Watch and Learn

Western blotting is a multi-step process that can take hours or 1 to 2 days to complete, depending on the methods used. Below are videos that detail the common steps used to perform a western blot, from separating proteins in protein gel electrophoresis to developing the blot.

Separate proteins based on molecular weight.

Transfer the protein from gel to a solid-support membrane.

How to perform a western wet tank transfer using the Invitrogen Mini Blot Module

How to perform a western semi-dry transfer using the Invitrogen Power Blotter and the Select Transfer Stacks

How to perform a western blot semi-dry transfer using the Invitrogen Power Blotter with precut membranes and filter papers

How to perform a western blot dry transfer using the Invitrogen iBlot 2 Dry Blotting System

Block unreacted sites on the membrane to reduce the amount of nonspecific binding. Probe blot with primary and secondary antibodies.

How to automate your western blot processing using the Invitrogen iBind Western systems

Detect proteins based on the detection method that you choose.

How to detect your target proteins using chemiluminescent detection reagents

Capture and analyze your image.

How to capture images with Invitrogen iBright Imaging Systems

How to analyze images with Invitrogen iBright Imaging Systems

How to export images with Invitrogen iBright Imaging Systems

Stripping and reprobe the blot for another protein of interest.

How to strip and reprobe your western blot

Separate proteins based on molecular weight.

Transfer the protein from gel to a solid-support membrane.

How to perform a western wet tank transfer using the Invitrogen Mini Blot Module

How to perform a western semi-dry transfer using the Invitrogen Power Blotter and the Select Transfer Stacks

How to perform a western blot semi-dry transfer using the Invitrogen Power Blotter with precut membranes and filter papers

How to perform a western blot dry transfer using the Invitrogen iBlot 2 Dry Blotting System

Block unreacted sites on the membrane to reduce the amount of nonspecific binding. Probe blot with primary and secondary antibodies.

How to automate your western blot processing using the Invitrogen iBind Western systems

Detect proteins based on the detection method that you choose.

How to detect your target proteins using chemiluminescent detection reagents

Capture and analyze your image.

How to capture images with Invitrogen iBright Imaging Systems

How to analyze images with Invitrogen iBright Imaging Systems

How to export images with Invitrogen iBright Imaging Systems

Stripping and reprobe the blot for another protein of interest.

How to strip and reprobe your western blot

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