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Mammalian transient expression systems enable flexible and rapid production of proteins. They're ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more native folding and post-translational modifications—such as glycosylation—than expression systems based on hosts such as E. coli, yeast, or insect cells.

Choose the mammalian transient system that's right for you

  The most widely used transient expression system Gram per liter protein yield 293-based system Up to 3 g/L protein yield CHO-based system Most-cost effective
  FreeStyle™ 293 System Expi293™ System ExpiCHO™ System
Cells FreeStyle™ 293-F Expi293-F™ ExpiCHO-S™
Media FreeStyle™ 293 Medium Expi293™ Medium ExpiCHO™ Medium
Transfection reagent 293Fectin™ ExpiFectamine™ 293 Transfection Kit ExpiFectamine™ CHO Transfection Kit
Positive control vector pCMV•Sport-βgal Antibody-expressing vector Antibody-expressing vector
Maximum working culture Density 2 x 106/mL 6 x 106/mL 10 x 106/mL
Cell density for transfection 1 x 106/mL 2.5 x 106/mL 6 x 106/mL
Protein yield* 20–200 mg/L Up to 1 g/L Up to 3 g/L
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*Typical yield of human IgGs