Watch our learning series, which includes poster presentations and on-demand webinars (lectures), to receive firsthand knowledge of the latest technologies for protein gel electrophoresis and western blotting.
Webinar series: Simple steps to publication-quality western blots
Presented by: Jordan Thompson, Caleb Shearrow, and Kelli Feather-Henigan
Event date: Oct 12-14, 2021 | One session per day
Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reproducibility and quality of western blotting data has been increasingly scrutinized. Publications have developed more stringent review guidelines for western blot data, based on recommendations arising from a 2014 meeting organized by the National Institutes of Health (NIH), and the journals Science and Nature. Our team of western blotting experts have put together this 3-part webinar series that is designed to help you avoid some of the pitfalls associated with western blotting and enable you to achieve publication-quality western blot results. This webinar series will share tips and provide step-by-step guidance on how to improve the quality and reproducibility of data for both qualitative and quantitative western blotting, with attention on some of the newer guidelines.
Protein gel electrophoresis
Importance of sample preparation and choosing the right gel for SDS-PAGE
This webinar will discuss the importance of sample preparation in SDS-PAGE electrophoresis and how to choose the right gel chemistry for specific research application.
Choosing the right protein gel for your research application
This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications and how to introduce the right new gel chemistry in your research workflow.
Detecting low abundant proteins in a western blot: Overcoming challenges for improved signal-to-noise of low expressing and precious sample targets
Detection of low-abundance proteins and/or detection of proteins from limited volume samples can be a major challenge when performing western blotting. In this webinar, we will discuss specific methods from sample preparation through immunodetection that can help overcome challenges and improve signal-to-noise of low-abundance proteins for more successful western blot detection.
A novel method for quantitative western blotting that eliminates errors from the use of housekeeping proteins
In this webinar, we present data and analysis demonstrating the ease of use and improved accuracy and reproducibility of quantitative western blot data with No-Stain Protein Labeling Reagent compared to housekeeping proteins.
Light up your western blots – fluorescent western blotting tips, tricks & more
This webinar will highlight the fundamentals of fluorescent western blotting and discuss how to successfully design fluorescent western blotting experiments.
Poster presentation: Alternative blotting methods
This poster presentation will discuss the traditional wet blotting transfer technique as compared to dry and semi-dry transfer techniques.
Complete and innovative western workflow solutions: filling the gap between art and science (presented in Chinese)
This webinar will cover the electrophoresis, transfer, and detection steps of the western blotting workflow, highlight our popular products, and introduce tips and tricks for superior western analysis to make western blotting more efficient and effective.
Classical and modernized western blotting
This webinar will cover the electrophoresis, transfer, and detection steps of the western blotting workflow, in the framework of the classical approach and the modernized twists that make western blotting more efficient and effective.
Chemiluminescent western blot detection: Bright and bold detection no matter how scarce your target
In this webinar we will discuss the best methods for optimizing this technique as well as how to determine the appropriate reagents for your sensitive target detection.
For Research Use Only. Not for use in diagnostic procedures.