2017-04-20 |
ProcartaPlex Human Immuno-Oncology Checkpoint Panel: detection of soluble isoforms of immuno-oncology checkpoint markers |
Multiplex Immunoassay |
ProcartaPlex Human Immuno-Oncology Checkpoint Panel |
2016-09-20 |
Characterization of antibody specificity using immunoprecipitation and mass spectrometry (IP-MS antibody validation). |
Antibody Production, Mass Spectrometry, Immunoprecipitation |
Antibodies |
2016-07-01 |
Optimizing protein A purification of monoclonal antibodies when using the ExpiCHO Expression System. |
Affinity Purification, Antibody Production |
ExpiCHO Expression System |
2015-12-15 |
Guide to gels and transfer methods for high molecular weight proteins, featuring the Pierce Power Blotter. |
Electrophoresis and Staining, Western Blotting |
Pierce Power Blotter |
2015-11-02 |
Pierce Power Stainer: a fast, reliable device for Coomassie protein staining and destaining. |
Electrophoresis and Staining, Western Blotting |
Pierce Power Stainer |
2014-05-22 |
Transfer EMSA gels using the Pierce G2 Fast Blotter. The Pierce G2 Fast Blotter provides rapid semi-dry transfer of DNA (TBE) gels to nylon membrane. |
Electrophoresis and Staining, Western Blotting |
Pierce G2 Fast Blotter – Power Stainer Upgrade |
2014-05-19 |
An improved method for highly efficient isolation of primary mouse embryonic fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts (MEF). |
Lysis and Extraction |
Pierce Mouse Embryonic Fibroblast Isolation Kit |
2014-05-02 |
Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system. A cell-based, quantitative co-immunoprecipitation method for protein interaction analysis involving a sensitive luciferase assay strategy. |
Immunoprecipitation, Luciferase Assays |
Pierce Agarose qIP Protein Interaction Kit, Tluc and HA Tags |
2014-04-02 |
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts. Easily isolate and culture highly viable, functional primary cardiomyocytes. |
Lysis and Extraction |
Pierce Primary Cardiomyocyte Isolation Kit |
2014-03-24 |
Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System. |
Protein Expression |
1-Step Human Coupled IVT Kit - DNA |
2014-03-24 |
Benchmarking experiments with Zeba Spin Desalting Columns. Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns. |
Dialysis and Desalting |
Zeba Micro Spin Desalting Columns, 7K MWCO, 75 µL |
2014-03-03 |
Calculate the number of immobilized proteins per bead of agarose affinity supports. A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands. |
Affinity Purification |
AminoLink Plus Coupling Resin |
2014-02-19 |
Improved, all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells. |
Lysis and Extraction |
B-PER Complete Bacterial Protein Extraction Reagent |
2014-01-28 |
An efficient method for the isolation of highly functional primary neurons. Easily and reproducibly isolate and culture primary neurons. |
Lysis and Extraction |
Pierce Primary Neuron Isolation Kit |
2014-01-16 |
Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria. |
Protein Expression |
1-Step Human High-Yield Mini IVT Kit |
2014-01-10 |
System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates. |
Protein Expression |
1-Step Human High-Yield Mini IVT Kit |
2013-11-12 |
Luciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers. |
Luciferase Assays |
Pierce Renilla Luciferase Flash Assay Kit |
2013-10-22 |
Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system. |
Protein Expression, Fusion Tag Methods |
pT7CFE1-NHis Vector for Mammalian Cell-Free Protein Expression |
2013-09-11 |
Analysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit. |
Immunoprecipitation, Molecular Biology |
Pierce Magnetic ChIP Kit |
2013-08-29 |
Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins. |
Lysis and Extraction |
Mem-PER Plus Membrane Protein Extraction Kit |
2013-08-19 |
Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents. Measure protein and peptide cysteine modifications using multiplex quantitative mass spectrometry. |
Mass Spectrometry, Post-translational Modification |
iodoTMTsixplex Label Reagent Set, 1 x 0.2 mg |
2013-08-02 |
Performance characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins. |
Fusion Tag Methods, Affinity Purification |
Pierce Glutathione Superflow Agarose |
2013-07-11 |
Performance characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins. |
Fusion Tag Methods, Affinity Purification |
HisPur Ni-NTA Superflow Agarose |
2013-06-26 |
Improved Western blot detection using CCD digital imaging. Comparison of cooled-CCD digital imaging versus X-ray film for sensitivity, dynamic range and signal linearity with enhanced chemiluminescence (ECL). |
Western Blotting, Electrophoresis and Staining |
myECL Imager |
2013-06-24 |
Versatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes. |
Luciferase Assays |
Pierce Gaussia Luciferase Flash Assay Kit |
2013-06-05 |
Carrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity. |
Antibody Production |
Imject mcKLH (in PBS) |
2013-06-04 |
A versatile mass spectrometry sample preparation procedure for complex protein samples. Prepare high-quality peptide digests with high recovery from cultured cells. |
Mass Spectrometry, Sample Preparation |
Pierce Mass Spec Sample Prep Kit for Cultured Cells |
2013-05-22 |
Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates. |
Protein Expression |
1-Step Human Coupled IVT Kit - DNA |
2013-05-02 |
Performance characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin. |
Fusion Tag Methods, Affinity Purification |
HisPur Cobalt Superflow Agarose |
2013-04-30 |
Activation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells. |
Luciferase Assays |
Pierce Gaussia Luciferase Flash Assay Kit |
2013-04-18 |
Separation characteristics of dialysis membranes. Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing. |
Dialysis and Desalting |
Slide-A-Lyzer G2 Dialysis Cassettes, 10K MWCO, 3 mL |
2013-03-08 |
Monitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging. |
Luciferase Assays, IHC and Cell Imaging |
Pierce Cypridina Luciferase Flash Assay Kit |
2013-03-07 |
Chromatography cartridges for desalting and affinity purification. Data using the cartridge format for automated liquid chromatography (LC) systems. |
Affinity Purification, Dialysis and Desalting |
Zeba Micro Spin Desalting Columns, 7K MWCO, 75 µL |
2013-01-24 |
Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader. |
Luciferase Assays, Protein Expression |
Pierce Cypridina-Firefly Luciferase Dual Assay Kit |
2012-12-11 |
Quantitative cancer stem cell phosphoprotein profiling. Using tandem mass labeling and LC-MS/MS to profile perturbations of an autocrine loop. |
Mass Spectrometry, Post-translational Modification |
Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL |
2012-11-12 |
Phosphoprotein enrichment from cell and tissue samples. Isolate phosphorylated proteins with an easy-to-use spin format |
Post-translational Modification, Affinity Purification |
Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL |
2012-10-11 |
Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry. |
Mass Spectrometry |
1-Step Heavy Protein IVT Kit |
2012-07-18 |
Eliminate endotoxins from protein and antibody samples. A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges. |
Sample Preparation |
Pierce High Capacity Endotoxin Removal Resin |
2012-07-09 |
Removing endotoxins using a spin-column format. Fast and effective removal of endotoxins from protein samples. |
Sample Preparation |
Pierce High Capacity Endotoxin Removal Resin |
2012-06-13 |
Luciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency. |
Luciferase Assays |
Pierce Gaussia Luciferase Flash Assay Kit |
2012-05-18 |
Fractionate subcellular proteins from tissue samples. Obtain proteins from five cellular components using a tissue-optimized kit. |
Lysis and Extraction |
Subcellular Protein Fractionation Kit for Tissues |
2012-05-10 |
Method to isolate functional synaptosomes. Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity. |
Lysis and Extraction |
Syn-PER Synaptic Protein Extraction Reagent |
2012-04-18 |
Complete protein protection from proteases and phosphatases. Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation. |
Lysis and Extraction, Sample Preparation |
Pierce Protease Inhibitor Tablets, EDTA-free |
2012-03-05 |
Optimized protein extraction reagent for neuronal tissue and primary cells. Obtain high yields of functional neuronal protein from all neuronal cell compartments. |
Lysis and Extraction |
N-PER Neuronal Protein Extraction Reagent |
2012-02-28 |
Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays. |
Luciferase Assays, Protein Expression |
Pierce Cypridina-Firefly Luciferase Dual Assay Kit |
2012-02-24 |
Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale. |
Antibody Purification, Affinity Purification |
Pierce Protein A Plus Agarose |
2011-10-07 |
Improved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer. |
Immunoprecipitation, Protein Immobilization |
GlycoLink IP Kit |
2011-06-01 |
Western blotting using enhanced chemiluminescence. Choose the perfect substrate for your needs. |
Western Blotting |
Pierce ECL Western Blotting Substrate |
2011-06-01 |
Serine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases. |
Protein Labeling, Protein Assays |
ActivX TAMRA-FP Serine Hydrolase Probe |
2011-06-01 |
Selective enrichment of sialylated glycopeptides using TiO2 beads. Affinity purification of glycoproteins for mass spectrometry based on titanium dioxide beads (TiO2). |
Mass Spectrometry |
Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit |
2011-06-01 |
Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications. |
Immunoprecipitation, Affinity Purification |
Pierce Protein A/G Magnetic Beads |
2011-06-01 |
Optimize liquid chromatography with a peptide retention time calibration mixture. New mixture of 15 heavy peptides streamlines assay design for targeted peptide quantification. |
Chromatography, Mass Spectrometry |
Pierce Peptide Retention Time Calibration Mixture |
2011-06-01 |
Calibration solutions for Thermo Scientific Mass Spectrometers. Ready-to-use standards enable quick instrument calibration. |
Mass Spectrometry |
Pierce LTQ ESI Positive Ion Calibration Solution |
2011-06-01 |
New dimensions for low-volume sample dialysis. Self-contained devices give you the solution for high-protein recovery. |
Dialysis and Desalting, Sample Preparation |
Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL |
2011-06-01 |
Introducing a Western blot substrate that is chemiluminescent and chemifluorescent. Detect your target by film, CCD cameras or laser-based imagers. |
Western Blotting |
Pierce ECL Plus Western Blotting Substrate |
2011-06-01 |
Develop mid- to high-throughput SRM and MRM assays. New application-specific peptide libraries for quantitative mass spectrometry workflows. |
Peptides, Mass Spectrometry |
PEPotec SRM Custom Peptide Libraries |
2011-04-05 |
Measure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates. |
Protein Assays, Immunoprecipitation |
Active Rac1 Pull-Down and Detection Kit |
2011-04-05 |
GTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases. |
Protein Labeling, Affinity Purification |
Pierce GTPase Enrichment Kit with GTP Probe |
2011-04-05 |
Enhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody. |
IHC and Cell Imaging |
Pierce Immunostain Enhancer |
2011-04-05 |
Detection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies. |
IHC and Cell Imaging, Affinity Purification |
Active Rac1 Pull-Down and Detection Kit |