Learn about methods and technologies for cell-based or cell-free protein production, including algal, bacterial, mammalian, insect, and yeast expression systems; and vector systems that are easy to use and deliver high protein yields for every downstream application.

  • Recombinant protein production
  • Host & vector systems
  • Cell-based vs. cell-free expression
  • Transformation
  • Cell culture
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Scientific posters
  • Support center resources
  • Protocols
  • Product selection guides

Protein expression features


This 118-page handbook provides comprehensive information about protein expression and will help you choose the right expression system and purification technologies for your specific application and needs.

Educational Hub

Gibco Protein Expression Basics is an online hub that brings together educational content and tools to enhance your research. Here you can access our protein expression selection guide, educational videos, and other technical resources. Start here if you are new to protein expression or need to refresh your knowledge.

Support center

One resource for all your protein expression support needs. Obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday problems related to protein expression. 

Protein expression learning resources

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Type Title
Handbook Protein Research Handbook (2016)
Handbook Protein Expression Handbook (2015)
Literature Gibco Expi protein expression systems brochure (Expi293 and ExpiCHO)
Literature ExpiSf expression system flyer
Literature Expi293 tools for structural biology and inducible expression
Methods article The Pierce Protein Methods Library
Methods article GST-tagged Proteins–Production and Purification
Methods article His-tagged Proteins–Production and Purification
Methods article Luciferase Reporters
Methods article An Overview of Post-Translational Modification
Methods article Phosphorylation
Methods article Glycosylation
Methods article An Overview of Protein Expression Systems
Methods article Cell-Free Protein Expression
Selection guide Protein Expression
Selection guide Luciferase Detection Instrumentation
Selection guide Luciferase Reporter Selection Guide
Selection guide Overview of Human in vitro Translation Systems
Selection guide Protein Expression Essentials
Selection guide Protein Expression Host Systems
Application note Scalable production and purification of adeno-associated virus (AAV) vector using the ExpiSf Expression System
Application note Adaptation of the ExpiSf Expression System for high-level protein production with the WAVE bioreactor system
Application note ExpiCHO Expression System—Protocols for 24 and 96 deep well blocks and mini bioreactor tubes
Application note ExpiCHO Expression System: A guide to optimizing protein A purification of monoclonal antibodies
Application note Adaptation of Expi293 to the WAVE Bioreactor System
Application note Expi293 MembranePro Expression System App Note
Application note The Expi293 Expression System—Optimizing protein yield
Application note Expi293 Expression System—Optimizing culture conditions to maximize protein yield and enable use of 96-well microtiter plates
Application note Using the Expi293 Expression System under animal origin–free (AOF) conditions
Application note Product Application Notes
Application note Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System.
Application note Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria.
Application note System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates.
Application note Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system.
Application note Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates.
Application note Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader.
Application note Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry.
Application note Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays.
Scientific poster Scalable protein and AAV production in the ExpiSf Expression System
Scientific poster ExpiCHO: Surpassing the Performance of Expi293 in a Transient CHO Expression System
Scientific poster Optimized expression of membrane proteins in the Expi293 and ExpiCHO expression systems: new tools for difficult to express proteins
Scientific poster Strategies for high-titer protein expression using the ExpiCHO and Expi293 transient expression systems
Scientific poster ExpiSf expression system: a chemically defined baculovirus-based system for enhanced protein and virus production in Sf9 cells 
Support center Protein Expression Support Center
Support center Adenoviral Gene Delivery for Mammalian Expression Support
Support center Algae Expression Support
Support center Bacterial Expression Support
Support center Basic Mammalian Expression Support
Support center Cell-Free Expression Support
Support center Insect Expression Support
Support center Lentiviral Gene Delivery for Mammalian Expression Support
Support center Mammalian Expression for High-Yield Protein Production Support
Support center Mammalian Expression for Membrane Protein Production Support
Support center Protein Expression Basics Support
Support center Reporter & Specialty Vectors for Mammalian Expression Support
Support center Yeast Expression Support
Protocol ExpiCHO essential protocol guide
Protocol Expi293 expression system user guide
Protocol ExpiSf smart start guide
Protocol Protein Expression Protocols
Protocol calculator Expi Systems Protocol Calculator
Webinar Enabling Next-Gen Targeted siRNA with ExpiCHO
Webinar Expression, purification and characterization in Expi293 and ExpiCHO
Webinar Factor V Expression in ExpiCHO Cells
Webinar Expi293: New Tools for structural Biology and difficult proteins
Webinar ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Webinar ExpressionWorld Webcasts