Learn about methods and technologies for cell-based or cell-free protein production, including algal, bacterial, mammalian, insect, and yeast expression systems; and vector systems that are easy to use and deliver high protein yields for every downstream application.

  • Recombinant protein production
  • Host & vector systems
  • Cell-based vs. cell-free expression
  • Transformation
  • Cell culture
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Scientific posters
  • Support center resources
  • Protocols
  • Product selection guides

Protein expression features


This 118-page handbook provides comprehensive information about protein expression and will help you choose the right expression system and purification technologies for your specific application and needs.

Educational Hub

Gibco Protein Expression Basics is an online hub that brings together educational content and tools to enhance your research. Here you can access our protein expression selection guide, educational videos, and other technical resources. Start here if you are new to protein expression or need to refresh your knowledge.

Support center

One resource for all your protein expression support needs. Obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday problems related to protein expression. 


The Protein Expression Publication Hub features a curated list of publications that offers an in-depth look into how scientists like you advance their research with Expi protein expression systems. Discover how you can achieve high-yield, consistent proteins for a diverse array of downstream applications today.

Protein expression learning resources

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HandbookProtein Research Handbook (2016)
HandbookProtein Expression Handbook (2015)
LiteratureGibco Expi protein expression systems brochure (Expi293 and ExpiCHO)
LiteratureExpiSf expression system flyer
LiteratureExpi293 tools for structural biology and inducible expression
Methods articleThe Pierce Protein Methods Library
Methods articleGST-tagged Proteins–Production and Purification
Methods articleHis-tagged Proteins–Production and Purification
Methods articleLuciferase Reporters
Methods articleAn Overview of Post-Translational Modification
Methods articlePhosphorylation
Methods articleGlycosylation
Methods articleAn Overview of Protein Expression Systems
Methods articleCell-Free Protein Expression
Selection guideProtein Expression
Selection guideLuciferase Detection Instrumentation
Selection guideLuciferase Reporter Selection Guide
Selection guideOverview of Human in vitro Translation Systems
Selection guideProtein Expression Essentials
Selection guideProtein Expression Host Systems
Application noteScalable production and purification of adeno-associated virus (AAV) vector using the ExpiSf Expression System
Application noteAdaptation of the ExpiSf Expression System for high-level protein production with the WAVE bioreactor system
Application noteExpiCHO Expression System—Protocols for 24 and 96 deep well blocks and mini bioreactor tubes
Application note ExpiCHO Expression System: A guide to optimizing protein A purification of monoclonal antibodies
Application noteAdaptation of Expi293 to the WAVE Bioreactor System
Application noteExpi293 MembranePro Expression System App Note
Application noteThe Expi293 Expression System—Optimizing protein yield
Application noteExpi293 Expression System—Optimizing culture conditions to maximize protein yield and enable use of 96-well microtiter plates
Application noteUsing the Expi293 Expression System under animal origin–free (AOF) conditions
Application noteProduct Application Notes
Application noteGlycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System.
Application noteExpression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria.
Application noteSystem for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates.
Application noteChoosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system.
Application noteProtocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates.
Application noteSimultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader.
Application noteCell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry.
Application noteHighly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays.
Scientific posterScalable protein and AAV production in the ExpiSf Expression System
Scientific posterExpiCHO: Surpassing the Performance of Expi293 in a Transient CHO Expression System
Scientific posterOptimized expression of membrane proteins in the Expi293 and ExpiCHO expression systems: new tools for difficult to express proteins
Scientific posterStrategies for high-titer protein expression using the ExpiCHO and Expi293 transient expression systems
Scientific posterExpiSf expression system: a chemically defined baculovirus-based system for enhanced protein and virus production in Sf9 cells 
Support centerProtein Expression Support Center
Support centerAdenoviral Gene Delivery for Mammalian Expression Support
Support centerAlgae Expression Support
Support centerBacterial Expression Support
Support centerBasic Mammalian Expression Support
Support centerCell-Free Expression Support
Support centerInsect Expression Support
Support centerLentiviral Gene Delivery for Mammalian Expression Support
Support centerMammalian Expression for High-Yield Protein Production Support
Support centerMammalian Expression for Membrane Protein Production Support
Support centerProtein Expression Basics Support
Support centerReporter & Specialty Vectors for Mammalian Expression Support
Support centerYeast Expression Support
ProtocolExpiCHO essential protocol guide
ProtocolExpi293 expression system user guide
ProtocolExpiSf smart start guide
ProtocolProtein Expression Protocols
Protocol calculatorExpi Systems Protocol Calculator
WebinarEnabling Next-Gen Targeted siRNA with ExpiCHO
WebinarExpression, purification and characterization in Expi293 and ExpiCHO
WebinarFactor V Expression in ExpiCHO Cells
WebinarExpi293: New Tools for structural Biology and difficult proteins
WebinarExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
WebinarExpressionWorld Webcasts

For Research Use Only. Not for use in diagnostic procedures.