Learn about methods and technologies for protein labeling, modification, and crosslinking. Understand the chemical functional groups and reaction chemistries required to crosslink and conjugate proteins to one another; to label proteins with biotin, PEG, or fluorescent dyes; or to reduce, cleave, and fragment proteins for use in assays.
Protein modification and crosslinking features
Biological research often requires the use of molecular labels that are covalently attached to a protein of interest to facilitate detection or purification of the labeled protein and/or its binding partners. Labeling strategies result in the covalent attachment of different molecules, including biotin, reporter enzymes, and fluorophores.
Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Modification involves attaching or cleaving chemical groups to alter the solubility or other properties of the original molecule.
Protein modification and crosslinking learning resources
Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.
For Research Use Only. Not for use in diagnostic procedures.