Learn about methods and technologies for western blot detection and imaging. Understand the reagents and instruments used for the efficient membrane transfer and detection of proteins, including wash and blocking buffers, secondary antibody conjugates, and chemiluminescent, colorimetric or fluorescent detection substrates.
- Tank vs. semi-dry transfer
- Blotting membranes
- Nitrocellulose vs. PVDF
- Blocking buffers
- Washing buffers
- Detection probes
- Enzymes & substrates
- Chemiluminescent vs. fluorescent detection
- Technical handbooks
- Protein methods articles
- Product application notes
- Support center resources
- Product selection guides
Western blotting features
From separation to transfer and detection, this interactive guide helps you learn about each step so that you can select the right products for your western blotting application. Learning tools, including videos and handbooks can be accessed from this informative guide.
This 84-page handbook provides a deep dive about the last step in the western blot workflow—protein detection. With a variety of detection techniques to choose from (chemiluminescence, fluorescence or chromogenic), you can select a technology to match your experimental requirements and the instruments you have available.
Videos that demonstrate each step in the western blot workflow, including SDS-PAGE, transfer, blocking, probing with antibodies, chemiluminescent detection, and stripping the blot.
Western blotting learning resources
Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.
For Research Use Only. Not for use in diagnostic procedures.