Mammalian transient expression systems enable flexible and rapid production of proteins. They're ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more native folding and post-translational modifications—such as glycosylation—than expression systems based on hosts such as E. coli, yeast, or insect cells.
Choose the mammalian transient system that's right for you
Traditional transient 293 expression system
Highest-yield and most flexible 293-based system
Highest-yield and most cost-effective CHO-based system
|Protein yield*||20–200 mg/L||Up to 1 g/L||Up to 3 g/L|
|Media||FreeStyle 293 Medium||Expi293 Medium||ExpiCHO Medium|
|Transfection reagent||293Fectin||ExpiFectamine 293 Transfection Kit||ExpiFectamine CHO Transfection Kit|
|Positive control vector||pCMV•Sport-βgal||Antibody-expressing vector||Antibody-expressing vector|
|Maximum working culture Density||2 x 106/mL||6 x 106/mL||10 x 106/mL|
|Cell density for transfection||1 x 106/mL||2.5 x 106/mL||6 x 106/mL|
|*Typical yield of human IgGs|
For Research Use Only. Not for use in diagnostic procedures.