Mammalian transient expression systems enable flexible and rapid production of proteins. They're ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more native folding and post-translational modifications—such as glycosylation—than expression systems based on hosts such as E. coli, yeast, or insect cells.
Choose the mammalian transient system that's right for you
|The most widely used transient expression system||Gram per liter protein yield 293-based system||Up to 3 g/L protein yield CHO-based system Most-cost effective|
|FreeStyle™ 293 System||Expi293™ System||ExpiCHO™ System|
|Media||FreeStyle™ 293 Medium||Expi293™ Medium||ExpiCHO™ Medium|
|Transfection reagent||293Fectin™||ExpiFectamine™ 293 Transfection Kit||ExpiFectamine™ CHO Transfection Kit|
|Positive control vector||pCMV•Sport-βgal||Antibody-expressing vector||Antibody-expressing vector|
|Maximum working culture Density||2 x 106/mL||6 x 106/mL||10 x 106/mL|
|Cell density for transfection||1 x 106/mL||2.5 x 106/mL||6 x 106/mL|
|Protein yield*||20–200 mg/L||Up to 1 g/L||Up to 3 g/L|
*Typical yield of human IgGs
For Research Use Only. Not for use in diagnostic procedures.