contained devices give you the solution for high-protein recovery.Dialysis and Desalting, Sample PreparationSlide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL
2011-06-01Introducing a Western blot substrate that is chemiluminescent and chemifluorescent. Detect your target by film, CCD cameras or laser-based imagers.Western BlottingPierce ECL Plus Western Blotting Substrate
2011-06-01Develop mid- to high-throughput SRM and MRM assays. New application-specific peptide libraries for quantitative mass spectrometry workflows.Peptides, Mass SpectrometryPEPotec SRM Custom Peptide Libraries
2011-04-05Measure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates.Protein Assays, ImmunoprecipitationActive Rac1 Pull-Down and Detection Kit
2011-04-05GTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases.Protein Labeling, Affinity PurificationPierce GTPase Enrichment Kit with GTP Probe
2011-04-05Enhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody.IHC and Cell ImagingPierce Immunostain Enhancer
2011-04-05Detection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies.IHC and Cell Imaging, Affinity PurificationActive Rac1 Pull-Down and Detection Kit